Enhanced production of succinic acid from methanol–organosolv pretreated Strophanthus preussii by recombinant Escherichia coli
- 131 Downloads
Abstract
A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem been addressed is utilization of waste biomass for the production of succinic acid using metabolic engineering strategy. Here, methanol extract of Strophanthus preussii was used for fermentation. The process parameters were optimized. Glucose (9 g/L), galactose (4 g/L), xylose (6 g/L) and arabinose (0.5 g/L) were the major sugars present in the methanol extract of S. preussii. E. coli K3OS with overexpression of soluble nucleotide pyridine transhydrogenase sthA and mutation of lactate dehydrogenase A (ldhA), phosphotransacetylase acetate kinase A (pta-ackA), pyruvate formate lyase B (pflB), pyruvate oxidase B (poxB), produced a final succinic acid concentration of 14.40 g/L and yield of 1.10 mol/mol total sugars after 72 h dual-phase fermentation in M9 medium. Here, we show that the maximum theoretical yield using methanol extracts of S. preussii was 64%. Hence, methanol extract of S. preussii could be used for the production of biochemicals such as succinate, malate and pyruvate.
Keywords
Escherichia coli Succinic acid Fermentation Strophanthus preussii BiorefineryNotes
Acknowledgements
This work was supported by National High Technology Research and Development Program of China (863 Project, No 2014AA021905).
Supplementary material
References
- 1.McKinlay JB, Vieille C, Zeikus JG (2007) Prospects for a bio-based succinate industry. Appl Microbiol Biotechnol 76:727–740CrossRefPubMedGoogle Scholar
- 2.Bradfield MFA, Mohagheghi A, Salvachúa D, Smith H, Black BA, Dowe N, Beckham GT, Nicol W (2015) Continuous succinic acid production by Actinobacillus succinogenes on xylose-enriched hydrolysate. Biotechnol Biofuels 8:1–6CrossRefGoogle Scholar
- 3.Ball GL, McLellan CJ, Bhat VS (2012) Toxicological review and oral risk assessment of terephthalic acid (TPA) and its esters: a category approach. Crit Rev Toxicol 42:28–67CrossRefPubMedGoogle Scholar
- 4.Stols L, Donnelly MI (1997) Production of succinic acid through overexpression of NAD (+)-dependent malic enzyme in an Escherichia coli mutant. Appl Environ Microbiol 63:2695–2701PubMedPubMedCentralGoogle Scholar
- 5.Olajuyin AM, Yang M, Liu Y, Mu T, Tian J, Adaramoye OA, Xing J (2016) Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli. Bioresour Technol 214:653–659CrossRefPubMedGoogle Scholar
- 6.Chen MY, Ike M, Fujita M (2002) Acute toxicity, mutagenicity, and estrogenicity of bisphenol-A and other bisphenols. Environ Toxicol 17:80–86CrossRefPubMedGoogle Scholar
- 7.Ichwan M, Son T (2011) Study on organosolv pulping methods of oil palm biomass. In: Proceedings of the 2nd international seminar on chemistry 2011, JatinangorGoogle Scholar
- 8.Bozell JJ, Petersen GR (2010) Technology development for the production of biobased products from biorefinery carbohydrates—the US Department of Energy’s “top 10” revisited. Green Chem 12:539–554CrossRefGoogle Scholar
- 9.Salvachúa D, Mohagheghi A, Smith H, Bradfield MFA, Nicol WB, Black A, Biddy MJ, Dowe N, Beckha GT (2016) Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation. Biotechnol Biofuel 9:1–6CrossRefGoogle Scholar
- 10.Zhao X, Cheng K, Liu D (2009) Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis. Appl Microbiol Biotechnol 82:815–827CrossRefPubMedGoogle Scholar
- 11.Kumar P, Barrett DM, Delwiche MJ, Stroeve P (2009) Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Ind Eng Chem Res 48:3713–3729CrossRefGoogle Scholar
