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Effects of nitrogen on the lipid and carotenoid accumulation of oleaginous yeast Sporidiobolus pararoseus

Abstract

Nitrogen limited but carbon excess condition was used to obtain high cellular lipid content and production. The maximum lipid production was 51 g/L, the lipid content in the dry cell was 60 %, and the lipid productivity was 0.53 g/L/h. In the fermentation, the content of lipid was raised from 20 % of dry cell weight to 60 %, and the proportion of oleic acid was raised from 66.8 to 72.5 %. Meanwhile, the metabolism of carotenoids switched to torulene, and its proportion was raised from 30 to 58 %. This was according to torulene had the better antioxidant ability than β-carotene to protect the strain from oxidative damage proved by their ABTS* radical scavenging activity and lipid peroxidation inhibition ability. Sporidiobolus pararoseus lipid was a good source of lipid not only because of its high oleic acid composition, but also the antioxidant ability of carotenoids in the lipid.

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Acknowledgments

A project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Author information

Correspondence to Wei-Guo Zhang.

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Han, M., Xu, Z., Du, C. et al. Effects of nitrogen on the lipid and carotenoid accumulation of oleaginous yeast Sporidiobolus pararoseus . Bioprocess Biosyst Eng 39, 1425–1433 (2016). https://doi.org/10.1007/s00449-016-1620-y

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Keywords

  • Sporidiobolus pararoseus
  • Oleaginous yeast
  • Carotenoid
  • Biodiesel