Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

A highly sensitive resonance scattering spectral assay for carcinoembryonic antigen using immunonanogold as probe and nanocatalyst of NH2OH–Cu(II) particle reaction

  • 126 Accesses

  • 4 Citations

Abstract

Nanogold of 10 nm was used to label carcinoembryonic antigen antibody (CEAAb) to prepare a probe (Au-CEAAb) for carcinoembryonic antigen (CEA). In a Na2HPO4–NaH2PO4 buffer solution of pH 6.8, CEA reacted with Au-CEAAb to form a big Au-CEAAb–CEA immunocomplex that can be removed by centrifugation. The unreacted Au-CEAAb in the centrifugal supernatant exhibited catalytic effect on the Cu2O particle reaction, and the Cu2O particles displayed a resonance scattering (RS) peak at 602 nm. When CEA increased, the RS intensity at 602 nm decreased, and the decreased RS intensity (ΔI 602 nm) was linear to CEA concentration (C CEA) in the range of 0.02–12 ng mL−1, with the regression equation of ΔI 602 nm = 27.1 C CEA + 3.3, correlation coefficient of 0.9978 and detection limit of 3 pg mL−1 CEA. The proposed method was applied to detect CEA in real samples, with satisfactory results.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

References

  1. 1.

    Luo HQ, Li NB, Liu SP (2006) Resonance Rayleigh scattering study of interaction of hyaluronic acid with ethyl violet dye and its analytical application. Biosens Bioelectron 21:1186–1194

  2. 2.

    Jiang ZL, Sun SJ, Liang AH (2006) Gold-labeled nanoparticle-based immunoresonance scattering spectral assay for trace apolipoprotein AI and apolipoprotein B. Clin Chem 52:1389–1394

  3. 3.

    Liu SP, Luo HQ, Li NB, Liu ZF, Zheng WX (2001) Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes. Anal Chem 73:3907–3914

  4. 4.

    Jiang ZL, Zou MJ, Liang AH (2008) An immunonanogold resonance scattering spectral probe for rapid assay of human chorionic gonadotrophin. Clin Chim Acta 387:24–30

  5. 5.

    Jiang ZL, Li JS, Wen GQ, Liu QY, Liang AH (2010) Nanocatalytic resonance scattering spectral analysis. Sci China Chem 53:1049–1054

  6. 6.

    Deng JY, Zhang SS, Jiang ZL, Liang AH (2008) Immunonanogold catalytic spectrophotometric determination of trace IgG. Spectrosc Spect Anal 28:2935–2938

  7. 7.

    Jiang ZL, Liao XJ, Deng AP (2008) Catalytic effect of nanogold on Cu(II)-N2H4 reaction and its application to resonance scattering immunoassay. Anal Chem 80:8681–8687

  8. 8.

    Wei XL, Zou MJ, Jiang ZL (2008) A highly sensitive resonance scattering assay for immunoglubin M using Ag(I)-hydroquinone–immunonanogold catalytic reaction. Plasmonics 3:73–78

  9. 9.

    Liu MY, Jia CP, Jin QH (2010) Novel colorimetric enzyme immunoassay for the detection of carcinoembryonic antigen. Talanta 81:1625–1629

  10. 10.

    He XL, Yuan R, Chai YQ (2008) A sensitive amperometric immunosensor for carcinoembryonic antigen detection with porous nanogold film and nano-Au/chitosan composite as immobilization matrix. J Biochem Biophys Methods 70:823–826

  11. 11.

    Yan J, Hu M, Li D, He Y, Zhao R, Jiang X, Song S, Wang L, Fan C (2008) A nano- and micro-integrated protein chip based on quantum dot probes and a microfluidic network. Nano Res 1:490–493

  12. 12.

    Shen GY, Lu JL (2010) Piezoelectric immunosensor based on gold nanoparticles capped with mixed self-assembled monolayer for detection of carcinoembryonic antigen. Thin Solid Films 518:5010–5013

  13. 13.

    Yuan YL, Yuan R, Chai YQ (2010) A novel label-free electrochemical immunosensor for carcinoembryonic antigen detection based on the [Ag–Ag2O]/SiO2 nanocomposite material as a redox probe. J Electroanal Chem 643:15–19

Download references

Acknowledgments

This work supported by the National Natural Science Foundation of China (Nos. 20865002, 20965002) and the Natural Science Foundation of Guangxi (Nos. 0832260, 0991021Z).

Author information

Correspondence to Aihui Liang or Zhiliang Jiang.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Liu, Q., Wen, G., Liang, A. et al. A highly sensitive resonance scattering spectral assay for carcinoembryonic antigen using immunonanogold as probe and nanocatalyst of NH2OH–Cu(II) particle reaction. Bioprocess Biosyst Eng 34, 499–504 (2011). https://doi.org/10.1007/s00449-010-0494-7

Download citation

Keywords

  • CEA
  • Immunonanogold catalysis
  • Cu2O particle
  • Resonance scattering spectral assay