Aspergillus flavus: fungal strain A5p1 is reported here to decolorize a variety of dyes under broad environmental conditions. For the 15 dyes tested, the decolorization efficiencies ranged from 61.7 to 100.0% at an initial concentration of 100 mg/L. Direct Blue 71 (DB71), Direct Blue 86 (DB86), and Reactive Blue 19 (RB19) were selected as models for comparing decolorization performance. The results show that biosorption and biodegradation work together in the strain to remove the pigments; the effect of biosorption was stronger for DB71 and DB86, whereas the effect of biodegradation was stronger for RB19. For DB71 and DB86, the decolorization rate surpassed 90% with inactivated biomass under acidic conditions and with living biomass under alkaline conditions. DB19 achieved the highest removal rate of 90% under neutral conditions. The strain could effectively decolorize high concentrations of dyes up to 1000 mg/L, which was achieved mainly via biosorption at concentrations below 500 mg/L and via biodegradation at concentrations above 500 mg/L. The findings suggest that A5p1 has a strong adaptability to different dye types and environmental conditions and can, therefore, be potentially used in biological processes for the treatment of dye-containing wastewater.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Perlatti B, Fernandes JB, Forim MR (2012) Validation and application of HPLC-ESI-MS/MS method for the quantification of RBBR decolorization, a model for highly toxic molecules, using several fungi strains. Biores Technol 124:37–44
Siddique M, Farooq R, Price GJ (2014) Synergistic effects of combining ultrasound with the Fenton process in the degradation of Reactive Blue 19. Ultrason Sonochem 21:1206–1212
Hayat K, Menhas S, Bundschuh J et al (2017) Microbial biotechnology as an emerging industrial wastewater treatment process for arsenic mitigation: a critical review. J Clean Prod 151:427–438
Ansari Z, Karimi A, Sedghi S et al (2017) Glucose oxidase effect on treatment of textile effluent containing reactive azo dyes by Phanerochaete chrysosporium. J Chem Technol Biotechnol 92:1721–1726
Junghanns C, Neumann JF, Schlosser D (2012) Application of the aquatic fungus Phoma sp. (DSM22425) in bioreactors for the treatment of textile dye model effluents. J Chem Technol Biotechnol 87:1276–1283
He F, Qin X, Zhang H et al (2015) Characterization of laccase isoenzymes from the white-rot fungus Ganoderma sp.En3 and synergistic action of isoenzymes for dye decolorization. J Chem Technol Biotechnol 90:2265–2279
Haq I, Kumar S, Kumari V et al (2016) Evaluation of bioremediation potentiality of ligninolytic Serratia liquefaciens for detoxification of pulp and paper mill effluent. J Hazard Mater 305:190–199
Mahmood F, Shahid M, Hussain S et al (2017) Potential plant growth-promoting strain Bacillus sp. SR-2–1/1 decolorized azo dyes through NADH-ubiquinone:oxidoreductase activity. Biores Technol 235:176–184
Ding J, Zhang Y, Xie Q et al (2015) Anaerobic biodecolorization of AO7 by a newly isolated Fe (III)-reducing bacterium Sphingomonas strain DJ. J Chem Technol Biotechnol 90:158–165
Meng X, Liu G, Zhou J et al (2014) Effects of redox mediators on azo dye decolorization by Shewanella algae, under saline conditions. Biores Technol 151:63–68
Baldev E, Mubarakali D, Ilavarasi A et al (2013) Degradation of synthetic dye, Rhodamine B to environmentally non-toxic products using microalgae. Colloids Surf B Biointerfaces 105:207–214
Akar T, Demir TA, Kiran I et al (2010) Biosorption potential of Neurospora crassa cells for decolorization of Acid Red 57 (AR57) dye. J Chem Technol Biotechnol 81:1100–1106
Li R, Ning XA, Sun J et al (2015) Decolorization and biodegradation of the Congo red by Acinetobacter baumannii YNWH 226, and its polymer production’s flocculation and dewatering potential. Biores Technol 194:233–239
Reddy A (1995) The potential for white-rot fungi in the treatment of pollutants. Curr Opin Biotechnol 6:320–328
Teerapatsakul C, Parra R, Keshavarz T et al (2017) Repeated batch for dye degradation in an airlift bioreactor by laccase entrapped in copper alginate. Int Biodeterior Biodegradation 120:52–57
Chen SH, Yien Ting AS (2015) Biodecolorization and biodegradation potential of recalcitrant triphenylmethane dyes by Coriolopsis sp. isolated from compost. J Environ Manage 150:274–280
Faraco V, Pezzella C, Giardina P et al (2009) Decolourization of textile dyes by the white-rot fungi Phanerochaete chrysosporium and Pleurotus ostreatus. J Chem Technol Biotechnol 84:414–419
