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Bioprocess and Biosystems Engineering

, Volume 42, Issue 1, pp 37–46 | Cite as

Microbial calcium carbonate precipitation with high affinity to fill the concrete pore space: nanobiotechnological approach

  • Mostafa Seifan
  • Alireza Ebrahiminezhad
  • Younes Ghasemi
  • Aydin BerenjianEmail author
Research Paper
  • 104 Downloads

Abstract

Despite the advantages of concrete, it has a pore structure and is susceptible to cracking. The initiated cracks as well as pores and their connectivity accelerate the structure degradation by permitting aggressive substances to flow into the concrete matrix. This phenomenon results in a considerable repair and maintenance costs and decreases the concrete lifespan. In recent years, biotechnological approach through immobilization of bacteria in/or protective vehicles has emerged as a viable solution to address this issue. However, the addition of macro- or micro scale size particles can decrease the integrity of matrix. In this study, the immobilization of bacteria with magnetic iron oxide nanoparticle (ION) was proposed to protect the bacterial cell and evaluate their effect on healing the concrete pore space. The results show that the addition of immobilized bacteria with IONs resulted in a lower water absorption and volume of permeable pore space. Crystal analysis using scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS) revealed that CaCO3 was precipitated in bio-concrete specimen as a result of microbial biosynthesis.

Keywords

Iron oxide nanoparticle Immobilization CaCO3 Bacteria Concrete Water absorption 

Notes

Acknowledgements

This investigation was financially supported by The University of Waikato, New Zealand.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This study does not contain any studies with human participants or animals performed by any of the authors.

