Bioprocess and Biosystems Engineering

, Volume 38, Issue 11, pp 2129–2136 | Cite as

The roles of different salts and a novel osmotic pressure control strategy for improvement of DHA production by Schizochytrium sp.

  • Xue-Chao Hu
  • Lu-Jing Ren
  • Sheng-Lan Chen
  • Li Zhang
  • Xiao-Jun Ji
  • He HuangEmail author
Original Paper


The effects of different osmotic pressure, changed by six salts (NaCl, Na2SO4, (NH4)2SO4, KH2PO4 and MSG), on cell growth and DHA synthesis by Schizochytrium sp. were investigated. Six optimal mediums were obtained to study different osmotic pressure combinations at cell growth stage and DHA synthesis stage. Results showed that cultivated cell in higher osmotic pressure condition and fermented in lower osmotic pressure condition was benefit to enhance DHA synthesis. Combination 17-6 could get the maximum cell dry weight of 56.95 g/L and the highest DHA percentage in total fatty acids of 55.21 %, while combination 17-B could get the highest lipid yield of 33.47 g/L with 42.10 % DHA in total fatty acids. This was the first report about the enhancement of DHA production by osmotic regulation and this work provided two novel osmotic control processes for high lipid yield and high DHA percentage in total fatty acids.


Schizochytrium sp. Osmotic pressure Docosahexaenoic acid Salts 



This work was financially supported by the National Science Foundation for Distinguished Young Scholars of China (No. 21225626), the National Natural Science Foundation of China (no. 21306085), the National Science and Technology Pillar Program (no. 2011BAD23B03), the National High Technology Research and Development Program of China (No. 2012AA021704 and No. 2014AA021701) and the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20133221120008).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Xue-Chao Hu
    • 1
  • Lu-Jing Ren
    • 1
  • Sheng-Lan Chen
    • 1
  • Li Zhang
    • 1
  • Xiao-Jun Ji
    • 1
  • He Huang
    • 1
    Email author
  1. 1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjingPeople’s Republic of China

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