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Bioprocess and Biosystems Engineering

, Volume 41, Issue 8, pp 1195–1204 | Cite as

Butyrate-based n-butanol production from an engineered Shewanella oneidensis MR-1

  • Jong-Min Jeon
  • Hun-Suk Song
  • Doo-Geun Lee
  • Ju Won Hong
  • Yoon Gi Hong
  • Yu-Mi Moon
  • Shashi Kant Bhatia
  • Jeong-Jun Yoon
  • Wooseong Kim
  • Yung-Hun Yang
Research Paper
  • 94 Downloads

Abstract

n-Butanol is considered as the next-generation biofuel, because its physiochemical properties are very similar to fossil fuels and it could be produced by Clostridia under anaerobic culture. Due to the difficulties of strict anaerobic culture, a host which can be used with facultative environment was being searched for n-butanol production. As an alternative, Shewanella oneidensis MR-1, which is known as facultative bacteria, was selected as a host and studied. A plasmid containing adhE2 encoding alcohol dehydrogenase, various CoA transferases (ctfAB, atoAD, pct, and ACT), and acs encoding acetyl-CoA synthetase were introduced and examined to S. oneidensis MR-1 to produce n-butanol. As a result, ctfAB, acs, and adhE2 overexpression in S. oneidensis-pJM102 showed the highest n-butanol production in the presence of 2% of N-acetylglucosamine (NAG), 0.3% of butyrate, and 0.1 mM of IPTG for 96 h under microaerobic condition. When more NAG and butyrate were fed, n-butanol production was enhanced, producing up to 160 mg/L of n-butanol. When metal ions or extra electrons were added to S. oneidensis-pJM102 for n-butanol production, metal ion as electron acceptor or supply of extra electron showed no significant effect on n-butanol production. Overall, we made a newly engineered S. oneidensis that could utilize NAG and butyrate to produce n-butanol. It could be used in further microaerobic condition and electricity supply studies.

Keywords

n-Butanol Shewanella oneidensis MR-1 Electron transfer 

Notes

Acknowledgements

This study was also supported by the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2015M1A5A1037196, NRF2016R1D1A1B03932301), Research Program to solve social issues of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017M3A9E4077234), and Korea Institute of Energy Technology Evaluation and Planning (KETEP), and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (No. 20163010092150). Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, Korea) was kindly appreciated.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jong-Min Jeon
    • 1
  • Hun-Suk Song
    • 1
  • Doo-Geun Lee
    • 2
  • Ju Won Hong
    • 1
  • Yoon Gi Hong
    • 1
  • Yu-Mi Moon
    • 1
  • Shashi Kant Bhatia
    • 1
  • Jeong-Jun Yoon
    • 2
  • Wooseong Kim
    • 3
  • Yung-Hun Yang
    • 1
    • 4
  1. 1.Department of Biological Engineering, College of EngineeringKonkuk UniversitySeoulSouth Korea
  2. 2.Intelligent Sustainable Materials R&D GroupKorea Institute of Industrial Technology (KITECH)Chonan-siSouth Korea
  3. 3.Division of Infectious Disease, Rhode Island HospitalAlpert Medical School of Brown UniversityProvidenceUSA
  4. 4.Institute for Ubiquitous Information Technology and Applications (CBRU)Konkuk UniversitySeoulSouth Korea

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