Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Microbial community analysis of simultaneous ammonium removal and Fe3+ reduction at different influent ammonium concentrations

Abstract

This study investigates the impacts of influent ammonium concentrations on the microbial community in immobilized heterotrophic ammonium removal system. Klebsiella sp. FC61, the immobilized species, has the ability to perform simultaneous ammonium removal and Fe3+ reduction. It was found that average ammonium removal rate decreased from 0.308 to 0.157 mg/L/h, as the influent NH4 +-N was reduced from 20 to 10 mg/L. Meanwhile, at a total Fe3+ concentration of 20 mg/L, the average Fe3+ reduction removal efficiency and rate decreased from 44.61% and 0.18 mg/L/h, to 27.10% and 0.11 mg/L/h, respectively. High-throughput sequencing was used to observe microbial communities in bioreactor Samples B1, B2, and B3, after exposure to different influent NH4 +-N conditions. Results show that higher influent NH4 +-N concentrations increased microbial richness and diversity and that Klebsiella sp. FC61 play a functional role in the simultaneous removal of NH4 +-N and Fe3+ reduction in bioreactor systems.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. 1.

    Boon N, De Gelder L, Lievens H, Siciliano SD, Top EM, Verstraete W (2002) Bioaugmenting bioreactors for the continuous removal of 3-chloroaniline by a slow release approach. Environ Sci Technol 36:4698–4704

  2. 2.

    Chen D, Wang HY, Ji B, Yang K, Wei L, Jiang Y (2015) A high-throughput sequencing study of bacterial communities in anautohydrogenotrophic denitrifying bio-ceramsite reactor. Process Biochem 50:1904–1910

  3. 3.

    Cheng Z, Hu X, Sun ZR (2016) Microbial community distribution and dominant bacterial species analysis in the bio-electrochemical system treating low concentration cefuroxime. Chem Eng J 303:137–144

  4. 4.

    El-Latif Hesham A, Qi R, Yang M (2011) Comparison of bacterial community structures in two systems of a sewage treatment plant using PCR-DGGE analysis. J Environ Sci 23(12):2049–2054

  5. 5.

    Fykse EM, Aarskaug T, Madslien EH, Dybwad M (2016) Microbial community structure in a full-scale anaerobic treatment plant during start-up and first year of operation revealed by high-throughput 16s rrna gene amplicon sequencing. Bioresour Technol 222:380–387

  6. 6.

    Hao T, Wei L, Lu H, Chui HK, Mackey HR, Loosdrecht MCM, Chen GH (2013) Characterization of sulfate-reducing granular sludge in the SANI process. Water Res 47:7042–7052

  7. 7.

    Hao X, Wang Q, Zhang X, Cao Y, Mark Loosdrecht CMV (2009) Experimental evaluation of decrease in bacterial activity due to cell death and activity decayin activated sludge. Water Res 43:3604–3612

  8. 8.

    Huang G, Liu F, Yang Y, Deng W, Li S, Huang Y, Kong X (2015) Removal of ammonium-nitrogen from groundwater using a fully passive permeable reactive barrier with oxygen-releasing compound and clinoptilolite. J Environ Manag 154:1–7

  9. 9.

    Huang S, Jaffe PR (2015) Characterization of incubation experiments and development of an enrichment culture capable of ammonium oxidation under iron reducing conditions. Biogeosciences 12:769–779

  10. 10.

    Jin D, Wang P, Bai Z, Wang X, Peng H, Qi R, Yu Z, Zhuang G (2011) Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches. J Environ Sci China 23(11):1880–1887

  11. 11.

    Lee OO, Wang Y, Yang J, Lafi FF, Al-Suwailem A, Qian P (2010) Pyrosequencingreveals highly diverse and species-specific microbial communities in sponges from the Red Sea. ISME J 5:650–664

  12. 12.

    Lovley DR, Holmes DE, Kelly PN (2004) Dissimilatory Fe(III) and Mn(IV) reduction. Microb Physiol 49:119–186

  13. 13.

    Lv X, Jiang G, Xue X, Wu D, Sheng T, Sun C, Xu X (2013) Fe0-Fe3O4 nanocomposites embedded polyvinyl alcohol/sodium alginate beads for chromium (VI) removal. J Hazard Mater 262:748–758

  14. 14.

    Ma Z, Wen X, Zhao F, Xia Y, Huang X, Waite D, Guan J (2013) Effect of temperature variation on membrane fouling and microbial community structure in membrane bioreactor. Bioresour Technol 133:462–468

  15. 15.

