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Preparation of eutectic substrate mixtures for enzymatic conversion of ATC to l-cysteine at high concentration levels

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Abstract

High concentration eutectic substrate solutions for the enzymatic production of l-cysteine were prepared. Eutectic melting of binary mixtures consisting of d,l-2-amino-Δ2-thiazoline-4-carboxylic acid (ATC) as a substrate and malonic acid occurred at 39 °C with an ATC mole fraction of 0.5. Formation of eutectic mixtures was confirmed using SEM, SEM–EDS, and XPS surface analyses. Sorbitol, MnSO4, and NaOH were used as supplements for the enzymatic reactions. Strategies for sequential addition of five compounds, including a binary ATC mixture and supplements, during preparation of eutectic substrate solutions were established. Eutectic substrate solutions were stable for 24 h. After 6 h of enzymatic reactions, a 550 mM l-cysteine yield was obtained from a 670 mM eutectic ATC solution.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2010-0022947).

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Correspondence to Chul Soo Shin.

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Youn, S.H., Park, H.W., Choe, D. et al. Preparation of eutectic substrate mixtures for enzymatic conversion of ATC to l-cysteine at high concentration levels. Bioprocess Biosyst Eng 37, 1193–1200 (2014). https://doi.org/10.1007/s00449-013-1090-4

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Keywords

  • l-Cysteine
  • ATC
  • Eutectic mixture
  • Malonic acid
  • Enzymatic reaction