Batch fermentative production of welan gum by Alcaligenes sp. CGMCC2428 was investigated under various oxygen supply conditions using regulating agitation speed. Based on a three kinetic parameters analysis that includes specific cell growth rate (μ), specific glucose consumption rate (q s), and specific welan formation rate (q p), a two-stage agitation speed control strategy was proposed to achieve high concentration, high yield, and high viscosity of welan. During the first 22 h, the agitation speed in 7.5 L fermenter was controlled at 800 rpm to maintain high μ for cell growth. The agitation was then reduced step-wise to 600 rpm to maintain a changing profile with stable dissolved oxygen levels and obtain high qp for high welan accumulation. Finally, the maximum concentration of welan was reached at 26.3 ± 0.89 g L−1 with a yield of 0.53 ± 0.003 g g−1 and the welan gum viscosity of 3.05 ± 0.10 Pa s, which increased by an average of 15.4, 15.2, and 20.1% over the best results controlled by constant agitation speeds.
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This work was supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 08KJA180001) and Jiangsu Key Technology Research and Development Program (No. BE2009363).
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Li, H., Xu, H., Xu, H. et al. Enhanced welan gum production using a two-stage agitation speed control strategy in Alcaligenes sp. CGMCC2428. Bioprocess Biosyst Eng 34, 95–102 (2011). https://doi.org/10.1007/s00449-010-0450-6
- Welan gum
- Alcaligenes sp. CGMCC2428
- Agitation speed
- Two-stage control strategy
- Batch fermentation