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Lactic acid production directly from starch in a starch-controlled fed-batch operation using Lactobacillus amylophilus


Based on the batch results, we constructed a simplified simultaneous saccharification and fermentation (SSF) model for the simulation of lactic acid production directly from unhydrolyzed potato starch using Lactobacillus amylophilus. The results of batch operation at different initial starch concentrations (20, 40 and 60 g/l) indicated that a higher initial starch concentration would lead to a slightly lower productivity, but would largely decrease the yield. Among that, the batch with 20 g/l of initial starch had the maximum productivity and the maximum yield, which would be 0.31 g/(l h) and 98% (g/g), respectively. In view of increasing the productivity and the final lactic acid concentration, a starch-controlled fed-batch operation with 20 g/l of initial starch was performed. It showed the fed-batch operation with starch controlled at 8 ± 1 g/l by adjusting the starch-feeding rate led to the maximum productivity of 0.75 g/(l h) and the yield of 69%.

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μ m :

Maximum-specific growth rate (1/h).

K L :

Lactic acid inhibition constant for fermentation (g/l).

K S :

Glucose saturation constant (g/l).

K I :

Glucose inhibition constant for fermentation (g/l).

V m :

Maximum saccharification rate from starch, g/(l h).

K m :

Michaelis–Menten constant of starch (g/l).

\( K_{\text{G}} \) :

Glucose inhibition constant for saccharification from starch (g/l)

K R :

Reducing sugars inhibition constant for saccharification from starch (g/l)

\( V_{\text{mR}} \) :

Maximum saccharification from reducing sugars [g/(l h)]

\( K_{\text{mR}} \) :

Michaelis–Menten constant of reducing sugars (g/l)

\( K_{\text{GR}} \) :

Glucose inhibition constant for saccharification from reducing sugars (g/l)

\( {\text{Ratio}} \) :

The ratio of starch converted to glucose and starch converted to reducing sugar

α :

Growth-associated parameter for lactic acid production (g P/g X)

β :

Non-growth-associated parameter for lactic acid production (g P/g X/h)

γ :

Growth-associated parameter for glucose depletion (g G/g X)

δ :

Non-growth-associated parameter for glucose depletion (g G/g X/h)

K d :

Specific cell death rate (1/h)

X m :

Maximum cell concentration (g/l)


Virtual glucose concentration produced (g/l)

X :

Cell concentration (g/l)

P :

Lactic acid production concentration (g/l)

G :

Glucose concentration (g/l)

R :

Reducing sugars concentration (g/l)

S :

Starch concentration (g/l)


  1. 1.

    Datta R, Henry M (2006) Lactic acid: recent advances in products, processes and technologies—a review. J Chem Technol Biotechnol 81:1119–1126

  2. 2.

    Reddy G, Altaf M, Maveena BJ, Venkateshwar M, Kumar EV (2008) Amylolytic bacterial lactic acid fermentation- a review. Biotechnol Adv 28:22–34

  3. 3.

    Altaf M, Venkateshwar M, Srijana M, Reddy G (2007) An economic approach for L-(+) lactic acid fermentation by Lactobacillus amylophilus GV6 using inexpensive carbon and nitrogen sources. J Appl Microbiol 103:372–380

  4. 4.

    Vishnu C, Naveena BJ, Altaf M, Venkateshwar M, Reddy G (2006) Amylopullulanase-A novel enzyme of L. amylophilus GV6 in direct fermentation of starch to L(+) lactic acid. Enzyme Microb Technol 38:545–550

  5. 5.

    Yumoto I, Ikeda K (1995) Direct fermentation of starch to l-(+)-lactic acid using Lactobacillus amylophilus. Biotechnol Lett 17:543–546

  6. 6.

    Zhang DX, Cheryan M (1991) Direct fermentation of starch to lactic acid by Lactobacillus amylophilus. Biotechnol Lett 13:733–738

  7. 7.

    Nakamura LK, Crowell CD (1979) Lactobacillus amylophilus, a new starch-hydrolyzing species from swine waste-corn fermentation. Dev Ind Microbiol 20:532–540

  8. 8.

    Ding S, Tan T (2006) l-Lactic acid production by Lactobacillus casei fermentation using different fed-batch feeding strategies. Process Biochem 41:1451–1454

  9. 9.

    Roy S, Gudi RD, Venkatesh KV, Shah SS (2001) Optimal control strategies for simultaneous saccharification and fermentation of starch. Process Biochem 36:713–722

  10. 10.

    Anuradha R, Suresh AK, Venkatesh KV (1999) Simultaneous saccharification and fermentation of starch to lactic acid. Process Biochem 35:367–377

  11. 11.

    Burgos-Rubio CNN, Okos MR, Wankat PC (2000) Kinetic study of the conversion of different substrates to lactic acid using Lactobacillus bulgaricus. Biotechnol Prog 16:305–314

  12. 12.

    Luo J, Xia L, Lin J, Cen P (1997) Kinetics of simultaneous saccharification and lactic acid fermentation processes. Biotechnol Prog 13:762–767

  13. 13.

    Philippidis GP, Hatzis C (1997) Biochemical engineering analysis of critical process factors in the biomass-to-ethanol technology. Biotechnol Prog 13:222–231

  14. 14.

    Zhang ZY, Jin B, Kelly JM (2007) Production of lactic acid and byproducts from waste potato starch by Rhizopus arrhizus: role of nitrogen sources. World J Microbiol Biotechnol 23:229–236

  15. 15.

    Kimura S, Konagata A (2003) A genetic algorithm with distance independent diversity control for high dimensional function optimization. Trans Jpn Soc Artificial Intel 18:193–202

  16. 16.

    Xiao Z, Storms R, Tsang A (2006) A quantitative starch-iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem 35:146–148

  17. 17.

    Luedeking R, Piret EL (1959) A kinetic study of the lactic acid fermentation: batch process at controlled pH. J Biochem Microbiol Technol Eng 1:393–412

  18. 18.

    Yen HW, Lee YC (2009) Production of lactic acid from raw sweet potato powders by Rhizopus oryzae immobilized in sodium alginate apsules. Appl Biochem Biotechnol. doi:10.1007/s12010-009-8884-5

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The authors wish to thank the National Science Council of the R.O.C. for financial supports (NSC 97-2221-E-029-005).

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Correspondence to Hong-Wei Yen.

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Yen, H., Kang, J. Lactic acid production directly from starch in a starch-controlled fed-batch operation using Lactobacillus amylophilus . Bioprocess Biosyst Eng 33, 1017–1023 (2010).

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  • Amylolytic
  • SSF
  • Starch controlled
  • Lactobacillus amylophilus
  • Fed-batch