Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Phyto-synthesis of silver nanoparticles using Alternanthera tenella leaf extract: an effective inhibitor for the migration of human breast adenocarcinoma (MCF-7) cells

Abstract

In this study, phyto-synthesis of silver nanoparticles (AgNPs) was achieved using an aqueous leaf extract of Alternanthera tenella. The phytochemical screening results revealed that flavonoids are responsible for the AgNPs formation. The AgNPs were characterised using UV–visible spectrophotometer, field emission scanning microscopy/energy dispersive X-ray, transmission electron microscopy, fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction. The average size of the nanoparticles was found to be ≈48 nm. The EDX results show that strong signals were observed for the silver atoms. The strong band appearing at 1601–1595 cm−1 correspond to C–C stretching vibration from dienes in FT-IR spectrum indicating the formation of AgNPs. Human breast adenocarcinoma (MCF-7) cells treated with various concentrations of AgNPs showed a dose-dependent increase in cell inhibition. The IC50 value of the AgNPs was calculated to be 42.5 μg mL−1. The AgNPs showed a significant reduction in the migration of MCF-7 cells.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. 1.

    Schröfel A, Kratošová G, Šafarík I, Šafaríková M, Raška I, Shor LM (2014) Applications of biosynthesized metallic nanoparticles—a review. Acta Biomater 10:4023–4042

  2. 2.

    Li H, Xu D (2014) Silver nanoparticles as labels for applications in bioassays. Trends Anal Chem 61:67–73

  3. 3.

    Kathiravan V, Ravi S, Ashokkumar S (2014) Synthesis of silver nanoparticles from Melia dubia leaf extract and their in vitro anticancer activity. Spectrochim Acta A 130:116–121

  4. 4.

    Reddy NJ, Vali DN, Rani M, Rani SS (2014) Evaluation of antioxidant, antibacterial and cytotoxic effects of green synthesized silver nanoparticles by Piper longum fruit. Mater Sci Eng, C 34:115–122

  5. 5.

    Cheng F, Betts JW, Kelly SM, Schaller J, Heinze T (2013) Synthesis and antibacterial effects of aqueous colloidal solutions of silver nanoparticles using aminocellulose as a combined reducing and capping reagent. Green Chem 15:989–998

  6. 6.

    Kohler JM, Abahmane L, Wagner J, Albert J, Mayer G (2008) Preparation of metal nanoparticles with varied composition for catalytical applications in microreactors. Chem Eng Sci 63:5048–5055

  7. 7.

    Yu DG (2007) Formation of colloidal silver nanoparticles stabilized by Na+-poly(γ-glutamic acid)–silver nitrate complex via chemical reduction process. Colloids Surf B 59:171–178

  8. 8.

    Smetana AB, Klabunde KJ, Sorensea CM (2005) Synthesis of spherical silver nanoparticles by digestive ripening, stabilization with various agents, and their 3-D and 2-D superlattice formation. J Colloid Interf Sci 284:521–526

  9. 9.

    Senapati S, Ahmad A, Khan MI, Sastry M, Kumar R (2005) Extracellular biosynthesis of bimetallic Au-Ag alloy nanoparticles. Small 1:517–520

  10. 10.

    Shahverdi AR, Minaeian S, Shahverdi HR, Jamalifar H, Nohi AA (2007) Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria: a novel biological approach. Process Biochem 42:919–923

  11. 11.

    Inbathamizh L, Ponnu TM, Mary EJ (2013) In vitro evaluation of antioxidant and anticancer potential of Morinda pubescens synthesized silver nanoparticles. J Pharm Res 6:32–38

  12. 12.

    Anand KKH, Mandal BK (2015) Activity study of biogenic spherical silver nanoparticles towards microbes and oxidants. Spectrochim Acta A 135:639–645

  13. 13.

    Hosamani KM, Ganjihal SS, Chavadi DV (2004) Alternanthera triandra seed oil: a moderate source of ricinoleic acid and its possible industrial utilization. Ind Crop Prod 19:133–136

  14. 14.

