Enhanced production of succinic acid from methanol–organosolv pretreated Strophanthus preussii by recombinant Escherichia coli
- 94 Downloads
A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem been addressed is utilization of waste biomass for the production of succinic acid using metabolic engineering strategy. Here, methanol extract of Strophanthus preussii was used for fermentation. The process parameters were optimized. Glucose (9 g/L), galactose (4 g/L), xylose (6 g/L) and arabinose (0.5 g/L) were the major sugars present in the methanol extract of S. preussii. E. coli K3OS with overexpression of soluble nucleotide pyridine transhydrogenase sthA and mutation of lactate dehydrogenase A (ldhA), phosphotransacetylase acetate kinase A (pta-ackA), pyruvate formate lyase B (pflB), pyruvate oxidase B (poxB), produced a final succinic acid concentration of 14.40 g/L and yield of 1.10 mol/mol total sugars after 72 h dual-phase fermentation in M9 medium. Here, we show that the maximum theoretical yield using methanol extracts of S. preussii was 64%. Hence, methanol extract of S. preussii could be used for the production of biochemicals such as succinate, malate and pyruvate.
KeywordsEscherichia coli Succinic acid Fermentation Strophanthus preussii Biorefinery
This work was supported by National High Technology Research and Development Program of China (863 Project, No 2014AA021905).
- 7.Ichwan M, Son T (2011) Study on organosolv pulping methods of oil palm biomass. In: Proceedings of the 2nd international seminar on chemistry 2011, JatinangorGoogle Scholar
- 17.Cheng KK, Wang GY, Zeng J, Zhang JA (2013) Improved succinate production by metabolic engineering. Biomed Res Int 538790:1–12Google Scholar
- 21.Jantama K, Polyiam P, Khunonkawo P, Chan S, Sangproo M, Khor K, Jantama SS, Kanchanatawee S (2015) Efficient reduction of the formation of by-products and improvement of production yield of 2,3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically engineered Klebsiella oxytoca in mineral salts medium. Metab Eng 30:16–26CrossRefPubMedGoogle Scholar
- 28.Yokoyama S, Matsumura Y (2008) The Asian biomass handbook. The Japan institute of energy, p 101Google Scholar
- 29.Simonyan K, Fasina O (2013) Biomass resources and bioenergy potentials in Nigeria. Afr J Agric Res 8:4975–4989Google Scholar
- 31.Sambrook JR, Russel D (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, p 1839Google Scholar
- 32.Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008) Determination of ash in biomass. Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory, GoldenGoogle Scholar
- 33.Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass. Lab Anal Proced 1617:1–16Google Scholar
- 36.Russell JB, Cook GM (1995) Energetics of bacterial growth: balance of anabolic and catabolic reactions. Microb Rev 59:48–62Google Scholar
- 40.Fraenkel D, Levisohn S (1967) Glucose and gluconate metabolism in an Escherichia coli mutant lacking phosphoglucose isomerase. J Bacteriol 93:571–1578Google Scholar
- 43.Lin E, Kuritzkes D (1987) Pathways for anaerobic electron transport. Escherichia coli and Salmonella typhimurium: cellular and molecular biology. Am Soc Microbiol 1:201–221Google Scholar