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Continuous lipase-catalyzed esterification of soybean fatty acids under ultrasound irradiation

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Abstract

This work investigates the continuous production of alkyl esters from soybean fatty acid (FA) charges using immobilized Novozym 435 as catalyst. The experiments were performed in a packed-bed bioreactor evaluating the effects of FA charge to alcohol (methanol and ethanol) molar ratio, from 1:1 to 1:6, substrate flow rate in the range of 0.5–2.5 mL/min and output irradiation power up to 154 W, at fixed temperature of 65 °C, on the reaction conversion. Results showed that almost complete conversions to fatty acids ethyl esters were achieved at mild ultrasonic power (61.6 W), FA to ethanol molar ratio of 1:6, operating temperature (65 °C) and remained nearly constant for long-term reactions without negligible enzyme activity losses.

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Acknowledgments

The authors thank CNPq and Intecnial S.A. for the financial support and scholarships.

Author information

Correspondence to J. Vladimir Oliveira.

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Trentin, C.M., Scherer, R.P., Dalla Rosa, C. et al. Continuous lipase-catalyzed esterification of soybean fatty acids under ultrasound irradiation. Bioprocess Biosyst Eng 37, 841–847 (2014). https://doi.org/10.1007/s00449-013-1052-x

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Keywords

  • Esterification
  • Packed-bed reactor
  • Fatty acids
  • Lipase
  • Ultrasound