References

  1. 1.
    Van Tittelboom K, De Belie N, De Muynck W, Verstraete W (2010) Use of bacteria to repair cracks in concrete. Cem Concr Res 40:157–166CrossRefGoogle Scholar
  2. 2.
    Wiktor V, Jonkers HM (2011) Quantification of crack-healing in novel bacteria-based self-healing concrete. Cem Concr Compos 33:763–770CrossRefGoogle Scholar
  3. 3.
    Joseph C, Jefferson A, Cantoni M (2007) Issues relating to the autonomic healing of cementitious materials. In: First international conference on self-healing materials pp1-8 April 2007, Noordwijk aan Zee, The NetherlandsGoogle Scholar
  4. 4.
    Thao TDP, Johnson TJS, Tong QS, Dai PS (2009) Implementation of self-healing in concrete–proof of concept. IES J Part A Civ Struct Eng 2:116–125CrossRefGoogle Scholar
  5. 5.
    Dry C, McMillan W (1996) Three-part methylmethacrylate adhesive system as an internal delivery system for smart responsive concrete. Smart Mater Struct 5:297CrossRefGoogle Scholar
  6. 6.
    Dry CM (2000) Three designs for the internal release of sealants, adhesives, and waterproofing chemicals into concrete to reduce permeability. Cem Concr Res 30:1969–1977CrossRefGoogle Scholar
  7. 7.
    Huang H, Ye G, Leung C, Wan K (2011) Application of sodium silicate solution as self-healing agent in cementitious materials. In: International RILEM conference on advances in construction materials through science and engineering. RILEM Publications SARL: Hong Kong, China, pp 530–536Google Scholar
  8. 8.
    Seifan M, Samani AK, Burgess JJ, Berenjian A (2016) The effectiveness of microbial crack treatment in self healing concrete. In: Berenjian A, Jafarizadeh-Malmiri H, Song Y (eds) High Value Processing Technologies. Nova Science Publishers, Inc., New YorkGoogle Scholar
  9. 9.
    Wang JY, Soens H, Verstraete W, De Belie N (2014) Self-healing concrete by use of microencapsulated bacterial spores. Cem Concr Res 56:139–152CrossRefGoogle Scholar
  10. 10.
    Bang SS, Galinat JK, Ramakrishnan V (2001) Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii. Enzyme Microb Technol 28:404–409CrossRefGoogle Scholar
  11. 11.
    Wang J, Van Tittelboom K, De Belie N, Verstraete W (2012) Use of silica gel or polyurethane immobilized bacteria for self-healing concrete. Constr Build Mater 26:532–540CrossRefGoogle Scholar
  12. 12.
    Wang JY, De Belie N, Verstraete W (2012) Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete. J Ind Microbiol Biotechnol 39:567–577CrossRefGoogle Scholar
  13. 13.
    Wang JY, Snoeck D, Van Vlierberghe S, Verstraete W, De Belie N (2014) Application of hydrogel encapsulated carbonate precipitating bacteria for approaching a realistic self-healing in concrete. Constr Build Mater 68:110–119CrossRefGoogle Scholar
  14. 14.
    Land G, Stephan D (2015) Controlling cement hydration with nanoparticles. Cem Concr Compos 57:64–67CrossRefGoogle Scholar
  15. 15.
    Chen J, Kou S-c, Poon C-s (2012) Hydration and properties of nano-TiO2 blended cement composites. Cem Concr Compos 34:642–649CrossRefGoogle Scholar
  16. 16.
    Sato T, Beaudoin J (2011) Effect of nano-CaCO3 on hydration of cement containing supplementary cementitious materials. Adv Cement Res 23:33–43CrossRefGoogle Scholar
  17. 17.
    Oltulu M, Şahin R (2013) Effect of nano-SiO2, nano-Al2O3 and nano-Fe2O3 powders on compressive strengths and capillary water absorption of cement mortar containing fly ash: a comparative study. Energy Build 58:292–301CrossRefGoogle Scholar
  18. 18.
    Ji T (2005) Preliminary study on the water permeability and microstructure of concrete incorporating nano-SiO2. Cem Concr Res 35:1943–1947CrossRefGoogle Scholar
  19. 19.
    Seifan M, Ebrahiminezhad A, Ghasemi Y, Samani AK, Berenjian A (2018) Amine-modified magnetic iron oxide nanoparticle as a promising carrier for application in bio self-healing concrete. Appl Microbiol Biotechnol 102:175–184CrossRefGoogle Scholar
  20. 20.
    Seifan M, Ebrahiminezhad A, Ghasemi Y, Samani AK, Berenjian A (2018) The role of magnetic iron oxide nanoparticles in the bacterially induced calcium carbonate precipitation. Appl Microbiol Biotechnol 102:3595–3606CrossRefGoogle Scholar
  21. 21.
    Seifan M, Samani AK, Berenjian A (2016) Induced calcium carbonate precipitation using Bacillus species. Appl Microbiol Biotechnol 100:9895–9906CrossRefGoogle Scholar
  22. 22.
    Seifan M, Samani AK, Berenjian A (2017) New insights into the role of pH and aeration in the bacterial production of calcium carbonate (CaCO3). Appl Microbiol Biotechnol 101:3131–3142CrossRefGoogle Scholar
  23. 23.
    Seifan M, Samani AK, Berenjian A (2017) A novel approach to accelerate bacterially induced calcium carbonate precipitation using oxygen releasing compounds (ORCs). Biocatal Agri Biotechnol 12:299–307CrossRefGoogle Scholar
