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Bioprocess and Biosystems Engineering

, Volume 42, Issue 1, pp 47–61 | Cite as

Enhanced biocatalytic activity of immobilized steapsin lipase in supercritical carbon dioxide for production of biodiesel using waste cooking oil

  • Vivek C. Badgujar
  • Kirtikumar C. Badgujar
  • Pravin M. Yeole
  • Bhalchandra M. BhanageEmail author
Research Paper
  • 41 Downloads

Abstract

The present work reports covalent immobilization of steapsin lipase (SL) on Immobead-350 support matrix (IMB) to make a robust biocatalytic system to work under supercritical carbon dioxide condition (Sc-CO2). The developed biocatalyst (IMB:SL) was characterized in details and utilized to convert waste cooking sunflower oil (WCSO) into value-added energy chemical (biodiesel) in Sc-CO2. All reaction process parameters were optimized in detail which offered 86.33% yield of biodiesel from WCSO. The developed Sc-CO2 protocol is compared with the solvent-free conventional synthesis, which indicates almost twofold higher yield of biodiesel in Sc-CO2 media as compared to solvent-free condition. To extend the scope, we have tested fresh and waste cooking oils (WCO) from various sources, offering 81–94% yield of biodiesel. The biocatalyst activity was investigated in various parameters of supercritical condition to know the biocatalyst stability in Sc-CO2. Besides this, IMB:SL biocatalyst was effectively reused up to five recycle.

Keywords

Biocatalysis Biodiesel synthesis Waste feedstock Waste cooking oil Steapsin in Sc-CO2 

