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Continuous ethanol fermentation of cheese whey powder solution: effects of hydraulic residence time

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Abstract

Continuous ethanol fermentation of cheese whey powder solution was realized using pure culture of Kluyveromyces marxianus (DSMZ 7239) at hydraulic residence times (HRT) between 12.5 and 60 h. Sugar utilization, ethanol and biomass formation were investigated as functions of HRT. Effluent sugar concentration decreased, but percent sugar utilization, ethanol and biomass concentrations increased with HRT. Ethanol productivity was maximum (0.745 gE l−1h−1) at an HRT of 43.2 h where the biomass productivity was almost minimum (0.18 gX l−1 h−1). The ethanol yield coefficient was almost constant at 0.4 gE g−1S up to HRT of 43.2 h and the growth yield coefficient was minimum at HRT of 43.2 h. Kinetic models were developed and the constants were determined by using the experimental data.

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References

  1. 1.

    Grba S, Tomas VS, Stanzer D, Vahcic N, Skrlin A (2002) Selection of yeast strain Kluyveromyces marxianus for alcohol and biomass production on whey. Chem Biochem Eng Q 16:13–16

  2. 2.

    Siso MIG (1996) The Biotechnological of utilization of cheese whey: a review. Bioresour Technol 57:1–11

  3. 3.

    Domingues L, Lima N, Teixeira JA (2001) Alcohol production from cheese whey permeate using genetically modified flocculent yeast cells. Biotechnol Bioeng 72:507–514

  4. 4.

    Silveira WB, Passos FJV, Mantovani HC, Passos FML (2005) Ethanol production from cheese whey permeate by Kluyveromyces marxianus UFV-3: a flux analysis of oxido-reductive metabolism as a function of lactose concentration and oxygen levels. Enzyme Microb Technol 36:930–936

  5. 5.

    Kourkoutas Y, Dimitropoulou S, Kanellaki M, Marchant R, Nigam P, Banat IM, Koutinas AA (2002) High-temperature alcoholic fermentation of whey using Kluyveromyces marxianus IMB3 yeast immobilized on delignified cellulosic material. Bioresour Technol 82:177–181

  6. 6.

    Kourkoutas Y, Psarianos C, Koutinas AA, Kanellaki M, Banat IM, Marchant R (2002) Continuous whey fermentation using kefir yeast immobilized on delignified cellulosic material. J Agric Food Chem 50:2543–2547

  7. 7.

    Zafar S, Owais M (2006) Ethanol production from crude whey by Kluyveromyces marxianus Biochem. Eng J 27:295–298

  8. 8.

    Guadix A, Sorenson E, Papageorgiou LG, Guadix EM (2004) Optimal design and operation of ultrafiltration plants. J Memb Sci 235:131–138

  9. 9.

    Ghaly AE, El-Taweel AA (1995) Effect of micro-aeration on the growth of Candida pseudotropicalis and production of ethanol during batch fermentation of cheese whey. Bioresour Technol 52:203–217

  10. 10.

    Ghaly AE, El-Taweel AA (1997) Kinetic modelling of continuous production of ethanol from cheese whey. Biomass Bioenergy 12:461–472

  11. 11.

    Banat IM, Nigam P, Marchant R (1992) Isolation of thermotolerant fermentative yeast capable of growth at 52°C and ethanol production at 45°C and 50°C. World J Microb Biotechnol 8:259–263

  12. 12.

    Banat IM, Marchant R (2005) Characterization and potential industrial applications of five novel, thermotolerant, fermentative yeast strains. World J Microb Biotechnol 11:304–306

  13. 13.

    Banat IM, Singh D, Marchant R (1996) The use of a thermotolerant fermentative Kluyveromyces marxianus IMB 3 yeast strain for ethanol production. Acta Biotechnol 16:215–223

  14. 14.

    Brady D, Nigam P, Marchant R, McHale AP (1997) Ethanol production at 45°C by alginate-immobilized Kluyveromyces marxianus IMB3 during growth on lactose-containing media. Bioprocess Eng 16:101–104

  15. 15.

    Longhi LGS, Luvizetto DJ, Ferreira LS, Rech R, Ayub MAZ, Secchi AR (2004) A growth kinetic model of Kluyveromyces marxianus cultures on cheese whey as a substrate. J Indus Microb Biotechnol 31:35–40

  16. 16.

    Kargi F, Ozmihci S (2006) Utilization of cheese whey powder for ethanol fermentations: effects of operating conditions. Enzyme Microb Technol 38:711–718

  17. 17.

    Shuler ML, Kargi F (2002) Bioprocess engineering: basic concepts, 2nd edn. Prentice Hall, USA

  18. 18.

    Bailey JE, Ollis DF (1986) Biochemical engineering fundamentals, 2nd edn. McGraw Hill, USA

  19. 19.

    Oliveire SC, Paiva TCB, Visconti AES, Giudici R (1999) Continuous alcoholic fermentation process: model considering loss of cell viability. Bioprocess Eng 20:157–160

  20. 20.

    Oliveira SC, DeCastro HF, Visconti AES, Giudici R (1999) Continuous ethanol fermentation in a tower reactor with flocculating yeast recycle: scale—up effects on process performance, kinetic parameters and model predictions. Bioprocess Eng 20:525–530

  21. 21.

    Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 8:350–366

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Acknowledgment

This study was supported by the funds from the State Planning Organization, Ankara and also Dokuz Eylul University, Izmir, Turkey.

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Correspondence to Fikret Kargi.

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Ozmihci, S., Kargi, F. Continuous ethanol fermentation of cheese whey powder solution: effects of hydraulic residence time. Bioprocess Biosyst Eng 30, 79–86 (2007). https://doi.org/10.1007/s00449-006-0101-0

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Keywords

  • Cheese whey powder (CWP)
  • Ethanol formation
  • Kluyveromyces marxianus
  • Continuous fermentation