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Bioprocess and Biosystems Engineering

, Volume 42, Issue 1, pp 93–105 | Cite as

In situ biological CO2 fixation and wastewater nutrient removal with Neochloris oleoabundans in batch photobioreactor

  • S. A. Razzak
Research Paper
  • 39 Downloads

Abstract

Microalgae cultivation in wastewater media in phototrophic condition is a promising approach for integrated CO2 biofixation and wastewater treatment. For this, Neochloris oleoabundans was used to investigate the tertiary treatment of wastewater along with CO2 biofixation. In this investigation, biomass productivity, CO2 biofixation rate and percentage of total nitrogen (TN) and total phosphorus (TP) removal from synthetic wastewater are considered under three different operating conditions: temperature, CO2 feed concentration and nitrogen to phosphorus (NP) ratio in the media. Cultivation of N. oleoabundans was found to be highly temperature sensitive. With the increase of cultivation temperature from 25 to 45 °C, declining trends of biomass concentration, productivity and percentage of TN and TP removal were observed. Cultivation temperature of 25 °C was found to be most favorable in terms of biomass productivity, CO2 biofixation rate, percentage of TN and TP removal of 92 (mg L−1 day−1), 145 (mg L−1 day−1), 100% and 32%, respectively. Arrhenius-type kinetic model was used and the model showed good agreement with the experimental findings. Activation energy for the active stage and decay stage was found to be \({E_{\text{a}}}\) = 88.8 kJ mol−1 and \({E_{\text{d}}}\) = 8.4 kJ mol−1, respectively. With the increase of CO2 feed concentration, biomass productivity increased and the maximum biomass concentration and productivity was achieved at 6%. After that with the increase in CO2, a declining trend was observed. With the increase of NP ratio from 1:1 to 2:1, both the biomass productivity and CO2 biofixation were increased, but later were subsequently decreased with increase of NP ratio from 4:1 to 8:1. It is interesting that TP removal was increased with NP ratio and 100 percent of TP removal was achieved at 4:1 and 8:1 conditions.

Keywords

Microalgae Biomass CO2 biofixation Wastewater treatment Nutrient removal 

List of symbols

OD

Optical density

BBM

Bold’s basal medium

\({\mu _{\text{g}}}\)

Specific growth rate

\({\mu _{\text{m}}}\)

Maximum specific growth rate

\({P_{\text{B}}}\)

Biomass productivity (mg L−1 day−1)

\({X_1}\) and \({X_2}\)

Biomass weight (mg) at the time \({t_1}~\) and \({t_2}\)

\({X_{\text{t}}}\) and \({X_0}\)

Biomass weight (mg) at the initial time, \({t_0}\) and at the end of the cultivation period \({t_{\text{t}}}\)

\({R_{{\text{C}}{{\text{O}}_{\text{2}}}}}\)

CO2 biofixation rate (mg L−1 day−1)

\({C_{{\text{carbon}}}}\)

Carbon content

\({M_{C{O_2}}}\)

Molecular weight of CO2

\({M_{\text{c}}}\)

Molecular weight of carbon

T

Temperature (°C)

\({T_0}\)

Reference temperature (°C)

\({A_0},~{B_0}\)

Fittings parameters

\({S_0}\)

Substrate concentrations at the initial time, \({t_0}\)

\({S_{\text{t}}}\)

Substrate concentrations at the end of the cultivation period \({t_{\text{t}}}\)

\({E_{\text{a}}},~{E_{\text{d}}}\)

Activation energy at the active and decay phase

Notes

Acknowledgements

The author would like to gratefully acknowledge the support provided by King Abdulaziz City for Science and Technology (KACST) through the Science & Technology Unit at King Fahd University of Petroleum & Minerals (KFUPM) for funding this work through project No. NSTIP # 13-WAT96-04 as part of the National Science, Technology and Innovation Plan.

Supplementary material

449_2018_2017_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 13 KB)
449_2018_2017_MOESM2_ESM.docx (17 kb)
Supplementary material 2 (DOCX 16 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemical EngineeringKing Fahd University of Petroleum and MineralsDhahranSaudi Arabia

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