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Lactic acid production directly from starch in a starch-controlled fed-batch operation using Lactobacillus amylophilus

Abstract

Based on the batch results, we constructed a simplified simultaneous saccharification and fermentation (SSF) model for the simulation of lactic acid production directly from unhydrolyzed potato starch using Lactobacillus amylophilus. The results of batch operation at different initial starch concentrations (20, 40 and 60 g/l) indicated that a higher initial starch concentration would lead to a slightly lower productivity, but would largely decrease the yield. Among that, the batch with 20 g/l of initial starch had the maximum productivity and the maximum yield, which would be 0.31 g/(l h) and 98% (g/g), respectively. In view of increasing the productivity and the final lactic acid concentration, a starch-controlled fed-batch operation with 20 g/l of initial starch was performed. It showed the fed-batch operation with starch controlled at 8 ± 1 g/l by adjusting the starch-feeding rate led to the maximum productivity of 0.75 g/(l h) and the yield of 69%.

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Abbreviations

μ m :

Maximum-specific growth rate (1/h).

K L :

Lactic acid inhibition constant for fermentation (g/l).

K S :

Glucose saturation constant (g/l).

K I :

Glucose inhibition constant for fermentation (g/l).

V m :

Maximum saccharification rate from starch, g/(l h).

K m :

Michaelis–Menten constant of starch (g/l).

\( K_{\text{G}} \) :

Glucose inhibition constant for saccharification from starch (g/l)

K R :

Reducing sugars inhibition constant for saccharification from starch (g/l)

\( V_{\text{mR}} \) :

Maximum saccharification from reducing sugars [g/(l h)]

\( K_{\text{mR}} \) :

Michaelis–Menten constant of reducing sugars (g/l)

\( K_{\text{GR}} \) :

Glucose inhibition constant for saccharification from reducing sugars (g/l)

\( {\text{Ratio}} \) :

The ratio of starch converted to glucose and starch converted to reducing sugar

α :

Growth-associated parameter for lactic acid production (g P/g X)

β :

Non-growth-associated parameter for lactic acid production (g P/g X/h)

γ :

Growth-associated parameter for glucose depletion (g G/g X)

δ :

Non-growth-associated parameter for glucose depletion (g G/g X/h)

K d :

Specific cell death rate (1/h)

X m :

Maximum cell concentration (g/l)

G*:

Virtual glucose concentration produced (g/l)

X :

Cell concentration (g/l)

P :

Lactic acid production concentration (g/l)

G :

Glucose concentration (g/l)

R :

Reducing sugars concentration (g/l)

S :

Starch concentration (g/l)

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Acknowledgments

The authors wish to thank the National Science Council of the R.O.C. for financial supports (NSC 97-2221-E-029-005).

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Correspondence to Hong-Wei Yen.

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Yen, H., Kang, J. Lactic acid production directly from starch in a starch-controlled fed-batch operation using Lactobacillus amylophilus . Bioprocess Biosyst Eng 33, 1017–1023 (2010). https://doi.org/10.1007/s00449-010-0426-6

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Keywords

  • Amylolytic
  • SSF
  • Starch controlled
  • Lactobacillus amylophilus
  • Fed-batch