  24. 24.
    Seifan M, Samani AK, Hewitt S, Berenjian A (2017) The effect of cell immobilization by calcium alginate on bacterially induced calcium carbonate precipitation. Fermentation 3:57CrossRefGoogle Scholar
  25. 25.
    Ebrahiminezhad A, Davaran S, Rasoul-Amini S, Barar J, Moghadam M, Ghasemi Y (2012) Synthesis, characterization and anti-listeria monocytogenes effect of amino acid coated magnetite nanoparticles. Curr Nanosci 8:868–874CrossRefGoogle Scholar
  26. 26.
    Ebrahiminezhad A, Ghasemi Y, Rasoul-Amini S, Barar J, Davaran S (2012) Impact of amino-acid coating on the synthesis and characteristics of iron-oxide nanoparticles (IONs). B Kor Chem Soc 33:3957–3962CrossRefGoogle Scholar
  27. 27.
    Ebrahiminezhad A, Ghasemi Y, Rasoul-Amini S, Barar J, Davaran S (2013) Preparation of novel magnetic fluorescent nanoparticles using amino acids. Colloids Surf B 102:534–539CrossRefGoogle Scholar
  28. 28.
    Ranmadugala D, Ebrahiminezhad A, Manley-Harris M, Ghasemi Y, Berenjian A (2017) The effect of iron oxide nanoparticles on Bacillus subtilis biofilm, growth and viability. Process Biochem 62:231–240CrossRefGoogle Scholar
  29. 29.
    Wu W, He Q, Jiang C (2008) Magnetic iron oxide nanoparticles: Synthesis and surface functionalization strategies. Nanoscale Res Lett 3:397–415CrossRefGoogle Scholar
  30. 30.
    Seifan M, Sarmah AK, Samani AK, Ebrahiminezhad A, Ghasemi Y, Berenjian A (2018) Mechanical properties of bio self-healing concrete containing immobilized bacteria with iron oxide nanoparticles. Appl Microbiol Biotechnol 102:4489–4498CrossRefGoogle Scholar
  31. 31.
    ASTM C642-97 (1997) Standard test method for density, absorption, and voids in hardened concrete. American Society of Testing Materials. ASTM International, West ConshohockenGoogle Scholar
  32. 32.
    Ebrahiminezhad A, Taghizadeh S, Ghasemi Y, Berenjian A (2018) Green synthesized nanoclusters of ultra-small zero valent iron nanoparticles as a novel dye removing material. Sci Total Environ 621:1527–1532CrossRefGoogle Scholar
  33. 33.
    Sanchez F, Sobolev K (2010) Nanotechnology in concrete—a review. Constr Build Mater 24:2060–2071CrossRefGoogle Scholar
  34. 34.
    Jo BW, Kim CH, Tae GH, Park JB (2007) Characteristics of cement mortar with nano-SiO2 particles. Const Build Mater 21:1351–1355CrossRefGoogle Scholar
  35. 35.
    Qing Y, Zenan Z, Deyu K, Rongshen C (2007) Influence of nano-SiO2 addition on properties of hardened cement paste as compared with silica fume. Constr Build Mater 21:539–545CrossRefGoogle Scholar
  36. 36.
    Jayapalan A, Lee B, Fredrich S, Kurtis K (2010) Influence of additions of anatase TiO2 nanoparticles on early-age properties of cement-based materials. Transp Res Rec 41–46Google Scholar
  37. 37.
    Li H, Zhang Mh, Ou Jp (2006) Abrasion resistance of concrete containing nano-particles for pavement. Wear 260:1262–1266CrossRefGoogle Scholar
  38. 38.
    Sato T, Diallo F (2010) Seeding effect of nano-CaCO3 on the hydration of tricalcium silicate. Transp Res Rec 61–67Google Scholar
  39. 39.
    Li H, Xiao HG, Ou JP (2004) A study on mechanical and pressure-sensitive properties of cement mortar with nanophase materials. Cem Concr Res 34:435–438CrossRefGoogle Scholar
  40. 40.
    Li Z, Wang H, He S, Lu Y, Wang M (2006) Investigations on the preparation and mechanical properties of the nano-alumina reinforced cement composite. Mater Lett 60:356–359CrossRefGoogle Scholar
  41. 41.
    Chang T-P, Shih J-Y, Yang K-M, Hsiao T-C (2007) Material properties of Portland cement paste with nano-montmorillonite. J Mater Sci 42:7478–7487CrossRefGoogle Scholar
  42. 42.
    Phoo-ngernkham T, Chindaprasirt P, Sata V, Hanjitsuwan S, Hatanaka S (2014) The effect of adding nano-SiO2 and nano-Al2O3 on properties of high calcium fly ash geopolymer cured at ambient temperature. Mater Design 55:58–65CrossRefGoogle Scholar
  43. 43.
    Nazari A, Riahi S, Riahi S, Shamekhi SF, Khademno A (2010) Influence of Al2O3 nanoparticles on the compressive strength and workability of blended concrete. J Am Sci 6:6–9Google Scholar
  44. 44.
    Ebrahiminezhad A, Varma V, Yang S, Ghasemi Y, Berenjian A (2015) Synthesis and application of amine functionalized iron oxide nanoparticles on menaquinone-7 fermentation: a step towards process intensification. Nanomaterials 6:1–9CrossRefGoogle Scholar
  45. 45.
    Ebrahiminezhad A, Bagheri M, Taghizadeh SM, Berenjian A, Ghasemi Y (2016) Biomimetic synthesis of silver nanoparticles using microalgal secretory carbohydrates as a novel anticancer and antimicrobial. Adv Nat Sci-Nanosci 7Google Scholar