Notes

Acknowledgements

The author VCB and KCB are greatly thankful to ChiralVision, The Netherlands, who offered Immobeads 350 (IMB) as a generous gift sample for the research study. In addition, the author VCB is greatly thankful to the Rajiv Gandhi Science and Technology Commission (RGS & TC) research funding scheme, conducted by North Maharashtra University (NMU), Jalgaon, for their financial support (Ref. No: NMU/11/RGS&TC/438/215; Project Code: 51-ENV_LSR).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Gharat N, Rathod VK (2013) Enzyme catalyzed transesterification of waste cooking oil with dimethyl carbonate. J Mol Catal B Enzym 88:36–40CrossRefGoogle Scholar
  2. 2.
    Badgujar KC, Bhanage BM (2015) Factors governing dissolution process of lignocellulosic biomass in ionic liquid: current status, overview and challenges. Biores Technol 178:2–18CrossRefGoogle Scholar
  3. 3.
    Badgujar KC, Bhanage BM (2016) The green metric evaluation and synthesis of diesel-blend compounds from biomass derived levulinic acid in supercritical carbon dioxide. Biomass Bioenergy 84:12–21CrossRefGoogle Scholar
  4. 4.
    Badgujar KC, Bhanage BM (2015) Thermo-chemical energy assessment for production of energy-rich fuel additive compounds by using levulinic acid and immobilized lipase. Fuel Process Technol 138:139–146CrossRefGoogle Scholar
  5. 5.
    Barnwal BK, Sharma MP (2005) Prospects of biodiesel production from vegetable oils in India. Renew Sustain Energy Rev 9:363–378CrossRefGoogle Scholar
  6. 6.
    US-EPA-RFS (2005) United State-environmental protection agency—renewable fuel standard. http://www.epa.gov/otaq/fuels/renewablefuels/. Accessed 22 April 2018
  7. 7.
    Corma A, Iborra S, Velty A (2007) Chemical routes for the transformation of biomass into chemicals. Chem Rev 107:2411–2502CrossRefGoogle Scholar
  8. 8.
    Badgujar KC, Bhanage BM (2018) Waste feedstocks for biofefineries: an approach to develop a sustainable society waste biorefinery: potential and perspectives, 1st edn. Elsevier, Amsterdam. ISBN: 9780444639936Google Scholar
  9. 9.
    Naik SN, Goud VV, Rout PK, Dalai AK (2010) Production of first and second generation biofuels: a comprehensive review. Renew Sustain Energy Rev 14:578–597CrossRefGoogle Scholar
  10. 10.
  11. 11.
    Lam SS, Liew RK, Jusoh A, Chong CT, Chase HA (2016) Progress in waste oil to sustainable energy, with emphasis on pyrolysis techniques. Renew Sustain Energy Rev 53:741–753CrossRefGoogle Scholar
  12. 12.
    Araújo CDM, Andrade CC, Silva ES, Dupas FA (2013) Biodiesel production from used cooking oil: a review. Renew Sustain Energy Rev 27:445–452CrossRefGoogle Scholar
  13. 13.
    Charpe TW, Rathod VK (2011) Biodiesel production using waste frying oil. Waste Manag 31:85–90CrossRefGoogle Scholar
  14. 14.
    Paola M, Calabrò ERV, Curcio S, Iorio G (2009) Factor analysis of transesterification reaction of waste oil for biodiesel production. Biores Technol 100:5126–5131CrossRefGoogle Scholar
  15. 15.
    Pizarro AVL, Park EY (2003) Lipase-catalyzed production of biodiesel fuel from vegetable oils contained in waste activated bleaching earth. Process Biochem 38:1077–1082CrossRefGoogle Scholar
  16. 16.
    Kailie N, Feng X, Fang W, Tianwie T (2003) Lipase catalyzed methanolysis to produce biodiesel: optimization of the biodiesel production. J Mol Catal B Enzym 43:142–147Google Scholar
  17. 17.
    Omar WNNW, Amin NAS (2011) Biodiesel production from waste cooking oil over alkaline modified zirconia catalyst. Fuel Process Technol 92:2397–2405CrossRefGoogle Scholar
  18. 18.
    Hameed BH, Goh CS, Chin LH (2009) Fuel Process optimization for methyl ester production from waste cooking oil using activated carbon supported potassium fluoride. Fuel Process Technol 90:1532–1537CrossRefGoogle Scholar
  19. 19.
    Lin Y, Yang P, Chen S, Lin J (2013) Improving biodiesel yields from waste cooking oil using ionic liquids as catalysts with a microwave heating system. Fuel Process Technol 115:57–62CrossRefGoogle Scholar
  20. 20.
    DaRós PC, Freitas L, Perez VH, de Castro HF (2013) Enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation. Bioprocess Biosyst Eng 36:443–451CrossRefGoogle Scholar
  21. 21.
    Dhake KP, Bhatte KD, Wagh YS, Bhanage BM (2012) Immobilization of steapsin lipase on macroporous immobead-350 for biodiesel production in solvent free system. Biotechnol Bioproc Eng 17:959–965CrossRefGoogle Scholar