Singh S, Pakshirajan K, Daverey A (2010) Enhanced decolourization of Direct Red-80 dye by the white rot fungus Phanerochaete chrysosporium employing sequential design of experiments. Biodegradation 21:501–511
Miranda RD, Gomes CMD, Pereira N, et al (2013) Biotreatment of textile effluent in static bioreactor by Curvularia lunata, URM 6179 and Phanerochaete chrysosporium, URM 6181. Biores Technol 142:361–367
Ghosh A, Dastidar MG, Sreekrishnan TR (2016) Bioremediation of a Chromium Complex Dye Using Aspergillus flavus and Aspergillus tamari. Chem Eng Technol 39:1636–1644
Andleeb S, Atiq N, Robson GD et al (2012) An investigation of anthraquinone dye biodegradation by immobilized Aspergillus flavus in fluidized bed bioreactor. Environ Sci Pollut Res 19:1728–1737
Hofrichter M, Vares K, Scheibner K et al (1999) Mineralization and solubilization of synthetic lignin by manganese peroxidases from Nematoloma frowardii, and Phlebia radiate. J Biotechnol 67:217–228
Bankar SB, Bule MV, Singhal RS et al (2009) Glucose oxidase—an overview. Biotechnol Adv 27:489–501
Fleischmann C, Lievenbrück M, Ritter H (2015) Polymers and dyes: developments and applications. Polymer 7:717–746
Esmaeili A, Kalantari M (2012) Bioremoval of an azo textile dye, Reactive Red 198, by Aspergillus flavus. World J Microbiol Biotechnol 28:1125–1131
Chen Y, Chen G, Chen L et al (2011) Review of Studies on Effects of Molecular Structure on Azo Dye Microbial Decolorization. Environ Sci Technol 34:65–69
Lin-Na D, Bing W, Gang L, Sheng W, David EC, Yu-Hua Z (2012) Biosorption of the metal-complex dye Acid Black 172 by live and heated biomass of Pseudomonas sp. Strain DYl: kinetics and sorption mechanisms. J Hazard Mater 205:47–54
Espinosa-Ortiz EJ, Rene ER, Pakshirajan K et al (2016) Fungal pelleted reactors in wastewater treatment: applications and perspectives. Chem Eng J 283:553–571
Wesenberg D, Kyriakides I, Agathos SN (2003) White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnol Adv 22:161–187
Lan J, Huang X, Hu M et al (2006) High efficient degradation of dyes with lignin peroxidase coupled with glucose oxidase. J Biotechnol 123:483–490
Binupriya AR, Sathishkumar M, Swaminathan K et al (2008) Comparative studies on removal of Congo red by native and modified mycelial pellets of Trametes versicolor in various reactor modes. Biores Technol 99:1080–1088
Namasivayam C, Kavitha D (2002) Removal of Congo Red from water by adsorption onto activated carbon prepared from coir pith, an agricultural solid waste. Dyes Pigment 54(1):47–58
Aksu Z, Tatlı Aİ, Tunç Ö (2008) A comparative adsorption/biosorption study of Acid Blue 161: effect of temperature on equilibrium and kinetic parameters. Chem Eng J 142:23–39
Permpornsakul P, Prasongsuk S, Lotrakul P et al (2016) Treatment of an azo dye reactive black 5 by tropical resupinate fungus Phanerochaete sordida, PBU 0057. New Biotechnol 33:135–136
Jin R, Hua Y, Zhang A et al (2009) Bioaugmentation on decolorization of C.I. Direct Blue 71 by using genetically engineered strain Escherichia coli, JM109 (pGEX-AZR. J Hazard Mater 163:1123–1128
Jasińska A, Paraszkiewicz K, Sip A et al (2015) Malachite green decolorization by the filamentous fungus Myrothecium roridum, mechanistic study and process optimization. Biores Technol 194:43–48
Paz A, Carballo J, Pérez MJ et al (2017) Biological treatment of model dyes and textile wastewaters. Chemosphere 181:168
Martins MAM, Lima N, Silvestre AJD et al (2003) Comparative studies of fungal degradation of single or mixed bioaccessible reactive azo dyes. Chemosphere 529:67–73
Pakshirajan K, Singh S (2010) Decolorization of synthetic wastewater containing azo dyes in a batch-operated rotating biological contactor reactor with the immobilized fungus Phanerochaete chrysosporium. Ind Eng Chem Res 49:7484–7487
This work was supported by the National Natural Science Foundation of China (Grants 21066001 and 51108098). The authors are grateful to American Journal Experts for English editing.
Conflict of interest
The authors declare that they have no conflict of interest.
Electronic supplementary material
Below is the link to the electronic supplementary material.
About this article
Cite this article
Ning, C., Qingyun, L., Aixing, T. et al. Decolorization of a variety of dyes by Aspergillus flavus A5p1. Bioprocess Biosyst Eng 41, 511–518 (2018). https://doi.org/10.1007/s00449-017-1885-9
- Aspergillus flavus
- Dye decolorization