- 12.Chandra RP, Bura R, Mabee WE, Berlin A, Pan X, Saddler JN (2007) Substrate pretreatment: the key to effective enzymatic hydrolysis of lignocellulosics. Adv Biochem Eng Biotechnol 108:67–93PubMedGoogle Scholar
- 13.Jantama K, Zhang X, Moore JC, Shanmugam KT, Svoronos KT, Ingram LO (2008) Eliminating side products and increasing succinate yields in engineered strains of Escherichia coli C. Biotechnol Bioeng 101:881–893CrossRefPubMedGoogle Scholar
- 14.Lee SJ, Lee DY, Kim TY, Kim BH, Lee J (2005) Metabolic engineering of Escherichia coli for enhanced production of succinic acid, based on genome comparison and in silico gene knockout simulation. Appl Environ Microbiol 71:7880–7887CrossRefPubMedPubMedCentralGoogle Scholar
- 15.Mienda BS, Shamsir MS, Illias RMD (2016) Model-assisted formate dehydrogenase-O (fdoH) gene knockout for enhanced succinate production in Escherichia coli from glucose and glycerol carbon sources. J Biomol Struct Dyn 34:2305–2316CrossRefPubMedGoogle Scholar
- 16.Mienda BS, Shamsir MS, Illias RM (2016) Model-guided metabolic gene knockout of gnd for enhanced succinate production in Escherichia coli from glucose and glycerol substrates. Comput Biol Chem 61:130–137CrossRefPubMedGoogle Scholar
- 17.Cheng KK, Wang GY, Zeng J, Zhang JA (2013) Improved succinate production by metabolic engineering. Biomed Res Int 538790:1–12Google Scholar
- 18.Mienda BS, Salleh FM (2017) Bio-succinic acid production: Escherichia coli strains design from genome-scale perspectives. AIMS Bioeng 4:418–430CrossRefGoogle Scholar
- 19.Bai B, Zhou JM, Yang MH, Liu YL, Xu XH, Xing JM (2015) Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli. Bioresour Technol 185:56–61CrossRefPubMedGoogle Scholar
- 20.Chan S, Kanchanatawee S, Jantama K (2012) Production of succinic acid from sucrose and sugarcane molasses by metabolically engineered Escherichia coli. Bioresour Technol 103:329–33621CrossRefPubMedGoogle Scholar
- 21.Jantama K, Polyiam P, Khunonkawo P, Chan S, Sangproo M, Khor K, Jantama SS, Kanchanatawee S (2015) Efficient reduction of the formation of by-products and improvement of production yield of 2,3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically engineered Klebsiella oxytoca in mineral salts medium. Metab Eng 30:16–26CrossRefPubMedGoogle Scholar
- 22.Lee SJ, Song H, Lee SY (2006) Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production. Appl Environ Microbiol 72:1939–1948CrossRefPubMedPubMedCentralGoogle Scholar
- 23.Liang L, Liu R, Wang G, Gou D, Ma J, Chen K (2012) Regulation of NAD(H) pool and NADH/NAD(+) ratio by overexpression of nicotinic acid phosphoribosyltransferase for succinic acid production in Escherichia coli NZN111. Enzym Microb Technol 51:286–293CrossRefGoogle Scholar
- 24.Sánchez AM, Bennett GN, San KY (2005) Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity. Metab Eng 7:229–239CrossRefPubMedGoogle Scholar
- 25.Sauer M, Porro D, Mattanovich D, Branduardi P (2008) Microbial production of organic acids: expanding the markets. Trends Biotechnol 26:100–108CrossRefPubMedGoogle Scholar
- 26.Nyawuame H, Gill L (1991) Cuticular studies of some West African species of the Apocynaceae of medicinal value. Feddes Repert 102:87–104CrossRefGoogle Scholar
- 27.Osseo-Asare AD (2008) Bioprospecting and resistance: transforming poisoned arrows into Strophantin Pills in Colonial Gold Coast, 1885–1922. Soc His Med 21:269–290CrossRefGoogle Scholar
- 28.Yokoyama S, Matsumura Y (2008) The Asian biomass handbook. The Japan institute of energy, p 101Google Scholar