    Mulla SI, Talwar MP, Bagewadi ZK, Hoskeri RS, Ninnekar HZ (2013) Enhanced degradation of 2-nitrotoluene by immobilized cells of Micrococcus sp. strain SMN-1. Chemosphere 90:1920–1924

  16. 16.

    Okoniewska E, Lach J, Kacprzak M, Neczaj E (2007) The removal of manganese, iron and ammonium nitrogen on impregnated activated carbon. Desalination 206:251–258

  17. 17.

    Scala DJ, Hacherl EL, Cowan R, Young LY, Kosson DS (2006) Characterization of Fe(III)-reducing enrichment cultures and isolation of Fe(III)-reducing bacteria from the Savannah River site, South Carol. Microbiology 157:772–783

  18. 18.

    Schloss PD, Gevers D, Westcott SL (2011) Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies. PLoS One 6(12):e0027310

  19. 19.

    Shelobolina ES, Nevin KP, Blakeney-hayward JD (2007) Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses. Syst Evol Microbiol 57:126–162

  20. 20.

    Shrestha J, Rich JJ, Ehrenfeld JG, Jaffe PR (2009) Oxidation of ammonium to nitrite under iron-reducing conditions in wetland soils: laboratory, field demonstrations, and push–pull rate determination. Soil Sci 174(3):156–164

  21. 21.

    Su JF, Cheng C, Huang TL, Ma F, Lu JS, Shao SC (2016) Novel simultaneous Fe(III) reduction and ammonium oxidation of sp. FC61 under the anaerobic conditions. Rsc Adv 6(15):12584–12591

  22. 22.

    Su JF, Cheng C, Ma F (2016) Comparison of the NH4 +-N removal ability by Klebsiella sp. FC61 in a bacterial suspension system and a bacterial immobilization system. Sep Purif Technol 66:106–114

  23. 23.

    Wang J, Li Q, Qi R, Tandoi V, Yang M (2014) Sludge bulking impact on relevant bacterial populations in a full-scale municipal wastewater treatment plant. Process Biochem 49(12):2258–2265

  24. 24.

    Weon SY, Lee CW, Lee SI, Koopman B (2002) Nitrite inhibition of aerobic growth of Acinetobacter sp. Water Res 36(18):4471–4476

  25. 25.

    Wilkinson L, Friendly M (2009) The history of the cluster heat map. Am Stat 63:179–184

  26. 26.

    Yang WH, Weber KA, Silver WL (2012) Nitrogen loss from soil through anaerobic ammonium oxidation coupled to iron reduction. Nat Geosci 5(8):538–541

  27. 27.

    Ye L, Zhang T (2013) Bacterial communities in different sections of a municipal wastewater treatment plant revealed by 16S rDNA 454 pyrosequencing. Appl Microbiol Biotechnol 97(6):2681–2690

  28. 28.

    Zhang T, Shao MF, Ye L (2012) 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants. ISME J 6(6):1137–1147

  29. 29.

    Zhang Y, Tian Z, Liu MM, Shi ZJ, Hale L, Zhou JZ, Yang M (2015) High concentrations of the antibiotic spiramycin in wastewater lead to high abundance of ammonia-oxidizing archaea in nitrifying populations. Environ Sci Technol 49:9124–9132

  30. 30.

    Zhou S, Huang T, Ngo HH, Zhang H, Fei L, Zeng M (2016) Nitrogen removal characteristics of indigenous aerobic denitrifiers and changes in the microbial community of a reservoir enclosure system via in situ oxygen enhancement using water lifting and aeration technology. Bioresour Technol 214:63–73

Download references

Acknowledgements

This research work was partly supported by the National Natural Science Foundation of China (NSFC) (No. 51678471), the National Key Research and Development Project (No. 2016YFC0200706), and the Science and technology overall Plan of Shaanxi Province under Grant (No. 2016KTCG01-17).

Author information

Correspondence to Jun feng Su or Li Wei.

Ethics declarations

Conflict of interest

The authors declared that they have no conflict of interest.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Su, J.f., Lian, T.t., Huang, T.l. et al. Microbial community analysis of simultaneous ammonium removal and Fe3+ reduction at different influent ammonium concentrations. Bioprocess Biosyst Eng 40, 1555–1563 (2017). https://doi.org/10.1007/s00449-017-1811-1

Download citation

Keywords

  • Ammonium removal
  • Fe3+ reduction
  • High-throughput sequencing
  • Microbial community