  22. 22.
    Trentin CM, Popiolki AS, Batistella L et al (2015) Enzyme-catalyzed production of biodiesel by ultrasound-assisted ethanolysis of soybean oil in solvent-free system. Bioprocess Biosyst Eng 38:437–448CrossRefGoogle Scholar
  23. 23.
    Calabrò V, Ricca E, De Paola MG, Curcio S, Iorio G (2010) Kinetics of enzymatic trans-esterification of glycerides for biodiesel production. Bioprocess Biosyst Eng 33:701–710CrossRefGoogle Scholar
  24. 24.
    Badgujar KC, Bhanage BM (2015) Carbohydrate base co-polymers as an efficient immobilization matrix to enhance lipase activity for potential biocatalytic applications. Carbohydr Polym 134:709–717CrossRefGoogle Scholar
  25. 25.
    Badgujar KC, Bhanage BM (2014) Application of lipase immobilized on the biocompatible ternary blend polymer matrix for synthesis of citronellyl acetate in non-aqueous media: kinetic modelling study. Enzym Microbial Technol 57:16–25CrossRefGoogle Scholar
  26. 26.
    Lima LN, Aragon CC, Mateo C, Palomo JM, Fernandez-Lorente G (2013) Immobilization and stabilization of a bimolecular aggregate of the lipase from Pseudomonas fluorescens by multipoint covalent attachment. Process Biochem 48:118–123CrossRefGoogle Scholar
  27. 27.
    Boros Z, Weiser D, Márkus M, Abaháziová E, Poppe L (2013) Hydrophobic adsorption and covalent immobilization of Candida antarctica lipase B on mixed-function-grafted silica gel supports for continuous-flow biotransformations. Process Biochem 48:1039–1047CrossRefGoogle Scholar
  28. 28.
    Wang W, Zhou W, Li J, Hao D, Su Z, Ma G (2015) Comparison of covalent and physical immobilization of lipase in gigaporous polymeric microspheres. Bioprocess Biosyst Eng 38:2107–2115CrossRefGoogle Scholar
  29. 29.
    Lima LCD, Peres DGC, Mendes AA (2018) Kinetic and thermodynamic studies on the enzymatic synthesis of wax ester catalyzed by lipase immobilized on glutaraldehyde-activated rice husk particles. Just accepted manuscript. Bioprocess Biosyst Eng.  https://doi.org/10.1007/s00449-018-1929-9 Google Scholar
  30. 30.
    Halim SFA, Kamaruddin AH (2008) Catalytic studies of lipase on FAME production from waste cooking palm oil in a tert-butanol system. Process Biochem 43:1436–1439CrossRefGoogle Scholar
  31. 31.
    Bernal JM, Lozano P (2012) Supercritical synthesis of biodiesel. Molecule 17:8696–8719CrossRefGoogle Scholar
  32. 32.
    Badgujar KC, Bhanage BM (2015) Immobilization of lipase on biocompatible co-polymer of polyvinyl alcohol and chitosan for synthesis of laurate compounds in supercritical carbon dioxide using response surface methodology. Process Biochem 50:1224–1236CrossRefGoogle Scholar
  33. 33.
    Pencreacha G, Barattia JC (2001) Comparison of hydrolytic activity in water and heptane for thirty-two commercial lipase preparations. Enzym Microb Technol 28:473–479CrossRefGoogle Scholar
  34. 34.
    Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  35. 35.
    Badgujar KC, Bhanage BM (2014) Enhanced biocatalytic activity of lipase immobilized on biodegradable copolymer of chitosan and polyvinyl alcohol support for synthesis of propionate ester: kinetic approach. Ind Eng Chem Res 53:18806–18815CrossRefGoogle Scholar
  36. 36.
    Mueanmas C, Prasertsit K, Tongurai C (2010) Feasibility study of reactive distillation column for transesterification of palm oils. Inter J Chem Eng Appl 1:77–83Google Scholar
  37. 37.
    Bayramoglu G, Yilmaz M, Yakup AM (2010) Preparation and characterization of epoxy-functionalized magnetic chitosan beads: laccase immobilized for degradation of reactive dyes. Bioprocess Biosyst Eng 33:439–448CrossRefGoogle Scholar
  38. 38.
    Badgujar KC, Sasaki T, Bhanage BM (2015) Synthesis of lipase nano-bio-conjugates as an efficient biocatalyst: characterization and activity-stability studies with potential biocatalytic applications. RSC Adv 5:55238–55251CrossRefGoogle Scholar
  39. 39.
    Badgujar KC, Bhanage BM (2017) Investigation of deactivation thermodynamics of lipase immobilized on polymeric carrier. Bioprocess Biosyst Eng 40:74–757Google Scholar
  40. 40.
    Cieh NJ, Sulaiman S, Mokhtar MN, Naim MN (2017) Bleached kenaf microfiber as a support matrix for cyclodextrin glucanotransferase immobilization via covalent binding by different coupling agents. Process Biochem 56:81–89CrossRefGoogle Scholar
