An integrated bio-process for production of functional biomolecules utilizing raw and by-products from dairy and sugarcane industries
The study investigated an integrated bioprocessing of raw and by-products from sugarcane and dairy industries for production of non-digestible prebiotic and functional ingredients. The low-priced feedstock, whey, molasses, table sugar, jaggery, etc., were subjected to transglucosylation reactions catalyzed by dextransucrase from Leuconostoc mesenteroides MTCC 10508. HPLC analysis approximated production of about 11–14 g L−1 trisaccharide i.e. 2-α-d-glucopyranosyl-lactose (4-galactosyl-kojibiose) from the feedstock prepared from table sugar, jaggery, cane molasses and liquid whey, containing about 30 g L−1 sucrose and lactose each. The trisaccharide was hydrolysed into the prebiotic disaccharide, kojibiose, by employing recombinant β-galactosidase from Escherichia coli. The enzyme β-galactosidase achieved about 90% conversion of 2-α-d-glucopyranosyl-lactose into kojibiose. The d-fructose generated by catalytic reactions of dextransucrase was targeted for catalytic transformation into rare sugar, d-allulose (or d-psicose), by treating the samples with Smt3-d-psicose 3-epimerase. The catalytic reactions resulted in the conversion of ~ 25% d-fructose to d-allulose. These bioactive compounds are known to exert a plethora of benefits to human health, and therefore, are preferred ingredients for making functional foods.
Keywords2-α-d-Glucopyranosyl-lactose Kojibiose d-Allulose Dextran Cane molasses Liquid whey Table sugar Jaggery
The authors acknowledge the Department of Biotechnology (DBT), Government of India for facilitating the present work at Center of Innovative and Applied Bioprocessing (CIAB), Mohali, India. KL acknowledges Science and Engineering Research Board (SERB) for providing N-PDF fellowship (PDF/2016/000408). SPS acknowledges ECR/2016/001228 grant. The thematic disclosures herein are covered in Indian patent file no. 201711006155.
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