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Bioprocess and Biosystems Engineering

, Volume 41, Issue 8, pp 1133–1142 | Cite as

Enhancing enzyme-aided production of fermentable sugars from poplar pulp in the presence of non-ionic surfactants

  • A. Alhammad
  • P. Adewale
  • M. Kuttiraja
  • L. P. Christopher
Research Paper
  • 67 Downloads

Abstract

Addition of surfactants to enzymatic hydrolysis has been reported to enhance the hydrolytic potential of enzymes in the bioconversion of lignocellulosic biomass to fermentable sugars. The objective of this investigation was to evaluate the effects of four non-ionic surfactants (PEG4000, PEG8000, TitronX-100, and Tween80) on the efficiency of enzymatic hydrolysis of steam-pretreated poplar using a commercial cellulase preparation (Cellic® CTec2). Statistical discriminant analysis at four variable factors (surfactant type, surfactant concentration, hydrolysis time, and substrate consistency) revealed that enzymatic hydrolysis was significantly enhanced in the presence of PEG4000, with 19.2% increase in glucose yield over control without surfactant, whereas ANOVA test indicated substrate consistency and hydrolysis time as the most significant factors (P < 0.05). Hydrolysis of poplar pulp at 5% w/w pulp consistency with CTec2 in presence of 1% w/w PEG4000 produced the highest glucose yield of 58.5% after 96 h reaction time.

Keywords

Enzymatic hydrolysis Cellulase Poplar pulp Non-ionic surfactants Fermentable sugars Discriminant analysis 

Notes

Acknowledgements

Financial support by the Biorefining Research Institute at Lakehead University is gratefully acknowledged.

