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Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057)

Abstract

An endosymbiotic Pseudomonas sp. (MSI057), which could produce high yields of lipase, was isolated from marine sponge Dendrilla nigra, collected from the peninsular coast of India. Maximum production of enzyme was obtained in minimal medium supplemented with 1% tributyrin. Catabolite repression was observed when the medium was supplemented with readily available carbon sources. The optimum temperature and pH for the enzyme production was 30 °C and 9.0, respectively. The enzyme exhibited maximum activity in pH range of 8–9 with an optimum pH 9.0. The activity of purified enzyme was optimum at 37 °C and showed 80% activity at 20 °C and the enzyme activity decreased dramatically above 50 °C. Based on the present findings, the enzyme was characterized as psychrophilic alkaline lipase, which can be developed for industrial applications.

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Acknowledgments

We wish to thank Dr. A. P. Lipton, Professor and Head, Department of Marine Biotectnology, CMST, Rajakkamangalam, Nagercoil for valuable suggestions throughout this study. J.S. is thankful to DST (SR/FT/L-19/2004), New Delhi and International Foundation for Science (RGA No. F/3776-1), Sweden for financial support.

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Correspondence to Joseph Selvin.

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Kiran, G.S., Shanmughapriya, S., Jayalakshmi, J. et al. Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057). Bioprocess Biosyst Eng 31, 483–492 (2008). https://doi.org/10.1007/s00449-007-0186-0

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Keywords

  • Extracellular lipase
  • Sponge endosymbionts
  • Pseudomonas
  • Optimization
  • Psychrophilic lipase