Enhancing enzyme-aided production of fermentable sugars from poplar pulp in the presence of non-ionic surfactants
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Addition of surfactants to enzymatic hydrolysis has been reported to enhance the hydrolytic potential of enzymes in the bioconversion of lignocellulosic biomass to fermentable sugars. The objective of this investigation was to evaluate the effects of four non-ionic surfactants (PEG4000, PEG8000, TitronX-100, and Tween80) on the efficiency of enzymatic hydrolysis of steam-pretreated poplar using a commercial cellulase preparation (Cellic® CTec2). Statistical discriminant analysis at four variable factors (surfactant type, surfactant concentration, hydrolysis time, and substrate consistency) revealed that enzymatic hydrolysis was significantly enhanced in the presence of PEG4000, with 19.2% increase in glucose yield over control without surfactant, whereas ANOVA test indicated substrate consistency and hydrolysis time as the most significant factors (P < 0.05). Hydrolysis of poplar pulp at 5% w/w pulp consistency with CTec2 in presence of 1% w/w PEG4000 produced the highest glucose yield of 58.5% after 96 h reaction time.
KeywordsEnzymatic hydrolysis Cellulase Poplar pulp Non-ionic surfactants Fermentable sugars Discriminant analysis
Financial support by the Biorefining Research Institute at Lakehead University is gratefully acknowledged.
- 13.Chu Q, Chandra RP, Kim C-S, Saddler JN (2017) Alkali-oxygen impregnation prior to steam pretreating poplar wood chips enhances selective lignin modification and removal while maximizing carbohydrate recovery, cellulose accessibility, and enzymatic hydrolysis. ACS Sustain Chem Eng 5:4011–4017CrossRefGoogle Scholar
- 29.Jin W, Chen L, Hu M, Sun D, Li A, Li Y, Hu Z, Zhou S, Tu Y, Xia T, Wang Y, Xie G, Li Y, Bai B, Peng L (2016) Tween-80 is effective for enhancing steam-exploded biomass enzymatic saccharification and ethanol production by specifically lessening cellulase absorption with lignin in common reed. Appl Energy 175:82–90CrossRefGoogle Scholar
- 30.Derbowka D, Andersen S, Lee-Andersen S, Stenberg C (2012) Poplar and willow cultivation and utilization Canada. 2008–2011 Canadian Country Progress Report. Conseil canadien des peupliers et des saules. Information, Publications, National reports prepared for IPC. http://www.poplar.ca/article/publications-131.asp (Page consultée le 15 mai, 2015
- 34.Advanced Hardwood Biofuels Northwest (2013). Pre-treatment: hydrolysis releases sugars from wood, http://hardwoodbiofuels.org/conversion/pre-treatment
- 38.Verardi A, De Bari I, Ricca E, Calabro V (2011) Hydrolysis of ̀lignocellulosic biomass: current status of processes and technologies and future perspectives. In: Pinheiro Lima MA (ed) Bioethanol InTech, Rijeka, Croatia, 95–112Google Scholar