To maximize nitrogen utilization rates during nitrification and denitrification in a simultaneous reaction for direct nitrogen removal from ammonia–nitrogen in a single reactor, two different carriers were applied that immobilized nitrifiers and denitrifiers separately. With the optimized DO concentration and mixing ratio of immobilization carriers, ammonium–nitrogen was successfully removed as designed until the middle phase of treatment where nitrogen removal rate was higher than 83% of the theoretical value, although an imbalance between nitrification and denitrification occurred at a later phase of treatment where residual nitrate–nitrogen concentration was less than 2 mg/l. The new approach using two different carriers to immobilize nitrifiers and denitrifiers separately was proved useful for controlling both nitrification and denitrification rates, enabling the utilization of maximum treatment ability of both nitrifiers and denitrifiers in a single reactor for direct nitrogen removal from ammonium–nitrogen.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Wiesmann U (1994) Biological nitrogen removal from wastewater. Adv Biochem Eng Biotechnol 51:114–154
Masuda S, Watanabe Y, Ishigoro M (1991) Biofilm properties and simultaneous nitrification and denitrification in aerobic rotating biological contactors. Water Sci Technol 23:1355–1363
Dos Santos VMPM, Bruijnse M, Tramper J, Wijffels RH (1996) The magic-beads concept: an integrated approach to nitrogen removal with co-immobilized microorganisms. Appl Microbiol Biotechnol 45:447–453
Kokufuta E, Shimohashi M, Nakamura I (1988) Simultaneously occurring nitrification and denitrification under oxygen gradient by polyelectrolyte complex-coimmobilized Nitrosomonas europaea and Paracoccus denitrificans cells. Biotechnol Bioeng 31:382–384
Matsumura M, Yamamoto T, Wang P, Shinabe K, Yasuda K (1997) Rapid nitrification with immobilized cell using macro-porous cellulose carrier. Water Res 31:1027–1034
Matsumura M, Tsubota H, Ito O, Wang P, Yasuda K (1997) Development of bioreactors for denitrification with immobilized cells. J Ferment Bioeng 84:144–150
This work was partially supported by BRAIN (Bio-Oriented Technology Research Advancement Institution), Japan.
About this article
Cite this article
Nakano, K., Iwasawa, H., Ito, O. et al. Improved simultaneous nitrification and denitrification in a single reactor by using two different immobilization carriers with specific oxygen transfer characteristics. Bioprocess Biosyst Eng 26, 141–145 (2004). https://doi.org/10.1007/s00449-003-0345-x
- Simultaneous nitrification and denitrification