Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Biosynthesis of fluorescent gold nanoparticles using an edible freshwater red alga, Lemanea fluviatilis (L.) C.Ag. and antioxidant activity of biomatrix loaded nanoparticles

  • 516 Accesses

  • 20 Citations

Abstract

Biosynthesis of gold nanoparticles has been accomplished via reduction of an aqueous chloroauric acid solution with the dried biomass of an edible freshwater epilithic red alga, Lemanea fluviatilis (L.) C.Ag., as both reductant and stabilizer. The synthesized nanoparticles were characterized by UV–visible, powder X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), and dynamic light scattering (DLS) studies. The UV–visible spectrum of the synthesized gold nanoparticles showed the surface plasmon resonance (SPR) at around 530 nm. The powder XRD pattern furnished evidence for the formation of face-centered cubic structure of gold having average crystallite size 5.9 nm. The TEM images showed the nanoparticles to be polydispersed, nearly spherical in shape and have sizes in the range 5–15 nm. The photoluminescence spectrum of the gold nanoparticles excited at 300 nm showed blue emission at around 440 nm. Gold nanoparticles loaded within the biomatrix studied using a modified 2,2-diphenyl-1-picrylhydrazyl (DPPH) method exhibited pronounced antioxidant activity.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

References

  1. 1.

    Zhou X, Wei Q, Sun K, Wang L (2009) Formation of ultrafine uniform gold nanoparticles by sputtering and redeposition. Appl Phys Lett 94:133107

  2. 2.

    Lucas BD, Kim JS, Chin C, Guo LJ (2008) Nanoimprint lithography based approach for the fabrication of large-area, uniformly-oriented plasmonic arrays. Adv Mater 20:1129–1134

  3. 3.

    Mishra YK, Avasthi DK, Kulriya PK, Singh F, Kabiraj D, Tripathi A, Pivin JC, Bayer IS, Biswas A (2007) Controlled growth of gold nanoparticles induced by ion irradiation: an in situ X-ray diffraction study. Appl Phys Lett 90:073110

  4. 4.

    Kabashin AV, Meunier M (2003) Synthesis of colloidal nanoparticles during femtosecond laser ablation of gold in water. J Appl Phys 94:7941–7943

  5. 5.

    Magnusson MH, Deppert K, Malm JO, Bovin JO, Samuelson L (1999) Gold nanoparticles: production, reshaping, and thermal charging. J Nanopart Res 1:243–251

  6. 6.

    Turkevich J (1985) Colloidal gold. Part I. Gold Bull 18:86–91

  7. 7.

    Brust M, Schiffrin DJ, Bethell D, Kiely CJ (1995) Novel gold-dithiol nano-networks with non-metallic electronic properties. Adv Mater 7:795–797

  8. 8.

    Gittins DI, Caruso F (2001) Spontaneous phase transfer of nanoparticulate metals from organic to aqueous media. Angew Chem Int Ed 40:3001–3004

  9. 9.

    De G, Bhattacharyya S (2008) Au nanoparticles in alumina sols and coatings. J Mater Chem 18:2816–2824

  10. 10.

    Polavarapu L, Xu QH (2009) A simple method for large scale synthesis of highly monodisperse gold nanoparticles at room temperature and their electron relaxation properties. Nanotechnology 20:185606–185612

  11. 11.

    Hu MS, Chen HL, Shen CH, Hong LS, Huang BR, Chen KH, Chen LC (2006) Photosensitive gold- nanoparticle-embedded dielectric nanowires. Nat Mater 5:102–106

  12. 12.

    Ghosh P, Han G, De M, Kim CK, Rotello VM (2008) Gold nanoparticles in delivery applications. Adv Drug Deliv Rev 60:1307–1315

  13. 13.

    Pingarron JM, Sedeno PY, Cortes AG (2008) Gold nanoparticle-based electrochemical biosensors. Electrochim Acta 53:5848–5866

  14. 14.

    Mikami Y, Dhakshinamoorthy A, Alvaroa M, García H (2013) Catalytic activity of unsupported gold Nanoparticles. Catal Sci Technol 3:58–69

  15. 15.

    Singh DK, Jagannathan R, Khandelwal P, Abraham PM, Poddar P (2013) In situ synthesis and surface functionalization of gold nanoparticles with curcumin and their antioxidant properties: an experimental and density functional theory investigation. Nanoscale 5:1882–1893

  16. 16.

    Zhao Y, Tian Y, Cui Y, Liu W, Ma W, Jiang X (2010) Small molecule-capped gold nanoparticles as potent antibacterial agents that target gram-negative bacteria. J Am Chem Soc 132:12349–12356

  17. 17.

