To construct a novel simultaneous SPR and QCM sensing system, an AT-cut quartz crystal is fabricated by sputtering 250 nm of ITO on one side of the quartz plate over a 5-nm thick underlay of titanium, while a 50-nm thick layer of gold is sputter-deposited on the other side to induce a total light reflection of an incident laser beam on the thin gold layer. The signals of the sensing system are detected using a Handy-SPR and QCA922 when dropping 200 μL of Milli-Q water into the sensing cell. A decrease in the SPR reflected light intensity is clearly identified. In the same experiment, the changes in the resonant frequency and resistance are about 2 kHz and 200 Ω, respectively. Furthermore, the oscillation stabilities of the resonant frequency and resistance are about 50 Hz and 2 Ω, respectively, which are sufficient to detect a large mass change on the QCM/SPR chip.
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This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (KOSEF, 2009-0064245).
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Kim, J., Kim, S., Ohashi, T. et al. Construction of simultaneous SPR and QCM sensing platform. Bioprocess Biosyst Eng 33, 39 (2010). https://doi.org/10.1007/s00449-009-0370-5
- Resonant frequency
- Resonant resistance
- SPR angle
- Index matching oil