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Culture medium containing glucose and glycerol as a mixed carbon source improves ε-poly-l-lysine production by Streptomyces sp. M-Z18


To improve the efficiency of ε-poly-l-lysine (ε-PL) production by Streptomyces sp. M-Z18, batch and fed-batch fermentations with glucose and glycerol (co-fermentations) were performed. The batch fermentations showed that the initial ratio of glucose to glycerol plays an important role in glucose/glycerol co-fermentation. The optimal glucose/glycerol weight ratio was 30/30; this resulted in a maximum ε-PL productivity of 5.26 g/L/d. Glucose and glycerol were consumed synergistically during the co-fermentation process, and the length of time during which the substrate was exhausted was significantly shortened compared with the single carbon source fermentation. Under optimized conditions, fed-batch fermentations with glucose and glycerol as a mixed carbon source achieved maximum ε-PL concentration and productivity values of 35.14 g/L and 4.85 g/L/d, respectively. These values were respectively 1.43- and 1.39-, and 1.17- and 1.16-folds higher than those obtained from fermentations with glucose and glycerol as single carbon sources. The present study is the first to suggest that glucose/glycerol co-fermentation may be an efficient strategy for ε-PL production by Streptomyces sp. M-Z18.

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This work was supported by a grant from Wuxi Science & Technology Support Program (CYE21N1107), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and 111 Project (111-2-06).

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Correspondence to Lei Tang or Zhong-Gui Mao.

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Chen, X., Ren, X., Dong, N. et al. Culture medium containing glucose and glycerol as a mixed carbon source improves ε-poly-l-lysine production by Streptomyces sp. M-Z18. Bioprocess Biosyst Eng 35, 469–475 (2012). https://doi.org/10.1007/s00449-011-0586-z

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  • ε-Poly-l-lysine
  • Glucose/glycerol co-fermentation
  • Streptomyces sp. M-Z18
  • Fed-batch fermentation