    Nair B, Pradeep T (2002) Coalescence of nanoclusters and formation of submicron crystallites assisted by lactobacillus strains. Cryst Growth Des 2:293–298

  18. 18.

    Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Ramani R, Parischa R, Ajayakumar PV, Alam M, Sastry M, Kumar R (2001) Bioreduction of AuCl4 ions by the fungus, Verticillium sp. and surface trapping of the gold nanoparticles formed. Angew Chem Int Ed 40:3585–3588

  19. 19.

    Gardea-Torresdey JL, Parsons JG, Gomez E, Peralta-Videa J, Troiani HE, Santiago P, Jose Yacaman M (2002) Formation and growth of Au nanoparticles inside live alfalfa plants. Nano Lett 2:397–401

  20. 20.

    Shankar SS, Ahmad A, Pasricha R, Sastry M (2003) Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. J Mater Chem 13:1822–1826

  21. 21.

    Nayak D, Nag M, Banerjee S, Pal R, Laskar S, Lahiri S (2006) Preconcentration of 198Au in a green alga, Rhizoclonium. J Radioanal Nucl Chem 268:337–340

  22. 22.

    Yan L, Cai Y, Zheng B, Yuan H, Guo Y, Xiao D, Choi MMF (2012) Microwave-assisted synthesis of BSA- stabilized and HSA-protected gold nanoclusters with red emission. J Mater Chem 22:1000–1005

  23. 23.

    Roy S, Pauli G, Banerjee A (2012) The as-prepared gold cluster-based fluorescent sensor for the selective detection of AsIII ions in aqueous solution. Nanoscale 4:2734–2740

  24. 24.

    Devi PS, Banerjee S, Chowdhury SR, Kumar GS (2012) Eggshell membrane: a natural biotemplate to synthesize fluorescent gold nanoparticles. RSC Adv 2:11578–11585

  25. 25.

    Kennedy TAC, MacLean JL, Liu J (2012) Blue emitting gold nanoclusters templated by poly-cytosine DNA at low pH and poly-adenine DNA at neutral pH. Chem Commun 48:6845–6847

  26. 26.

    Xie J, Zheng Y, Ying JY (2009) Protein-directed synthesis of highly fluorescent gold nanoclusters. J Am Chem Soc 131:888–889

  27. 27.

    Liu CL, Wu HT, Hsiao YH, Lai CW, Shih CW, Peng YK, Tang KC, Chang HW, Chien YC, Hsiao JK, Cheng JT, Chou PT (2011) Insulin-directed synthesis of fluorescent gold nanoclusters: preservation of insulin bioactivity and versatility in cell imaging. Angew Chem Int Ed 50:7056–7060

  28. 28.

    Veeraapandian S, Sawant SN, Doble M (2012) Antibacterial and antioxidant activity of protein capped silver and gold nanoparticles synthesized with Escherichia coli. J Biomed Nanotechnol 8:140–148

  29. 29.

    Ramamurthy CH, Padma M, Daisy mariya samadanam I, Mareeswaran R, Suyavaran A, Suresh Kumar M, Premkumar K, Thirunavukkarasu C (2013) The extra cellular synthesis of gold and silver nanoparticles and their free radical scavenging and antibacterial properties. Colloids Surf B 102:808–815

  30. 30.

    Asmathunisha N, Kathiresan K (2013) A review on biosynthesis of nanoparticles by marine organisms. Colloids Surf B 103:283–287

  31. 31.

    Parial D, Patra HK, Dasgupta AK, Pal R (2012) Screening of different algae for green synthesis of gold nanoparticles. Eur J Phycol 47:22–29

  32. 32.

    Singaravelu G, Arockiamary JS, Kumar VG, Govindaraju K (2007) A novel extracellular synthesis of monodisperse gold nanoparticles using marine alga, Sargassum wightii Greville. Colloids Surf B 57:97–101

  33. 33.

    Mata YN, Torres E, Blazquez ML, Ballester A, Gonzalez F, Munoz JA (2009) Gold(III) biosorption and bioreduction with the brown alga Fucus vesiculosus. J Hazard Mater 166:612–618

  34. 34.

    Schrofel A, Kratosova G, Bohunicka M, Dobrocka E, Vavra I (2011) Biosynthesis of gold nanoparticles using diatoms-silica-gold and EPS-gold bionanocomposite formation. J Nanopart Res 13:3207–3216

  35. 35.

    Bhosale R, Rout J, Chaugule B (2012) The ethnobotanical study of an edible freshwater red alga, Lemanea fluviatilis (L.) C.Ag. from Manipur, India. Ethnobot Res Appl 10:69–76

  36. 36.

    Bajaj S, Vohora SB (2000) Anti-cataleptic, anti-anxiety and anti-depressant activity of gold preparations used in Indian systems of medicine. Indian J Pharmacol 32:339–346

  37. 37.

