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Saccharification of orange peel wastes with crude enzymes from new isolated Aspergillus japonicus PJ01

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Abstract

This study investigated the saccharification of orange peel wastes with crude enzymes from Aspergillus japonicus PJ01. Pretreated orange peel powder was hydrolyzed by submerged fermentation (SmF) and solid-state fermentation (SSF) crude enzymes, the results showed that 4 % (w/v) of solid loading, undiluted crude enzymes, and 45 °C were suitable saccharification conditions. The hydrolysis kinetics showed that the apparent Michaelis–Menten constant \(K_{{\text{m}_{app} }}\) and maximal reaction rate \(V_{{\max_{app} }}\) were 73.32 g/L and 0.118 g/(L min) for SmF enzyme, and 41.45 g/L and 0.116 g/(L min) for SSF enzyme, respectively. After 48 h of hydrolysis, the saccharification yields were 58.5 and 78.7 %, the reducing sugar concentrations were 14.9 and 20.1 mg/mL by SmF and SSF enzymes. Material balance showed that the SmF enzymatic hydrolysate was enriched galacturonic acid > arabinose > galactose > xylose, and the SSF enzymatic hydrolysate was enriched galacturonic acid > xylose > galactose > arabinose.

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Acknowledgments

We thank Dr. Yi Yang (Central South University) for valuable help with the language polishing. This work was supported by a Grant from the National High Technology Research and Development Program of China (863 Program) (No. 2011AA100804), Hunan Provincial Innovation Foundation for Postgraduate (CX2013B084), and Open-End Fund for the Valuable and Precision Instruments of Central South University (CSUZC2014003).

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Correspondence to Jin-lan Xia or Yang Shan.

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Li, P., Xia, J., Nie, Z. et al. Saccharification of orange peel wastes with crude enzymes from new isolated Aspergillus japonicus PJ01. Bioprocess Biosyst Eng 39, 485–492 (2016). https://doi.org/10.1007/s00449-015-1531-3

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Keywords

  • Saccharification
  • Orange peel
  • Enzyme
  • Kinetic parameter