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Bioprocess and Biosystems Engineering

, Volume 39, Issue 9, pp 1435–1440 | Cite as

A biotransformation process for the production of cucurbitacin B from its glycoside using a selected Streptomyces sp.

  • Jianfeng Mei
  • Sha Li
  • Hang Jin
  • Lan Tang
  • Yu Yi
  • Hong Wang
  • Guoqing YingEmail author
Original Paper

Abstract

Cucurbitacin B (CuB) and its glycoside, cucurbitacin B 2-o-β-d-glucoside (CuBg), abundantly occur in the pedicels of Cucumis melo. Compared with CuB, CuBg is not efficiently extracted from the pedicels. Furthermore, the anticancer activity of CuBg is lower than that of the aglycone. A process for CuBg biotransformation to CuB was developed for the first time. A strain of Streptomyces species that converts CuBg into CuB was isolated from an enrichment culture of C. melo pedicels. After optimization of conditions for enzyme production and biotransformation, a maximum conversion rate of 92.6 % was obtained at a CuBg concentration of 0.25 g/L. When biotransformation was performed on C. melo pedicel extracts, the CuB concentration in the extracts increased from 1.50 to 3.27 g/L. The conversion rate was almost 100 %. The developed process may be an effective biotransformation method for industrial production CuB from C. melo pedicels for pharmaceuticals.

Keywords

Cucurbitacin B 2-o-β-d-glucoside Cucurbitacin B Biotransformation Streptomyces sp. 

Notes

Acknowledgments

The authors gratefully acknowledge the financial support from the Natural Science Foundation of Zhejiang Province (Grant No. LY13B020011).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Jianfeng Mei
    • 1
  • Sha Li
    • 1
  • Hang Jin
    • 1
  • Lan Tang
    • 1
  • Yu Yi
    • 1
  • Hong Wang
    • 1
  • Guoqing Ying
    • 1
    Email author
  1. 1.College of Pharmaceutical ScienceZhejiang University of TechnologyHangzhouChina

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