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Bioprocess and Biosystems Engineering

, Volume 42, Issue 1, pp 71–81 | Cite as

Fermentation performance and metabolomic analysis of an engineered high-yield PUFA-producing strain of Schizochytrium sp.

  • Lingjun Geng
  • Shenglan Chen
  • Xiaoman Sun
  • Xuechao Hu
  • Xiaojun Ji
  • He Huang
  • Lujing RenEmail author
Research Paper
  • 137 Downloads

Abstract

The ω-3/long-chain polyunsaturated fatty acids (LC-PUFAs) play an important role in human health, but they cannot be synthesized in sufficient amounts by the human body. In a previous study, we obtained an engineered Schizochytrium sp. strain (HX-RS) by exchanging the acyltransferase (AT) gene, and it was able to co-produce docosahexaenoic acid and eicosapentaenoic acid. To investigate the mechanism underlying the increase of PUFA content in HX-RS, the discrepancies of fermentation performance, key enzyme activities and intracellular metabolites between HX-RS and its wild-type parent strain (WTS) were analyzed via fed-batch fermentation in 5-L bioreactors. The results showed that the cell dry weight (CDW) of HX-RS was higher than that of the WTS. Metabolomics combined with multivariate analysis showed that 4-aminobutyric acid, proline and glutamine are potential biomarkers associated with cell growth and lipid accumulation of HX-RS. Additionally, the shift of metabolic flux including a decrease of glyceraldehyde-3-phosphate content, high flux from pyruvate to acetyl-CoA, and a highly active glycolysis pathway were also found to be closely related to the high PUFA yield of the engineered strain. These findings provide new insights into the effects of exogenous AT gene expression on cell proliferation and fatty acid metabolism.

Keywords

Polyunsaturated fatty acids Schizochytrium sp. Fed batch Metabolomics 

Notes

Acknowledgements

This work was financially supported by the Outstanding Youth Foundation of Jiangsu Natural Science Foundation (BK20160092), the National Natural Science Foundation of China (No. 21878151), the Program for Innovative Research Teams in Universities of Jiangsu Province (2015) and Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTE1829).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Lingjun Geng
    • 2
  • Shenglan Chen
    • 2
  • Xiaoman Sun
    • 2
  • Xuechao Hu
    • 1
    • 2
  • Xiaojun Ji
    • 1
    • 2
  • He Huang
    • 1
    • 3
    • 4
  • Lujing Ren
    • 1
    • 2
    Email author
  1. 1.Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)NanjingChina
  2. 2.College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjingPeople’s Republic of China
  3. 3.School of Pharmaceutical SciencesNanjing Tech UniversityNanjingPeople’s Republic of China
  4. 4.State Key Laboratory of Materials-Oriented Chemical EngineeringNanjing Tech UniversityNanjingPeople’s Republic of China

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