Enhanced biocatalytic activity of immobilized steapsin lipase in supercritical carbon dioxide for production of biodiesel using waste cooking oil
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The present work reports covalent immobilization of steapsin lipase (SL) on Immobead-350 support matrix (IMB) to make a robust biocatalytic system to work under supercritical carbon dioxide condition (Sc-CO2). The developed biocatalyst (IMB:SL) was characterized in details and utilized to convert waste cooking sunflower oil (WCSO) into value-added energy chemical (biodiesel) in Sc-CO2. All reaction process parameters were optimized in detail which offered 86.33% yield of biodiesel from WCSO. The developed Sc-CO2 protocol is compared with the solvent-free conventional synthesis, which indicates almost twofold higher yield of biodiesel in Sc-CO2 media as compared to solvent-free condition. To extend the scope, we have tested fresh and waste cooking oils (WCO) from various sources, offering 81–94% yield of biodiesel. The biocatalyst activity was investigated in various parameters of supercritical condition to know the biocatalyst stability in Sc-CO2. Besides this, IMB:SL biocatalyst was effectively reused up to five recycle.
KeywordsBiocatalysis Biodiesel synthesis Waste feedstock Waste cooking oil Steapsin in Sc-CO2
The author VCB and KCB are greatly thankful to ChiralVision, The Netherlands, who offered Immobeads 350 (IMB) as a generous gift sample for the research study. In addition, the author VCB is greatly thankful to the Rajiv Gandhi Science and Technology Commission (RGS & TC) research funding scheme, conducted by North Maharashtra University (NMU), Jalgaon, for their financial support (Ref. No: NMU/11/RGS&TC/438/215; Project Code: 51-ENV_LSR).
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Conflict of interest
The authors declare that they have no conflict of interest.
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