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Application of calcium phosphate flocculation in high-density cell culture fluid with high product titer of monoclonal antibody

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Abstract

The calcium phosphate [Ca3(PO4)2] precipitation was used for improving the clarification efficiency in harvest process of the monoclonal antibody (mAb) containing cell culture fluid (CCF) with high turbidity and product titer. The flocculation conditions (concentration, addition order of flocculants, pH, and operation time), and the effect of flocculants on the mAb physical chemical properties (such as distribution of charge variants and aggregates) and process-related impurities removal (such as DNA and CHOP) were evaluated in this study. The results showed that the turbidity of CCF supernatant was significantly reduced at pH 7, 120 min with addition of phosphate ions first, while a high mAb recovery yield was kept in the CCF supernatant after flocculation. Addition of calcium ions at 15–60 mM was sufficient for flocculation in this study. A relationship between turbidity/mAb recovery yield and the concentration of calcium ions was established. More than 85% DNA in the CCF were effectively removed by the addition of optimal concentration of flocculants. Flocculation process of Ca3(PO4)2 is an effective pretreatment method in purification processes of mAbs from the CCF with high turbidity and product titer.

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Acknowledgements

The authors would like to thank the colleagues from Mabworks Biotech Co., Ltd. for their help, such as Miao Zhu, Chunyang Jin, and Guangkai Zhu, for their help in flocculation operation experiments, and Chenying Xie, Chuan He, and Weihui Fu for their help in analytical assays.

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Correspondence to Hui Fang Liu.

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Chen, G., Su, Z., Li, F. et al. Application of calcium phosphate flocculation in high-density cell culture fluid with high product titer of monoclonal antibody. Bioprocess Biosyst Eng 40, 703–714 (2017). https://doi.org/10.1007/s00449-017-1735-9

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Keywords

  • Calcium phosphate
  • Flocculation
  • Monoclonal antibody
  • Pretreatment