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Comparative study of thermostabilty and ester synthesis ability of free and immobilized lipases on cross linked silica gel

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Abstract

A novel support has been utilized for immobilization of lipase, which was prepared by amination of silica with ethanolamine followed by cross linking with glutaraldehyde. Lipases from Rhizopus oryzae3562 and Enterobacter aerogenes were immobilized on activated silica gel, where they retained 60 and 50% of respective original activity. The thermal stability of the immobilized lipases was significantly improved in comparison to the free forms while the pH stability remained unchanged. E. aerogenes and R. oryzae3562 lipases retained 75 and 97% of respective initial activity on incubation at 90 °C, whereas both the free forms became inactive at this temperature. The conversion yield of isoamyl acetate was found to be higher with the immobilized fungal (90 vs. 21%) and bacterial lipases (64 vs. 18%) than the respective free forms. Immobilized R. oryzae3562 lipases retained 50% activity for isoamyl acetate synthesis up to ten cycles whereas it was eight cycles for E. aerogenes.

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Acknowledgments

The authors wish to acknowledge CSIR, Government of India and Department of Biotechnology, India for providing research fellowship to Annapurna Kumari and Paramita Mahapatra, respectively.

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Correspondence to Rintu Banerjee.

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Kumari, A., Mahapatra, P., Kumar, G.V. et al. Comparative study of thermostabilty and ester synthesis ability of free and immobilized lipases on cross linked silica gel. Bioprocess Biosyst Eng 31, 291–298 (2008). https://doi.org/10.1007/s00449-007-0160-x

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Keywords

  • Immobilized lipases
  • Silica
  • Themostability
  • Rhizopus oryzae3562
  • Enterobacter aerogenes