  46. 46.
    Gupta AK, Gupta M (2005) Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26:3995–4021CrossRefGoogle Scholar
  47. 47.
    Assa F, Jafarizadeh-Malmiri H, Ajamein H, Anarjan N, Vaghari H, Sayyar Z, Berenjian A (2016) A biotechnological perspective on the application of iron oxide nanoparticles. Nano Res 9:2203–2225CrossRefGoogle Scholar
  48. 48.
    Seifan M, Sarmah K, Ebrahiminezhad A, Ghasemi A, Samani Y, Berenjian AK A (2018) Bio-reinforced self-healing concrete using magnetic iron oxide nanoparticles. Appl Microbiol Biotechnol 102:2167–2178CrossRefGoogle Scholar
  49. 49.
    Qiao R, Yang C, Gao M (2009) Superparamagnetic iron oxide nanoparticles: from preparations to in vivo MRI applications. J Mater Chem 19:6274–6293CrossRefGoogle Scholar
  50. 50.
    Levy L, Sahoo Y, Kim KS, Bergey EJ, Prasad PN (2002) Nanochemistry: synthesis and characterization of multifunctional nanoclinics for biological applications. Chem Mater 14:3715–3721CrossRefGoogle Scholar
  51. 51.
    Wang J, Deng T, Dai Y (2005) Study on the processes and mechanism of the formation of Fe3O4 at low temperature. J Alloys Compd 390:127–132CrossRefGoogle Scholar
  52. 52.
    Mahdavi M, Ahmad MB, Haron MJ, Namvar F, Nadi B, Ab Rahman MZ, Amin J (2013) Synthesis, surface modification and characterisation of biocompatible magnetic iron oxide nanoparticles for biomedical applications. Molecules 18:7533–7548CrossRefGoogle Scholar
  53. 53.
    Sun J, Zhou S, Hou P, Yang Y, Weng J, Li X, Li M (2007) Synthesis and characterization of biocompatible Fe3O4 nanoparticles. J Biomed Mater Res A 80:333–341CrossRefGoogle Scholar
  54. 54.
    Fujita Y, Ferris FG, Lawson RD, Colwell FS, Smith RW (2000) Calcium carbonate precipitation by ureolytic subsurface bacteria. Geomicrobiol J 17:305–318CrossRefGoogle Scholar
  55. 55.
    Panda R, Gajbhiye N, Balaji G (2001) Magnetic properties of interacting single domain Fe3O4 particles. J Alloys Compd 326:50–53CrossRefGoogle Scholar
  56. 56.
    Goya G, Berquo T, Fonseca F, Morales M (2003) Static and dynamic magnetic properties of spherical magnetite nanoparticles. J Appl Phys 94:3520–3528CrossRefGoogle Scholar
  57. 57.
    de Montferrand C, Hu L, Milosevic I, Russier V, Bonnin D, Motte L, Brioude A, Lalatonne Y (2013) Iron oxide nanoparticles with sizes, shapes and compositions resulting in different magnetization signatures as potential labels for multiparametric detection. Acta Biomater 9:6150–6157CrossRefGoogle Scholar
  58. 58.
    Peternele WS, Fuentes VM, Fascineli ML, Silva JRd, Silva RC, Lucci CM, Azevedo RBd (2014) Experimental investigation of the coprecipitation method: an approach to obtain magnetite and maghemite nanoparticles with improved properties. J Nanomater 94Google Scholar
  59. 59.
    Ebrahiminezhad A, Varma V, Yang S, Berenjian A (2016) Magnetic immobilization of Bacillus subtilis natto cells for menaquinone-7 fermentation. Appl Microbiol Biotechnol 100:173–180CrossRefGoogle Scholar
  60. 60.
    Kourkoutas Y, Bekatorou A, Banat IM, Marchant R, Koutinas AA (2004) Immobilization technologies and support materials suitable in alcohol beverages production: a review. Food Microbiol 21:377–397CrossRefGoogle Scholar
  61. 61.
    Castro J, Bentz D, Weiss J (2011) Effect of sample conditioning on the water absorption of concrete. Cem Concr Compos 33:805–813CrossRefGoogle Scholar
  62. 62.
    Burne RA, Chen YYM (2000) Bacterial ureases in infectious diseases. Microbes Infect 2:533–542CrossRefGoogle Scholar
  63. 63.
    Seifan M, Samani AK, Berenjian A (2016) Bioconcrete: next generation of self-healing concrete. Appl Microbiol Biotechnol 100:2591–2602CrossRefGoogle Scholar
  64. 64.
    Kim HK, Park SJ, Han JI, Lee HK (2013) Microbially mediated calcium carbonate precipitation on normal and lightweight concrete. Constr Build Mater 38:1073–1082CrossRefGoogle Scholar
  65. 65.
    Wang J, Dewanckele J, Cnudde V, Van Vlierberghe S, Verstraete W, De Belie N (2014) X-ray computed tomography proof of bacterial-based self-healing in concrete. Cem Concr Compos 53:289–304CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mostafa Seifan
    • 1
  • Alireza Ebrahiminezhad
    • 2
  • Younes Ghasemi
    • 2
    • 3
  • Aydin Berenjian
    • 1
    Email author
  1. 1.School of Engineering, Faculty of Science and EngineeringThe University of WaikatoHamiltonNew Zealand
  2. 2.Department of Medical Nanotechnology, School of Advanced Medical Sciences and TechnologiesShiraz University of Medical ScienceShirazIran
  3. 3.Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research CentreShiraz University of Medical SciencesShirazIran

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