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Butanol production from wheat straw hydrolysate using Clostridium beijerinckii


In these studies, butanol (acetone butanol ethanol or ABE) was produced from wheat straw hydrolysate (WSH) in batch cultures using Clostridium beijerinckii P260. In control fermentation 48.9 g L−1 glucose (initial sugar 62.0 g L−1) was used to produce 20.1 g L−1 ABE with a productivity and yield of 0.28 g L−1 h−1 and 0.41, respectively. In a similar experiment where WSH (60.2 g L−1 total sugars obtained from hydrolysis of 86 g L−1 wheat straw) was used, the culture produced 25.0 g L−1 ABE with a productivity and yield of 0.60 g L−1 h−1 and 0.42, respectively. These results are superior to the control experiment and productivity was improved by 214%. When WSH was supplemented with 35 g L−1 glucose, a reactor productivity was improved to 0.63 g L−1 h−1 with a yield of 0.42. In this case, ABE concentration in the broth was 28.2 g L−1. When WSH was supplemented with 60 g L−1 glucose, the resultant medium containing 128.3 g L−1 sugars was successfully fermented (due to product removal) to produce 47.6 g L−1 ABE, and the culture utilized all the sugars (glucose, xylose, arabinose, galactose, and mannose). These results demonstrate that C. beijerinckii P260 has excellent capacity to convert biomass derived sugars to solvents and can produce over 28 g L−1 (in one case 41.7 g L−1 from glucose) ABE from WSH. Medium containing 250 g L−1 glucose resulted in no growth and no ABE production. Mixtures containing WSH + 140 g L−1 glucose (total sugar approximately 200 g L−1) showed poor growth and poor ABE production.

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N. Qureshi would like to thank Professor David Jones (University of Otago, Dunedin, New Zealand) for his generous gift of C. beijerinckii P260. N. Qureshi would also like to thank Marsha Ebener for reading and formatting this manuscript. Help from John Michael Henderson, Mark Maroon, and Gregory Kennedy for preparing WSH and conducting some of the experiments is gratefully acknowledged.

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Correspondence to Nasib Qureshi.

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Mention of trade names or commercial products in this article is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the United States Department of Agriculture.

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Qureshi, N., Saha, B.C. & Cotta, M.A. Butanol production from wheat straw hydrolysate using Clostridium beijerinckii . Bioprocess Biosyst Eng 30, 419–427 (2007). https://doi.org/10.1007/s00449-007-0137-9

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  • Wheat straw hydrolysate (WSH)
  • Clostridium beijerinckii
  • Butanol or ABE
  • Productivity
  • Yield