  41. 41.
    Bai Y, Li Y, Yang Y, Yi L (2006) Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres. Process Biochem 41:770–777CrossRefGoogle Scholar
  42. 42.
    Mateo C, Abian O, Lafuente R, Guisan JM (2000) Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment. Enzym Microbial Technol 26:509–515CrossRefGoogle Scholar
  43. 43.
    Mateo C, Palomo JM, Lorente G, Guisan JM, Lafuente R (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzym Microbial Technol 40:1451–1463CrossRefGoogle Scholar
  44. 44.
    Badgujar KC, Bhanage BM (2016) Lipase immobilization on hyroxypropyl methyl cellulose support and its applications for chemo-selective synthesis of β-amino ester compounds. Process Biochem 51:1420–1433CrossRefGoogle Scholar
  45. 45.
    Bayramogu G, Hazer GB, Altıntas B, Arıca MY (2011) Covalent immobilization of lipase onto amine functionalized polypropylene membrane and its application in green apple flavor (ethyl valerate) synthesis. Process Biochem 46:372–378CrossRefGoogle Scholar
  46. 46.
    Ramani K, Karthikeyan S, Boopathy R, Kennedy LG, Sekaran G (2012) Surface functionalized mesoporous activated carbon for the immobilization of acidic lipase and their application to hydrolysis of waste cooked oil: isotherm and kinetic studies. Process Biochem 47:435–445CrossRefGoogle Scholar
  47. 47.
    Raita M, Arnthog J, Champred V, Laosiripojan N, Modification of magnetic nanoparticle lipase designs for biodiesel production. Fuel Process Technol.134:189–197Google Scholar
  48. 48.
    Nelson DL (2008) Lehninger’s principles of biochemistry, 5th edn. ISBN 10:071677108XGoogle Scholar
  49. 49.
    Osuna Y, Sandoval J, Saade H et al (2015) Immobilization of Aspergillus niger lipase on chitosan-coated magnetic nanoparticles using two covalent-binding methods. Bioprocess Biosyst Eng 38:1437–1445CrossRefGoogle Scholar
  50. 50.
    Wen D, Jiang H, Zhang K (2009) Supercritical fluids technology for clean biofuel production. Prog Natl Sci 19:273–284CrossRefGoogle Scholar
  51. 51.
    Pollardo AA et al (2017) Effect of supercritical carbon dioxide on the enzymatic production of biodiesel from waste animal fat using immobilized candida antarctica lipase B variant. BMC Biotechnol 17:70–78CrossRefGoogle Scholar
  52. 52.
    Lee M. Lee J, Lee D, Cho J, Kim S, Park C (2011) Improvement of enzymatic biodiesel production by controlled substrate feeding using silica gel in solvent free system. Enzyme Microbial Technol 49:402–406CrossRefGoogle Scholar
  53. 53.
    Robles-Medina A, Gonzalez-Moreno PA, Esteban-Cerdan L, Molina-Grima E (2009) Biocatalysis: towards ever greener biodiesel production. Biotechnol Adv 27:398–408CrossRefGoogle Scholar
  54. 54.
    Habulin M, Šabeder S, Paljeva M, Primozic M, Knez Z (2007) Lipase-catalyzed esterification of citronellol with lauric acid in supercritical carbon dioxide/co-solvent media. J Supercrit Fluid 43:199–203CrossRefGoogle Scholar
  55. 55.
    Royon D, Daz M, Ellenrieder G, Locatelli S (2007) Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent. Biores Technol 98:648–653CrossRefGoogle Scholar
  56. 56.
    Ghaziaskar HS, Rezayat M. Butanol structure-solubility relationship in supercritical carbon dioxide report. http://www.isasf.net/fileadmin/files/Docs/Versailles/Papers/PTs32.pdf. Accessed on 10 April 2017.
  57. 57.
    Oliveira JVOD (2000) Kinetics of the enzymatic alcoholysis of palm kernel oil in supercritical CO2. Ind Eng Chem Res 39:4450–4454CrossRefGoogle Scholar
  58. 58.
    Ciftci ON, Temelli F (2011) Continuous production of fatty acid methyl esters from corn oil supercritical carbon dioxide bioreactor. J Supercrit Fluid 58:79–87CrossRefGoogle Scholar
  59. 59.
    Lee M, Cho J, Kim S, Lee D, Han J, Cho JK, Park C (2012) Improved high-pressure enzymatic biodiesel batch synthesis in near-critical carbon dioxide. Bioprocess Biosyst Eng 35:105–113CrossRefGoogle Scholar
  60. 60.
    Lee JH, Kwon CH, Kang JW, Park C, Tae B, Kim SW (2009) Biodiesel production from various oils under supercritical fluid conditions by Candida antarctica lipase B using a stepwise reaction method. Appl Biochem Biotechnol 156:454–464CrossRefGoogle Scholar
  61. 61.