References

  1. 1.
    Singhvi MS, Chaudhari S, Gokhale DV (2014) Lignocellulose processing: a current challenge. RSC Adv 4:8271–8277CrossRefGoogle Scholar
  2. 2.
    Wojtusik M, Zurita M, Villar JC, Ladero M, Garcia-Ochoa F (2016) Influence of fluid dynamic conditions on enzymatic hydrolysis of lignocellulosic biomass: effect of mass transfer rate. Bioresour Technol 216:28–35CrossRefPubMedGoogle Scholar
  3. 3.
    Sun S, Sun S, Cao X, Sun R (2016) The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials. Bioresour Technol 199:49–58CrossRefPubMedGoogle Scholar
  4. 4.
    Liu W, Wang B, Hou Q, Chen W, Wu M (2016) Effects of fibrillation on the wood fibers’ enzymatic hydrolysis enhanced by mechanical refining. Bioresour Technol 206:99–103CrossRefPubMedGoogle Scholar
  5. 5.
    Wang ZJ, Zhu JY, Zalesny RS, Chen KF (2012) Ethanol production from poplar wood through enzymatic saccharification and fermentation by dilute acid and SPORL pretreatments. Fuel 95:606–614CrossRefGoogle Scholar
  6. 6.
    Saini JK, Patel AK, Adsul M, Singhania RR (2016) Cellulase adsorption on lignin: A roadblock for economic hydrolysis of biomass. Renew Energy 98:29–42CrossRefGoogle Scholar
  7. 7.
    Chang K-L, Chen X-M, Wang X-Q, Han Y-J, Potprommanee L, Liu J-Y, Liao Y-L, Ning X-A, Sun S-Y, Huang Q (2017) Impact of surfactant type for ionic liquid pretreatment on enhancing delignification of rice straw. Bioresour Technol 227:388–392CrossRefPubMedGoogle Scholar
  8. 8.
    Neto JM, dos Reis Garcia D, Rueda SMG, da Costa AC (2013) Study of kinetic parameters in a mechanistic model for enzymatic hydrolysis of sugarcane bagasse subjected to different pretreatments. Bioproc Biosyst Eng 36:1579–1590CrossRefGoogle Scholar
  9. 9.
    Meng X, Sun Q, Kosa M, Huang F, Pu Y, Ragauskas AJ (2016) Physicochemical structural changes of poplar and switchgrass during biomass pretreatment and enzymatic hydrolysis. ACS Sustain Chem Eng 4:4563–4572CrossRefGoogle Scholar
  10. 10.
    Pihlajaniemi V, Sipponen MH, Kallioinen A, Nyyssölä A, Laakso S (2016) Rate-constraining changes in surface properties, porosity and hydrolysis kinetics of lignocellulose in the course of enzymatic saccharification. Biotechnol Biofuels 9:18CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Seo D-J, Fujita H, Sakoda A (2011) Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification. Bioresour Technol 102:9605–9612CrossRefPubMedGoogle Scholar
  12. 12.
    Torr KM, Love KT, Simmons BA, Hill SJ (2016) Structural features affecting the enzymatic digestibility of pine wood pretreated with ionic liquids. Biotechnol Bioeng 113:540–549CrossRefPubMedGoogle Scholar
  13. 13.
    Chu Q, Chandra RP, Kim C-S, Saddler JN (2017) Alkali-oxygen impregnation prior to steam pretreating poplar wood chips enhances selective lignin modification and removal while maximizing carbohydrate recovery, cellulose accessibility, and enzymatic hydrolysis. ACS Sustain Chem Eng 5:4011–4017CrossRefGoogle Scholar
  14. 14.
    Chandra RP, Chu Q, Hu J, Zhong N, Lin M, Lee J-S, Saddler J (2016) The influence of lignin on steam pretreatment and mechanical pulping of poplar to achieve high sugar recovery and ease of enzymatic hydrolysis. Bioresour Technol 199:135–141CrossRefPubMedGoogle Scholar
  15. 15.
    Zhai R, Hu J, Saddler JN (2016) What are the major components in steam pretreated lignocellulosic biomass that inhibit the efficacy of cellulase enzyme mixtures? ACS Sustain Chem Eng 4:3429–3436CrossRefGoogle Scholar
  16. 16.
    Kumar S, Dheeran P, Singh SP, Mishra IM, Adhikari DK (2015) Bioprocessing of bagasse hydrolysate for ethanol and xylitol production using thermotolerant yeast. Bioproc Biosys Eng 38:39–47CrossRefGoogle Scholar
  17. 17.
    Menegol D, Scholl AL, Fontana RC, Dillon AJP, Camassola M (2014) Increased release of fermentable sugars from elephant grass by enzymatic hydrolysis in the presence of surfactants. Energy Convers Manage 88:1252–1256CrossRefGoogle Scholar
  18. 18.
    Qing Q, Yang B, Wyman CE (2010) Impact of surfactants on pretreatment of corn stover. Bioresour Technol 101:5941–5951CrossRefPubMedGoogle Scholar
  19. 19.
    Cao S, Aita GM (2013) Enzymatic hydrolysis and ethanol yields of combined surfactant and dilute ammonia treated sugarcane bagasse. Bioresour Technol 131:357–364CrossRefPubMedGoogle Scholar
  20. 20.
    Zhang H, Ye G, Wei Y, Li X, Zhang A, Xie J (2017) Enhanced enzymatic hydrolysis of sugarcane bagasse with ferric chloride pretreatment and surfactant. Bioresour Technol 229:96–103CrossRefPubMedGoogle Scholar
  21. 21.
    Goshadrou A, Lefsrud M (2017) Synergistic surfactant-assisted [EMIM]OAc pretreatment of lignocellulosic waste for enhanced cellulose accessibility to cellulase. Carbohydr Polym 166:104–113CrossRefPubMedGoogle Scholar
  22. 22.
    Nasirpour N, Mousavi SM, Shojaosadati SA (2014) A novel surfactant-assisted ionic liquid pretreatment of sugarcane bagasse for enhanced enzymatic hydrolysis. Bioresour Technol 169:33–37CrossRefPubMedGoogle Scholar
  23. 23.
    Mesquita JF, Ferraz A, Aguiar A (2016) Alkaline-sulfite pretreatment and use of surfactants during enzymatic hydrolysis to enhance ethanol production from sugarcane bagasse. Bioproc Biosyst Eng 39:441–448CrossRefGoogle Scholar
  24. 24.
