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Bioprocess and Biosystems Engineering

, Volume 41, Issue 11, pp 1697–1706 | Cite as

Kinetic analysis of sodium gluconate production by Aspergillus niger with different inlet oxygen concentrations

  • Xiwei Tian
  • Yuting Shen
  • Yingping Zhuang
  • Wei Zhao
  • Haifeng Hang
  • Ju Chu
Research Paper
  • 153 Downloads

Abstract

To further understand fermentation kinetics of sodium gluconate (SG) production by Aspergillus niger with different inlet oxygen concentrations, logistic model for cell growth and two-step models for SG production and glucose consumption were established. The results demonstrated that the maximum specific growth rate (µm) presented exponential relationship with inlet oxygen concentration and the maximum biomass (Xm) exhibited linear increase. In terms of SG production, two-step model with Luedeking–Piret equation during growth phase and oxygen-dependent equation during stationary phase could well fit the experimental data. Notably, high inlet oxygen concentration exponentially improved SG yield (YP/S), whereas biomass yield to glucose (YX/S) and cell maintenance coefficient (m) were almost independent on inlet oxygen concentration, indicating that high oxygen supply enhancing SG synthesis not only functioning as a substrate directly, but also regulating glucose metabolism towards SG formation. Finally, the applicability and predictability of the proposed models were further validated by additional experiments.

Keywords

Aspergillus niger Sodium gluconate Oxygen Kinetics 

Notes

Acknowledgements

This work was financially supported by the National Science Foundation for Young Scientists of China (31700038), the National Key Research and Development Program (2017YFB0309302), and the Fundamental Research Funds for the Central Universities (WF1814032, 22221818014).

Compliance with ethical standards

Conflict of interest

The authors declared that they have no competing interests.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiPeople’s Republic of China
  2. 2.Shan Dong Fuyang Biological Technology Co., ltdDezhouChina

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