    Biella CA, Salvador MJ, Dias DA, Dias-Baruffi M, Pereira-Crott LS (2008) Evaluation of immunomodulatory and anti-inflammatory effects and phytochemical screening of Alternanthera tenella Colla (Amaranthaceae) aqueous extracts. Mem Inst Oswaldo Cruz 103:569–577

  15. 15.

    Tripoli E, Guardia ML, Majo S, Giammanco DD, Giammanco M (2007) Citrus flavonoids: molecular structure, biological activity and nutritional properties: a review. Food Chem 104:466–479

  16. 16.

    Stephen A, Seethalakshmi S (2013) Phytochemical synthesis and preliminary characterization of silver nanoparticles using Hesperidin. J Nanosci 126564:1–6

  17. 17.

    de Rijke E, Out P, Niessen WMA, Ariese F, Gooijer C, Brinkman UAT (2006) Analytical separation and detection methods for flavonoids. J Chromatogr A 1112:31–63

  18. 18.

    Chebil L, Humeau C, Falcimaigne A, Engasser JM, Ghoul M (2006) Enzymatic acylation of flavonoids. Process Biochem 41:2237–2251

  19. 19.

    Pal RS, Girhepunje G, Ariharasivakumar K, Upadhyay A (2009) In vitro antioxidative activity of phenolic and flavonoid compounds extracted from seeds of Abrus precatorius. Int J Pharm Pharm Sci 1:136–140

  20. 20.

    Bitis L, Kultur S, Melkoglu G, Ozsoy N, Can A (2010) Flavonoids and antioxidant activity of Rosa agrestis leaves. Nat Prod Res 24:580–589

  21. 21.

    Salvador MJ, Ferreira EO, Mertens-Talcott SU, Castro WV, Butterweck V, Derendorf H, Dias DA (2006) HPLC quantitative analysis of antioxidant flavonoids from Alternanthera tenella Colla. Z Naturforsch 61:19–25

  22. 22.

    World Cancer Report (2014) World Health Organization 92-832-0429-8

  23. 23.

    Zohra SF, Merium B, Samira S, Muneer A (2012) Phytochemical screening and identification of some compounds from mallow. J Nat Prod Plant Resour 2:512–516

  24. 24.

    Chang TT, More SV, Lu IH, Hsu JC, Chen TJ, Jen YC, Lu CK, Li WS (2011) Isomalyngamide A, A-1 and their analogs suppress cancer cell migration in vitro. Eur J Med Chem 46:3810–3819

  25. 25.

    Vidhu VK, Aromal SS, Philip D (2011) Green synthesis of silver nanoparticles using Macrotyloma uniflorum. Spectrochim Acta A 83:392–397

  26. 26.

    Li Y, Ding Y (2012) Minireview: therapeutic potential of myricetin in diabetes mellitus. Food Sci Human Wellness 1:19–25

  27. 27.

    Dubey SP, Lahtinen M, Sillanpää M (2010) Green synthesis and characterizations of silver and gold nanoparticles using leaf extract of Rosa rugosa. Colloid Surf A 364:34–41

  28. 28.

    Bankar A, Joshi B, Kumar AR, Zinjarde S (2010) Banana peel extract mediated novel route for the synthesis of silver nanoparticles. Colloids Surf A 368:8–63

  29. 29.

    Mariselvam R, Ranjitsingh AJA, Nanthini AUR, Kalirajan K, Padmalatha C, Selvakumar PM (2014) Green synthesis of silver nanoparticles from the extract of the inflorescence of Cocos nucifera (Family: Arecaceae) for enhanced antibacterial activity. Spectrochim Acta A 129:537–541

  30. 30.

    Shankar SS, Rai A, Ahamad A, Sastry M (2005) Controlling the optical properties of Lemongrass extract synthesized gold nanotriangles and potential application in infrared-absorbing optical coatings. Chem Mater 17:566

  31. 31.

    Ramteke C, Chakrabarti T, Sarangi BK, Pandey RA (2013) Synthesis of silver nanoparticles from the aqueous extract of leaves of Ocimum sanctum for enhanced antibacterial activity. J Chem 278925:7

  32. 32.

    Arokiyaraj S, Arasu MV, Vincent S, Prakash NU, Choi SH, Oh YK, Choi KC, Kim KH (2014) Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study. Int J Nanomed 9:379–388

  33. 33.

