A neutralizing agent combination strategy was developed to enhance the succinic acid production by Actinobacillus succinogenes ATCC 55618. First, a maximal succinic acid production of 48.2 g/L was obtained at a culture pH of 7.5. Second, NaOH and KOH were screened to identify the optimal neutralizing agent for pH control. However, the production of succinic acid did not increase, and severe cell flocculation was observed due to a high concentration of metal ions when only one neutralizing agent was used to control pH. Finally, a neutralizing agent combination strategy was developed with a supply of neutralizing agents with OH− and carbonate. The cell flocculation was eliminated, and a maximum succinic acid production of 59.2 g/L was obtained with 5 M NaOH and 40 g/L of MgCO3; this production was 27.9% higher than that obtained with NaOH alone. The results obtained in this study may be useful for the large-scale industrial production of succinic acid.
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Financial support from the following funding agencies is gratefully acknowledged: the National Natural Science Foundation of China (NSFC, Project Nos. 20976038 and 21176059), the Key Project of Chinese Ministry of Education (Project No. 210132), the Hubei Provincial Natural Science Foundation for Innovative Research Team (Project No. 2008CDA002), the Scientific Research Key Project of Hubei Provincial Department of Education (Project No. Z20101401), the Discipline Leader Project of Wuhan Municipality (Project No. 200951830553), the Key Technology R&D Program of Wuhan Municipality (Project No. 201120822280-2), the Open Project Programs for the Key Laboratory of Fermentation Engineering (Ministry of Education), the National Key Laboratory of Biochemical Engineering (Project No. 2010KF-06), and the State Key Laboratory of Bioreactor Engineering. Ya-Jie Tang also thanks the Chutian Scholar Program (Hubei Provincial Department of Education, China) (2006).
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Wang, C., Zhu, L., Li, H. et al. Performance analyses of a neutralizing agent combination strategy for the production of succinic acid by Actinobacillus succinogenes ATCC 55618. Bioprocess Biosyst Eng 35, 659–664 (2012). https://doi.org/10.1007/s00449-011-0644-6
- Succinic acid
- Actinobacillus succinogenes ATCC 55618
- pH regulation
- Combined control strategy