Cost reduction on cellulase enzyme usage has been the central effort in the commercialization of fuel ethanol production from lignocellulose biomass. Therefore, establishing an accurate evaluation method on cellulase enzyme cost is crucially important to support the health development of the future biorefinery industry. Currently, the cellulase cost evaluation methods were complicated and various controversial or even conflict results were presented. To give a reliable evaluation on this important topic, a rigorous analysis based on the Aspen Plus flowsheet simulation in the commercial scale ethanol plant was proposed in this study. The minimum ethanol selling price (MESP) was used as the indicator to show the impacts of varying enzyme supply modes, enzyme prices, process parameters, as well as enzyme loading on the enzyme cost. The results reveal that the enzyme cost drives the cellulosic ethanol price below the minimum profit point when the enzyme is purchased from the current industrial enzyme market. An innovative production of cellulase enzyme such as on-site enzyme production should be explored and tested in the industrial scale to yield an economically sound enzyme supply for the future cellulosic ethanol production.
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Klein-Marcuschamer D, Oleskowicz-Popiel P, Simmons BA, Blanch HW (2012) The challenge of enzyme cost in the production of lignocellulosic biofuels. Biotechnol Bioeng 109:1083–1087
Chovau S, Degrauwe D, Bruggen BV (2013) Critical analysis of techno-economic estimates for the production cost of lignocellulosic bio-ethanol. Renew Sust Energ Rev 26:307–321
Geddes CC, Nieves IU, Ingram LO (2011) Advances in ethanol production. Curr Opin Biotechnol 22:312–319
Sassner P, Galbe M, Zacchi G (2008) Techno-economic evaluation of bioethanol production from three different lignocellulosic materials. Biomass Bioenerg 32:422–430
Aden A, Ruth M, Ibsen K, Jechura J, Neeves K, Sheehan J, Wallace B (2002) Lignocellulosic biomass to ethanol process design and economics utilizing co-current dilute acid prehydrolysis and enzymatic hydrolysis for corn stover. National Renewable Energy Laboratory, Golden
Wingren A, Galbe M, Roslander C, Rudolf A, Zacchi G (2005) Effect of reduction in yeast and enzyme concentrations in a simultaneous-saccharification-and-fermentation-based bioethanol process: technical and economic evaluation. Appl Biochem Biotech 122:485–499
Kazi FK, Fortman J, Anex R, Kothandaraman G, Hsu D, Aden A, Dutta A (2010) Techno-economic analysis of biochemical scenarios for production of cellulosic ethanol. National Renewable Energy Laboratory, Golden
Phitsuwan P, Laohakunjit N, Kerdchoechuen O, Kyu KL, Ratanakanokchai K (2013) Present and potential applications of cellulases in agriculture, biotechnology, and bioenergy. Folia Microbiol 58:163–176
Merino ST, Cherry J (2007) Progress and challenges in enzyme development for biomass utilization. Adv Biochem Engin Biotechnol 108:95–120
Hong Y, Nizami AS, Pourbafrani M, Saville BA, MacLean HL (2013) Impact of cellulase production on environmental and financial metrics for lignocellulosic ethanol. Biofuels Bioprod Bioref 7:303–313
Takimura O, Yanagida T, Fujimoto S, Minowa T (2013) Estimation of bioethanol production cost from rice straw by on-site enzyme production. J JPA Petrol Inst 56:150–155
Humbird D, David R, Tao L, Kinchin C, Hsu D, Aden A (2011) Process design and economics for biochemical conversion of lignocellulosic biomass to ethanol: dilute-acid pretreatment and enzymatic hydrolysis of corn stover. National Renewable Energy Laboratory, Golden
Wooley R, Putsche V (1996) Development of an ASPEN PLUS physical property database for biofuels components. National Renewable Energy Laboratory, Golden
Fang ZH, Zhang J, Lu QM, Bao J (2013) Process development of short-chain polyols synthesis from corn stover by combination of enzymatic hydrolysis and catalytic hydrogenolysis. Biotechnol Rep 3:15–20
Kovacs K, Szakacs G, Zacchi G (2009) Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride. Bioresour Technol 100:1350–1357
Deanda K, Zhang M, Eddy C, Picataggio S (1996) Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering. Appl Environ Microb 62:4465–4470
Rudolf A, Baudel H, Zacchi G, Hahn-Hagerdal B (2007) Simultaneous saccharification and fermentation of steam-pretreated bagasse using Saccharomyces cerevisiae TMB3400 and Pichia stipitis CBS6054. Biotechnol Bioeng 99:783–790
Wyman CE, Spindler DD, Grohmann K (1992) Simultaneous saccharification and fermentation of several lignocellulosic feedstocks to fuel ethanol. Biomass Bioenerg 3:301–307
Olofsson K, Bertilsson M, Liden G (2008) A short review on SSF—an interesting process option for ethanol production from lignocellulosic feedstocks. Biotechnol Biofuels 1:1–14
Kumar L, Arantes V, Chandra R, Saddler J (2012) The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility. Bioresour Technol 103:201–208
Schubert C (2006) Can biofuels finally take center stage? Nat Biotechnol 24:777–784
This research was supported by the National High-Tech Program of China (2012AA022301, 2014AA021901) and the Natural Science Foundation of China (21306048).
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Liu, G., Zhang, J. & Bao, J. Cost evaluation of cellulase enzyme for industrial-scale cellulosic ethanol production based on rigorous Aspen Plus modeling. Bioprocess Biosyst Eng 39, 133–140 (2016). https://doi.org/10.1007/s00449-015-1497-1
- Cost evaluation
- Aspen Plus modeling