    Okino S, Ikeo M, Ueno Y, Taneda D (2013) Effects of Tween 80 on cellulase stability under agitated conditions. Bioresour Technol 142:535–539CrossRefPubMedGoogle Scholar
  25. 25.
    Sindhu R, Kuttiraja M, Elizabeth Preeti V, Vani S, Sukumaran RK, Binod P (2013) A novel surfactant-assisted ultrasound pretreatment of sugarcane tops for improved enzymatic release of sugars. Bioresour Technol 135:67–72CrossRefPubMedGoogle Scholar
  26. 26.
    Börjesson J, Engqvist M, Sipos B, Tjerneld F (2007) Effect of poly (ethylene glycol) on enzymatic hydrolysis and adsorption of cellulase enzymes to pretreated lignocellulose. Enzyme Microb Technol 41:186–195CrossRefGoogle Scholar
  27. 27.
    Li Y, Sun Z, Ge X, Zhang J (2016) Effects of lignin and surfactant on adsorption and hydrolysis of cellulases on cellulose. Biotechnol Biofuels 9:20CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    Hsieh C-wC, Cannella D, Jørgensen H, Felby C, Thygesen LG (2015) Cellobiohydrolase and endoglucanase respond differently to surfactants during the hydrolysis of cellulose. Biotechnol Biofuels 8:52CrossRefPubMedPubMedCentralGoogle Scholar
  29. 29.
    Jin W, Chen L, Hu M, Sun D, Li A, Li Y, Hu Z, Zhou S, Tu Y, Xia T, Wang Y, Xie G, Li Y, Bai B, Peng L (2016) Tween-80 is effective for enhancing steam-exploded biomass enzymatic saccharification and ethanol production by specifically lessening cellulase absorption with lignin in common reed. Appl Energy 175:82–90CrossRefGoogle Scholar
  30. 30.
    Derbowka D, Andersen S, Lee-Andersen S, Stenberg C (2012) Poplar and willow cultivation and utilization Canada. 2008–2011 Canadian Country Progress Report. Conseil canadien des peupliers et des saules. Information, Publications, National reports prepared for IPC. http://www.poplar.ca/article/publications-131.asp (Page consultée le 15 mai, 2015
  31. 31.
    Sluiter JB, Ruiz RO, Scarlata CJ, Sluiter AD, Templeton DW (2010) Compositional analysis of lignocellulosic feedstocks. 1. Review and description of methods. J Agric Food Chem 58:9043–9053CrossRefPubMedPubMedCentralGoogle Scholar
  32. 32.
    Peltier C, Visalli M, Schlich P (2015) Comparison of canonical variate analysis and principal component analysis on 422 descriptive sensory studies. Food Qual Preference 40:326–333CrossRefGoogle Scholar
  33. 33.
    Mészáros E, Jakab E, Várhegyi G, Szepesváry P, Marosvölgyi B (2004) Comparative study of the thermal behavior of wood and bark of young shoots obtained from an energy plantation. J Anal Appl Pyrolysis 72:317–328CrossRefGoogle Scholar
  34. 34.
    Advanced Hardwood Biofuels Northwest (2013). Pre-treatment: hydrolysis releases sugars from wood, http://hardwoodbiofuels.org/conversion/pre-treatment
  35. 35.
    Agrawal R, Satlewal A, Kapoor M, Mondal S, Basu B (2017) Investigating the enzyme-lignin binding with surfactants for improved saccharification of pilot scale pretreated wheat straw. Bioresour Technol 224:411–418CrossRefPubMedGoogle Scholar
  36. 36.
    Eriksson T, Börjesson J, Tjerneld F (2002) Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzyme Microb Technol 31:353–364CrossRefGoogle Scholar
  37. 37.
    Rajasree KP, Mathew GM, Pandey A, Sukumaran RK (2013) Highly glucose tolerant β-glucosidase from Aspergillus unguis: NII 08123 for enhanced hydrolysis of biomass. J Ind Microbiol Biotechnol 40:967–975CrossRefPubMedGoogle Scholar
  38. 38.
    Verardi A, De Bari I, Ricca E, Calabro V (2011) Hydrolysis of ̀lignocellulosic biomass: current status of processes and technologies and future perspectives. In: Pinheiro Lima MA (ed) Bioethanol InTech, Rijeka, Croatia, 95–112Google Scholar
  39. 39.
    Yuqing Zhang XX, Zhang Y, Li J (2011) Effect of adding surfactant for transforming lignocellulose into fermentable sugars during biocatalysing. Biotechnol Bioproc Eng 16:930–936CrossRefGoogle Scholar
  40. 40.
    Benkun Qi XC, Fei Shen Y, Su, Wan Y (2009) Optimization of enzymatic hydrolysis of wheat straw pretreated by alkaline peroxide using response surface methodology. Ind Eng Chem Res 48:7346–7353CrossRefGoogle Scholar
  41. 41.
    Akihiko Sakurai MMaMS (2003) Effect of surfactants on phenol removal by the method of polymerization and precipitation catalysed by Coprinus cinereus peroxidase. J Chem Technol Biotechnol 78:952–958CrossRefGoogle Scholar
  42. 42.
    Rosgaard L, Andric P, Dam-Johansen K, Pedersen S, Meyer AS (2007) Effects of substrate loading on enzymatic hydrolysis and viscosity of pretreated barley straw. Appl Biochem Biotechnol 143:27–40CrossRefPubMedGoogle Scholar
  43. 43.
    Panagiotou G, Olsson L (2007) Effect of compounds released during pretreatment of wheat straw on microbial growth and enzymatic hydrolysis rates. Biotechnol Bioeng 96:250–258CrossRefPubMedGoogle Scholar
  44. 44.
    Jondiko TO, Yang L, Hays DB, Ibrahim AMH, Tilley M, Awika JM (2016) Prediction of wheat tortilla quality using multivariate modeling of kernel, flour, and dough properties. Innov Food Sci Emerg Technol 34:9–15CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • A. Alhammad
    • 1
  • P. Adewale
    • 1
  • M. Kuttiraja
    • 1
  • L. P. Christopher
    • 1
  1. 1.Biorefining Research InstituteLakehead UniversityThunder BayCanada

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