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Separation and purification of α-ketoglutarate and pyruvate from the fermentation broth of Yarrowia lipolytica

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A strategy to achieve the efficient co-production of α-ketoglutarate (KGA) and pyruvate (PYR) via Yarrowia lipolytica fermentation was established in our previous work. The next big challenge is to achieve an efficient separation of the two keto acids. A strategy for simultaneously separating and purifying KGA and PYR based on their different boiling points was established, leading to the efficient separation and purification of the two keto acids from the fermentation broth of Y. lipolytica. The purity and yield of KGA/PYR reached 99.3/99.5 and 79.8/80.6%, respectively. Application of the separation method on industrial scale could further decrease the cost of the production of the two keto acids by biotechnological routes.

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This work was supported by the National Key Research and Development Program of China (2017YFC1600403), the National Natural Science Foundation of China (Major Program, 21390204), the Key Research and Development Program of Jiangsu Province (BE2016689), the National Natural Science Foundation of China (31670095, 31770097), the Fundamental Research Funds for the Central Universities (JUSRP51701A), the Distinguished Professor Project of Jiangsu Province, the National First-class Discipline Program of Light Industry Technology and Engineering (LITE2018-08), and the 111 Project (111-2-06).

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Correspondence to Jingwen Zhou.

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Zeng, W., Xu, S., Du, G. et al. Separation and purification of α-ketoglutarate and pyruvate from the fermentation broth of Yarrowia lipolytica. Bioprocess Biosyst Eng 41, 1519–1527 (2018). https://doi.org/10.1007/s00449-018-1979-z

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  • Keto acids
  • Yarrowia lipolytica
  • Ethyl acetate
  • Extraction
  • Purification