This study aimed to enhance Aspergillus ficuum phytase production in fed-batch and continuous fermentations with addition of talcum microparticles. Phytase activity almost doubled in fed-batch and continuous fermentations by addition of 15 g/l of talcum compared to the control. Effect of talcum on fungal morphology was also shown that addition of talcum provided smaller fungal pellets and more homogenized fermentation broth compared to the control. Average fungal pellet radius decreased from 500 to 100 µm by addition of 15 g/l of talcum in the bioreactors. Also, 15 g/l talcum addition increased phytase productivity and optimum dilution rate in the continuous fermentations from 0.293 to 0.621 U/ml/h and from 0.09 to 0.1/h, respectively, compared to control.
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Vohra A, Satyanarayana T (2003) Phytases: microbial sources, production, purification, and potential biotechnological applications. Cr Rev Biotechnol 23:29–60
Haefner SR, Knietsch A, Scholten E, Braun J, Lohscheidt M, Zelde O (2005) Biotechnological production and applications of phytases. Appl Microbiol Biotechnol 68:588–597
Sanson A, Etzion Z, Shanyp S, Berlyne GM, Yawl R (1981) Growth and bone mineralization as affected by dietary calcium, phytic acid and vitamin D. Comp Biochem Physiol 72:43–48
Mullaney EJ, Daly CB, Ulah AHJ (2000) Advances in phytase research. Adv Appl Microbiol 47:157–199
Sebastian S, Touchburn SP, Chavez ER, Lague PC (1996) The effects of supplemental microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper, and zinc in broiler chickens fed corn-soybean diets. Poult Sci 75:729–736
Nelson TS, Shieh TR, Wodzinski RJ, Ware JH (1968) The availability of phytate phosphorus in soybean meal before and after treatment with a mold phytase. Poult Sci 47:1842–1848
Cowieson AJ, O’Neil HM, Bedford MR (2012) Enzymes beyond phytase in poultry nutrition. Faculty of Veterinary Science, University of Sydney, Poultry Research Foundation, Sydney, Australia
Shah P, Bhavsar K, Soni SK, Khire JM (2009) Strain improvement and up scaling of phytase production by Aspergillus niger NCIM 563 under submerged fermentation conditions. J Ind Microbiol Biotechnol 36:373–380
Bhavsar K, Gujar P, Shah P, Kumar VR, Khire JM (2013) Combinatorial approach of statistical optimization and mutagenesis for improved production of acidic phytase by Aspergillus niger NCIM 563 under submerged fermentation condition. Appl Microbiol Biotechnol 97:673–679
Coban HB, Demirci A (2015) Improved submerged Aspergillus ficuum phytase production in bench-top bioreactors by optimization of fermentation medium. Acta Aliment (in press)
Coban HB, Demirci A (2014) Screening of phytase producers and optimization of culture conditions for submerged fermentation. Bioprocess Biosyst Eng 37:609–616
Jin H, Zheng Z, Gao M, Duan Z, Shi Z, Wang Z, Jin J (2007) Effective induction of phytase in Pichia pastoris fed-batch culture using an ANN pattern recognition model-based on-line adaptive control strategy. Biochem Eng J 37:26–33
Kleist S, Miksch G, Hitzmann B, Arndt M, Friehs K, Flaschel E (2003) Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies. Appl Microbiol Biotechnol 61:456–462
Hidayat BJ, Eriksen NT, Wiebe MG (2006) Acid phosphatase production by Aspergillus niger N402A in continuous flow culture. FEMS Microbiol Lett 254:324–331
Kaup BA, Ehrich K, Pescheck M, Schrader J (2008) Microparticle-enhanced cultivation of filamentous microorganisms: increased chloroperoxidase formation by Caldariomyces fumago as an example. Biotechnol Bioeng 99:491–498
Walisko R, Wittmann C, Krull R, Schrader J (2012) Microparticle based morphology engineering of filamentous microorganisms for industrial bio-production. Biotechnol Lett 34:1975–1982
Driouch H, Wittmann C, Hansch R, Wucherpfennig T, Krull R (2012) Improved enzyme production by bio-pellets Aspergillus niger: targeted morphology engineering using titanate microparticles. Biotechnol Bioeng 109:462–471
Driouch H, Roth A, Dersch P, Wittmann C (2011) Filamentous fungi in good shape microparticles for tailor-made fungal morphology and enhanced enzyme production. Bioeng Bugs 2:100–104
Coban HB, Demirci A, Turhan I (2015) Microparticle enhanced Aspergillus ficuum phytase production in submerged fermentations. Bioprocess Biosyst Eng. doi:10.1007/s00449-014-1349-4
Coban HB, Demirci A (2014) Enhanced submerged Aspergillus ficuum phytase production by implementation of fed-batch fermentation. Bioprocess Biosyst Eng 37:2579–2586
Mittal A, Singh G, Goyal V, Yadav A, Aggarwal NK (2012) Production of phytase by acido-thermophilic strain of Klebsiella sp. DB-3FJ711774.1 using orange peel flour under submerged fermentation. Innov Rom Food Biotechnol 10:18–27
This work was supported in part by Turkish Ministry of Education by providing scholarship to Hasan Bugra Coban and the Pennsylvania Agricultural Experiment Station.
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Coban, H.B., Demirci, A. & Turhan, I. Enhanced Aspergillus ficuum phytase production in fed-batch and continuous fermentations in the presence of talcum microparticles. Bioprocess Biosyst Eng 38, 1431–1436 (2015). https://doi.org/10.1007/s00449-015-1384-9
- Aspergillus ficuum
- Fed-batch fermentation
- Continuous fermentation