    Lozano P, García-Verdugo E, Piamtongkam R, Karbass N, Diego TD, Burguete MI, Luis SV, Iborr JL. Bioreactors based on monolith-supported ionic liquid phase for enzyme catalysis in supercritical carbon dioxide. Adv Synth Catal 349:1077–1084Google Scholar
  62. 62.
    Badgujar KC, Bhanage BM (2014) The solvent stability study with thermodynamic analysis and superior biocatalytic activity of burkholderia cepacia lipase immobilized on biocompatible hybrid matrix of polyvinyl alcohol and hypromellose. J Phys Chem B 118:14808–14819Google Scholar
  63. 63.
    Badgujar KC, Pai PA, Bhanage BM (2016) Enhanced biocatalytic activity of immobilized Pseudomonas cepacia lipase under sonicated condition. Bioprocess Biosyst Eng 39:211–221CrossRefGoogle Scholar
  64. 64.
    Tupufia SC, Jeon YJ, Marquis C, Adesina A, Rogers PL (2013) Enzymatic conversion of coconut oil for biodiesel production. Fuel Process Technol 106:721–726CrossRefGoogle Scholar
  65. 65.
    Sonare NR, Rathod VK (2010) Transesterification of used sunflower oil using immobilized enzyme. J Mol Catal B Enzym 66:142–147CrossRefGoogle Scholar
  66. 66.
    Soumanoua MM, Bornscheuer UT (2003) Lipase-catalyzed alcoholysis of vegetable oils. Eur J Lipid Sci Technol 105:656–660CrossRefGoogle Scholar
  67. 67.
    Avhad MR, Marchetti JM (2015) A review on recent advancement in catalytic materials for biodiesel production. Renew Sustain Energy Rev 50:696–718CrossRefGoogle Scholar
  68. 68.
    Aguieiras ECG, Oliveira EDC, Freire DMG (2015) Current status and new developments of biodiesel production using fungal lipases. Fuel 159:52–67CrossRefGoogle Scholar
  69. 69.
    Jin Z, Han S, Zhang L, Zheng S, Wang Y, Lin Y (2013) Combined utilization of lipase displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media. Bioresour Technol 130:102–109CrossRefGoogle Scholar
  70. 70.
    Dizge N, Aydiner C, Imer DY, Bayramoglu M, Tanriseven A, Keskinler B (2009) Biodiesel production from sunflower, soybean, and waste cooking oils by transesterification using lipase immobilized onto a novel microporous polymer. Biores Technol 100:1983–1991CrossRefGoogle Scholar
  71. 71.
    Rodrigues AR, Paiva A, Silva MG, Simões P, Barreiros S (2011) Continuous enzymatic production of biodiesel from virgin and waste sunflower oil in supercritical carbon dioxide. J Supercrit Fluid 56:259–264CrossRefGoogle Scholar
  72. 72.
    Liu Y, Chen D, Xud X, Yand Y (2012) Evaluation of structure and hydrolysis activity of Candida rugosa Lip7 in presence of sub-/super-critical CO2. Enzyme Microbial Technol 51:354–358CrossRefGoogle Scholar
  73. 73.
    Dhake KP, Deshmukh KM, Patil YP, Singhal RS, Bhanage BM (2011) Improved activity and stability of Rhizopus oryzae lipase via immobilization for citronellol ester synthesis in supercritical carbon dioxide. J Biotechnol 156:46–51CrossRefGoogle Scholar
  74. 74.
    Silveira RL, Martínez J, Skaf MS, Martínez L (2012) Enzyme microheterogeneous hydration and stabilization in supercritical carbon dioxide. J Phys Chem B 116:5671–5678CrossRefGoogle Scholar
  75. 75.
    Badgujar KC, Dhake KP, Bhanage BM (2013) Immobilization of Candida cylindracea lipase on poly lactic acid, polyvinyl alcohol and chitosan based polymer film: characterization, activity, stability and its application for N-acylation reactions. Process Biochem 48:1335–1347CrossRefGoogle Scholar
  76. 76.
    Badgujar KC, Bhanage BM (2014) Synthesis of geranyl acetate in non-aqueous media using immobilized Pseudomonas cepacia lipase on biodegradable polymer film: kinetic modelling and chain length effect study. Process Biochem 49:1304–1313CrossRefGoogle Scholar
  77. 77.
    Badgujar VC, Badgujar KC, Yeole PM, Bhanage BM (2017) Immobilization of Rhizomucor miehei lipase on a polymeric film for synthesis of important fatty acid esters: kinetics and application studies. Bioprocess Biosyst Eng 40:1463–1478CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Vivek C. Badgujar
    • 1
    • 2
  • Kirtikumar C. Badgujar
    • 1
    • 3
  • Pravin M. Yeole
    • 4
  • Bhalchandra M. Bhanage
    • 1
  1. 1.Department of ChemistryInstitute of Chemical TechnologyMumbaiIndia
  2. 2.Department of ChemistryPratap College of Arts, Science and CommerceAmalnerIndia
  3. 3.Department of ChemistrySIES College of Arts, Science and CommerceMumbaiIndia
  4. 4.Department of ChemistryR. L. College of Arts and ScienceParolaIndia

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