    Shah ZA, Vohora SB (2002) Antioxidant/restorative effects of calcined gold preparations used in Indian systems of medicine against global and focal models of ischaemia. Pharmacol Toxicol 90:254–259

  38. 38.

    Shah ZA, Gilani RA, Sharma P, Vohora SB (2005) Attenuation of stress-elicited brain catecholamines, serotonin and plasma corticosterone levels by calcined gold preparations used in Indian system of medicine. Basic Clin Pharmacol Toxicol 96:469–474

  39. 39.

    Edmonson WT (1992) Freshwater Biology. International Books and Periodicals Supply Service, New Delhi

  40. 40.

    Serpen A, Capuano E, Fogliano V, Gokmen V (2007) A new procedure to measure the antioxidant activity of insoluble food components. J Agric Food Chem 55:7676–7681

  41. 41.

    Richard JP (1978) The Chemistry of Gold. Elsevier, Amsterdam

  42. 42.

    Correa-Llanten DN, Munoz-Ibacache SA, Castro ME, Munoz PA, Blamey JM (2013) Gold nanoparticles synthesized by Geobacillus sp. strain ID17 a thermophilic bacterium isolated from Deception Island, Antarctica. Microb Cell Fact 12:75

  43. 43.

    Sarkar J, Roy SK, Laskar A, Chattopadhyay D, Acharya K (2013) Bioreduction of chloroaurate ions to gold nanoparticles by culture filtrate of Pleurotus sapidus Quel. Mater Lett 92:313–316

  44. 44.

    Boyd GT, Yu ZH, Shen YR (1986) Photoinduced luminescence from the noble metals and its enhancement on roughened surfaces. Phys Rev B 33:7923–7936

  45. 45.

    Dulkeith E, Niedereichholz T, Klar TA, Feldmann J, Von Plessen G, Gittins DI, Mayya KS, Caruso F (2004) Plasmon emission in photoexcited gold nanoparticles. Phys Rev B 70:205424

  46. 46.

    Wilcoxon JP, Martin JE, Parsapour F, Wiedenman B, Kelley DF (1998) Photoluminescence from nanosize gold clusters. J Chem Phys 108:9137–9143

  47. 47.

    Zheng J, Petty JT, Dickson RM (2003) High quantum yield blue emission from water-soluble Au8 nanodots. J Am Chem Soc 125:7780–7781

  48. 48.

    Zheng J, Zhang CW, Dickson RM (2004) Highly fluorescent, water-soluble, size-tunable gold quantum dots. Phys Rev Lett 93:077402

  49. 49.

    Philip D (2009) Biosynthesis of Au, Ag and Au–Ag nanoparticles using edible mushroom extract. Spectrochim Acta A 73:374–381

  50. 50.

    Naik GH, Priyadarsini KI, Satav JG, Banabalikar MM, Sohoni PP, Biyani MK, Mohan H (2003) Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry 63:97–104

  51. 51.

    Nie Z, Liu KJ, Zhong CJ, Wang LF, Yang Y, Tian Q, Liu Y (2007) Enhanced radical scavenging activity by antioxidant-functionalized gold nanoparticles: a novel inspiration for development of new artificial antioxidants. Free Radical Biol Med 43:1243–1254

  52. 52.

    Du L, Suo S, Wang G, Jia H, Liu KJ, Zhao B, Liu Y (2013) Mechanism and cellular kinetic studies of the enhancement of antioxidant activity by using surface-functionalized gold nanoparticles. Chem Eur J 19:1281–1287

  53. 53.

    Medhe S, Bansal P, Srivastava MM (2014) Enhanced antioxidant activity of gold nanoparticle embedded 3,6-dihydroxyflavone: a combinational study. Appl Nanosci 4:153–161

Download references

Acknowledgments

Authors thank SAIF, NEHU, Shillong, for TEM facilities. BS thanks University Grants Commission and MT thanks Department of Biotechnology, Government of India for Research Fellowships. We thank DBT e-Library Consortium (DeLCON) of Bioinformatics Centre, Assam University, Silchar.

Author information

Correspondence to Jayashree Rout.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Sharma, B., Purkayastha, D.D., Hazra, S. et al. Biosynthesis of fluorescent gold nanoparticles using an edible freshwater red alga, Lemanea fluviatilis (L.) C.Ag. and antioxidant activity of biomatrix loaded nanoparticles. Bioprocess Biosyst Eng 37, 2559–2565 (2014). https://doi.org/10.1007/s00449-014-1233-2

Download citation

Keywords

  • Biosynthesis
  • Gold nanoparticles
  • UV–visible
  • Photoluminescence
  • Antioxidant