Improved fermentation performance to produce bioethanol from Gelidium amansii using Pichia stipitis adapted to galactose

  • Pailin Sukwong
  • Chae Hun Ra
  • In Yung Sunwoo
  • Sumate Tantratian
  • Gwi-Taek Jeong
  • Sung-Koo Kim
Research Paper
  • 32 Downloads

Abstract

This study employed a statistical method to obtain optimal hyper thermal acid hydrolysis conditions using Gelidium amansii (red seaweed) as a source of biomass. The optimal hyper thermal acid hydrolysis using G. amansii as biomass was determined as 12% (w/v) slurry content, 358.3 mM H2SO4, and temperature of 142.6 °C for 11 min. After hyper thermal acid hydrolysis, enzymatic saccharification was carried out. The total monosaccharide concentration was 45.1 g/L, 72.2% of the theoretical value of the total fermentable monosaccharides of 62.4 g/L based on 120 g dry weight/L in the G. amansii slurry. To increase ethanol production, 3.8 g/L 5-hydroxymethylfurfural (HMF) in the hydrolysate was removed by treatment with 3.5% (w/v) activated carbon for 2 min and fermented with Pichia stipitis adapted to high galactose concentrations via separate hydrolysis and fermentation. With complete HMF removal and the use of P. stipitis adapted to high galactose concentrations, 22 g/L ethanol was produced (yield 0.50). Fermentation with total HMF removal and yeast adapted to high galactose concentrations increased the fermentation performance and decreased the fermentation time from 96 to 36 h compared to traditional fermentation.

Keywords

Hyper thermal acid hydrolysis HMF Activated carbon Adaptation Bioethanol production 

Notes

Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2016R1D1A1A09918683), Korea.