    Muniyappan N, Nagarajan NS (2014) Green synthesis of silver nanoparticles with Dalbergia spinosa leaves and their applications in biological and catalytic activities. Process Biochem 49:1054–1061

  34. 34.

    Mittal AK, Kumar J, Mittal S, Banerjee UC (2014) Quercetin and gallic acid mediated synthesis of bimetallic (silver and selenium) nanoparticles and their antitumor and antimicrobial potential. J Colloid Interf Sci 431:194–199

  35. 35.

    Kumar DA, Palanichamy V, Roopan SM (2014) Green synthesis of silver nanoparticles using Alternanthera dentata leaf extract at room temperature and their antimicrobial activity. Spectrochim Acta A 127:168–171

  36. 36.

    Vasanth K, Ilango K, MohanKumar R, Agrawal A, Dubey GP (2014) Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction. Colloids Surf B 117:354–359

  37. 37.

    Asharani PV, Hande MP, Valiyaveettil S (2009) Anti-proliferative activity of silver nanoparticles. BMC Cell Biol 10:65

  38. 38.

    Nakkala JR, Mata R, Gupta AK, Sadras SR (2014) Biological activities of green silver nanoparticles synthesized with Acorous calamus rhizome extract. Eur J Med Chem 85:784–794

  39. 39.

    Kanipandian N, Thirumurugan R (2014) A feasible approach to phyto-mediated synthesis of silver nanoparticles using industrial crop Gossypium hirsutum (cotton) extract as stabilizing agent and assessment of its in vitro biomedical potential. Ind Crop Prod 55:1–10

  40. 40.

    Sankar R, Karthik A, Prabu A, Karthik S, Shivashangari KS, Ravikumar V (2013) Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity. Colloids Surf B 108:80–84

  41. 41.

    Rajkuberan C, Sudha K, Sathishkumar G, Sivaramakrishnan S (2015) Antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of Calotropis gigantea L. Spectrochim Acta A 136:924–930

  42. 42.

    Ramar M, Manikandan B, Raman T, Arunagirinathan K, Prabhu NM, Basu MJ, Perumal M, Palanisamy S, Munusamy A (2015) Biosynthesis of silver nanoparticles using ethanolic petals extract of Rosa indica and characterization of its antibacterial, anticancer and anti-inflammatory activities. Spectrochim Acta A 138:120–129

  43. 43.

    Weng CJ, Yen GC (2012) Chemopreventive effects of dietary phytochemicals against cancer invasion and metastasis: phenolic acids, monophenol, polyphenols, and their derivatives. Cancer Treat Rev 38:76–87

  44. 44.

    Jayakumar R, Kanthimathi MS (2012) Dietary spices protect against hydrogen peroxide-induced DNA damage and inhibit nicotine-induced cancer cell migration. Food Chem 134:1580–1584

  45. 45.

    Sun F, Zheng XY, Ye J, Wu TT, Wang J, Chen W (2012) Potential anticancer activity of myricetin in human T24 bladder cancer cells both in vitro and in vivo. Nutr Cancer 64:599–606

Download references

Acknowledgments

The first author P. Sathishkumar is grateful to the Universiti Teknologi Malaysia for providing a postdoctoral grant to carry out this research successfully. A part of this research was financially supported by Fundamental Research Grant Scheme from Ministry of Education, Malaysia (Vote R.J130000.7809.4F465) which is gratefully acknowledged.

Author information

Correspondence to Abdull Rahim Mohd Yusoff or Thayumanavan Palvannan.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Sathishkumar, P., Vennila, K., Jayakumar, R. et al. Phyto-synthesis of silver nanoparticles using Alternanthera tenella leaf extract: an effective inhibitor for the migration of human breast adenocarcinoma (MCF-7) cells. Bioprocess Biosyst Eng 39, 651–659 (2016). https://doi.org/10.1007/s00449-016-1546-4

Download citation

Keywords

  • Alternanthera tenella
  • Anticancer activity
  • Cell migration assay
  • Flavonoids
  • Human breast adenocarcinoma cells
  • Silver nanoparticles