In this study, we developed recombinant Escherichia coli strains expressing Lactococcus lactis subsp. lactis Il1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinant E. coli XL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration of E. coli XL1-Blue expressing GadB from the OD600 of 2–10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore, E. coli XL1-Blue expressing GadB highly concentrated to the OD600 of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al2O3 as catalyst in toluene with the yield of 96 %.
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This work was supported by the R&D Program of MKE/KEIT (10033199 and 10033386) and a grant from the Next-Generation BioGreen 21 Program (SSAC, grant number: PJ008057), Rural Development Administration, Republic of Korea. Further support from Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the MEST (2012-0006693) is appreciated.
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Park, S.J., Kim, E.Y., Noh, W. et al. Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli . Bioprocess Biosyst Eng 36, 885–892 (2013). https://doi.org/10.1007/s00449-012-0821-2
- Gamma-aminobutyrate (GABA)
- Recombinant E. coli
- Glutamate decarboxylase
- Nylon 4