References

  1. 1.
    Tenenbaum DJ (2008) Food vs. fuel: diversion of crops could cause more hunger. Environ Health Perspect 116:A254–A257CrossRefGoogle Scholar
  2. 2.
    Harun R, Yip JWS, Thiruvenkadam S, Ghani WAWAK., Cherrington T, Danquah MK (2014) Algal biomass conversion to bioethanol—a step-by-step assessment. Biotechnol J 9:73–86CrossRefGoogle Scholar
  3. 3.
    Yanagisawa M, Kawai S, Murata K (2013) Strategies for the production of high concentrations of bioethanol from seaweeds: production of high concentrations of bioethanol from seaweeds. Bioengineered 4:224–235CrossRefGoogle Scholar
  4. 4.
    Yoon JJ, Kim YJ, Kim SH, Ryu HJ, Choi JY, Kim GS, Shin MK (2010) Production of polysaccharides and corresponding sugars from red seaweed. Adv Mater Res 93–94:463–466CrossRefGoogle Scholar
  5. 5.
    Cho H, Ra CH, Kim S-K (2014) Ethanol production from the seaweed Gelidium amansii, using specific sugar acclimated yeasts. J Microbiol Biotechnol 24:264–269CrossRefGoogle Scholar
  6. 6.
    Lenihan P, Orozco A, O’Neill E, Ahmad MNM, Rooney DW, Walker GM (2010) Dilute acid hydrolysis of lignocellulosic biomass. Chem Eng J 156:395–403CrossRefGoogle Scholar
  7. 7.
    Meinita MDN, Hong Y-K, Jeong G-T (2012) Detoxification of acidic catalyzed hydrolysate of Kappaphycus alvarezii (cottonii). Bioprocess Biosyst Eng 35:93–98CrossRefGoogle Scholar
  8. 8.
    Saha BC, Iten LB, Cotta MA, Wu YV (2005) Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol. Process Biochem 40:3693–3700CrossRefGoogle Scholar
  9. 9.
    Ra CH, Nguyen TH, Jeong G-T, Kim S-K (2016) Evaluation of hyper thermal acid hydrolysis of Kappaphycus alvarezii for enhanced bioethanol production. Bioresour Technol 209:66–72CrossRefGoogle Scholar
  10. 10.
    Jeong G-T, Ra CH, Hong Y-K, Kim JK, Kong I-S, Kim S-K, Park D-H (2015) Conversion of red-algae Gracilaria verrucosa to sugars, levulinic acid and 5-hydroxymethylfurfural. Bioprocess Biosyst Eng 38:207–217CrossRefGoogle Scholar
  11. 11.
    Liu ZL, Ma M, Song M (2009) Evolutionarily engineered ethanologenic yeast detoxifies lignocellulosic biomass conversion inhibitors by reprogrammed pathways. Mol Genet Genom 282:233–244CrossRefGoogle Scholar
  12. 12.
    Escalante-Chong R, Savir Y, Carroll SM, Ingraham JB, Wang J, Marx CJ, Springer M (2015) Galactose metabolic genes in yeast respond to a ratio of galactose and glucose. Proc Natl Acad Sci 112:1636–1641CrossRefGoogle Scholar
  13. 13.
    Yoo CG, Lee CW, Kim TH (2011) Optimization of two-stage fractionation process for lignocellulosic biomass using response surface methodology (RSM). Biomass Bioenergy 35:4901–4909CrossRefGoogle Scholar
  14. 14.
    Kim HJ, Ra CH, Kim S-K (2013) Ethanol production from seaweed (Undaria pinnatifida) using yeast acclimated to specific sugars. Biotechnol Bioprocess Eng 18:533–537CrossRefGoogle Scholar
  15. 15.
    Kubicek CP (1982) beta-Glucosidase excretion by Trichoderma pseudokoningii: correlation with cell wall bound beta-1.3-glucanase activities. Arch Microbiol 132:349–354CrossRefGoogle Scholar
  16. 16.
    Mandels M, Andreotti R, Roche C (1976) Measurement of saccharifying cellulase. Biotechnol Bioeng Symp 21–33Google Scholar
  17. 17.
    Jol CN, Neiss TG, Penninkhof B, Rudolph B, De Ruiter GA (1999) A novel high-performance anion-exchange chromatographic method for the analysis of carrageenans and agars containing 3,6-anhydrogalactose. Anal Biochem 268:213–222CrossRefGoogle Scholar
  18. 18.
    Jeong TS, Kim YS, Oh KK (2011) Two-stage acid saccharification of fractionated Gelidium amansii minimizing the sugar decomposition. Bioresour Technol 102:10529–10534CrossRefGoogle Scholar
  19. 19.
    Jeong G-T, Park D-H (2010) Production of sugars and levulinic acid from marine biomass Gelidium amansii. Appl Biochem Biotechnol 161:41–52CrossRefGoogle Scholar
  20. 20.
    Rodrigues AC, Haven M, Lindedam J, Felby C, Gama M (2015) Celluclast and Cellic® CTec2: saccharification/fermentation of wheat straw, solid–liquid partition and potential of enzyme recycling by alkaline washing. Enzyme Microb Technol 79–80:70–77CrossRefGoogle Scholar
  21. 21.
    Kim N-J, Li H, Jung K, Chang HN, Lee PC (2011) Ethanol production from marine algal hydrolysates using Escherichia coli KO11. Bioresour Technol 102:7466–7469CrossRefGoogle Scholar
  22. 22.
    Zhao X, Moates GK, Elliston A, Wilson DR, Coleman MJ, Waldron KW (2015) Simultaneous saccharification and fermentation of steam exploded duckweed: improvement of the ethanol yield by increasing yeast titre. Bioresour Technol 194:263–269CrossRefGoogle Scholar
  23. 23.
    Guo Z, Olsson L (2014) Physiological response of Saccharomyces cerevisiae to weak acids present in lignocellulosic hydrolysate. FEMS Yeast Res 14:1234–1248CrossRefGoogle Scholar
  24. 24.
    Gütsch JS, Sixta H (2011) The HiTAC-process (high temperature adsorption on activated charcoal)—new possibilities in autohydrolysate treatment. Lenzing Ber 89:142–151Google Scholar
  25. 25.
    Ra CH, Jung JH, Sunwoo IY, Kang CH, Jeong GT, Kim S-K (2015) Detoxification of Eucheuma spinosum hydrolysates with activated carbon for ethanol production by the salt-tolerant yeast Candida tropicalis. J Microbiol Biotechnol 25:856–862CrossRefGoogle Scholar
  26. 26.
    Ra CH, Jeong G-T, Shin MK, Kim S-K (2013) Biotransformation of 5-hydroxymethylfurfural (HMF) by Scheffersomyces stipitis during ethanol fermentation of hydrolysate of the seaweed Gelidium amansii. Bioresour Technol 140:421–425CrossRefGoogle Scholar
  27. 27.
    Nguyen TH, Ra CH, Sunwoo IY, Jeong GT, Kim S-K (2016) Evaluation of galactose adapted yeasts for bioethanol fermentation from Kappaphycus alvarezii hydrolyzates. J Microbiol Biotechnol 26:1259–1266CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Pailin Sukwong
    • 1
  • Chae Hun Ra
    • 2
  • In Yung Sunwoo
    • 1
  • Sumate Tantratian
    • 3
  • Gwi-Taek Jeong
    • 1
  • Sung-Koo Kim
    • 1
  1. 1.Department of BiotechnologyPukyong National UniversityBusanKorea
  2. 2.Department of Food and BiotechnologyHankyong National UniversityGyeonggiKorea
  3. 3.Department of Food TechnologyChulalongkorn UniversityBangkokThailand

Personalised recommendations