- 29.Simonyan K, Fasina O (2013) Biomass resources and bioenergy potentials in Nigeria. Afr J Agric Res 8:4975–4989Google Scholar
- 30.Liu Y, Yang M, Chen J, Yan D, Cheng W, Wang Y, Thygesen A, Chen R, Xing J, Wang Q, Ma Y (2016) PCR-based seamless genome editing with high efficiency and fidelity in Escherichia coli. PLoS ONE 11:e0149762CrossRefPubMedPubMedCentralGoogle Scholar
- 31.Sambrook JR, Russel D (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, p 1839Google Scholar
- 32.Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008) Determination of ash in biomass. Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory, GoldenGoogle Scholar
- 33.Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass. Lab Anal Proced 1617:1–16Google Scholar
- 34.Bai B, Zhou J, Yang M, Liu Y, Xu X, Xing J (2015) Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli. Bioresour Technol 185:56–61CrossRefPubMedGoogle Scholar
- 35.Singh A, Soh KC, Hatzimanikatis V, Gill RT (2011) Manipulating redox and ATP balancing for improved production of succinate in E. coli. Metab Eng 13:76–81CrossRefPubMedGoogle Scholar
- 36.Russell JB, Cook GM (1995) Energetics of bacterial growth: balance of anabolic and catabolic reactions. Microb Rev 59:48–62Google Scholar
- 37.Liu R, Liang L, Li F, Wu M, Chen K, Ma J (2013) Efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered Escherichia coli. Bioresour Technol 149:84–91CrossRefPubMedGoogle Scholar
- 38.Wang D, Li Q, Maohua Y, Zhang Y, Su Z, Xing J (2011) Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation. Process Biochem 46:365–371CrossRefGoogle Scholar
- 39.Steinsiek S, Bettenbrock K (2012) Glucose transport in Escherichia coli mutant strains with defects in sugar transport systems. J Bacteriol 194:5897–5908CrossRefPubMedPubMedCentralGoogle Scholar
- 40.Fraenkel D, Levisohn S (1967) Glucose and gluconate metabolism in an Escherichia coli mutant lacking phosphoglucose isomerase. J Bacteriol 93:571–1578Google Scholar
- 41.Zhang X, Jantama K, Moore JC, Jarboe LR, Shanmugam KT, Ingrama LO (2009) Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli. Proc Natl Acad Sci USA 106:20180–20185CrossRefPubMedGoogle Scholar
- 42.Sprenger GA (1995) Genetics of pentose-phosphate pathway enzymes of Escherichia coli K-12. Arch Microbiol 164:324–330CrossRefPubMedGoogle Scholar
- 43.Lin E, Kuritzkes D (1987) Pathways for anaerobic electron transport. Escherichia coli and Salmonella typhimurium: cellular and molecular biology. Am Soc Microbiol 1:201–221Google Scholar
- 44.Saler MH, Reizer J (1994) The bacterial phosphotransferase system: new frontiers 30 years later. Mol Microbiol 13:755–764CrossRefGoogle Scholar
- 45.Fong SS, Palsson BO (2004) Metabolic gene-deletion strains of Escherichia coli evolve to computationally predicted growth phenotypes. Nat Genet 36:1056–1058CrossRefPubMedGoogle Scholar
- 46.Müller-Hartmann H, Müller-Hill B (1996) The side-chain of the amino acid residue in position 110 of the Lac repressor influences its allosteric equilibrium. J Mol Biol 257:473–478CrossRefPubMedGoogle Scholar
- 47.Vemuri GN, Eiteman MA, Altman E (2002) Effects of growth mode and pyruvate carboxylase on succinic acid production by metabolically engineered strains of Escherichia coli. Appl Environ Microbiol 68:1715–1727CrossRefPubMedPubMedCentralGoogle Scholar
- 48.Canonaco F, Hess TA, Heri S, Wang T, Szyperski T, Sauer U (2001) Metabolic flux response to phosphoglucose isomerase knock-out in Escherichia coli and impact of overexpression of the soluble transhydrogenase UdhA. FEMS Microbiol Lett 204:247–252CrossRefPubMedGoogle Scholar