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Effect of phosphate concentration on the production of dextransucrase by Leuconostoc mesenteroides NRRL B512F

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Abstract

Leuconostoc mesenteroides NRRL B512F is the main strain used in industrial fermentations to produce dextransucrase and dextran. This process has been studied since the Second World War, when it was used as blood plasma expander. A study about the effect of phosphate concentration on cell propagation in a semicontinuous shake-flask culture is described in this work. Dextransucrase is obtained by fermentation of the Leuconostoc mesenteroides NRRL B512F in the presence of sucrose as substrate, a nitrogen source (corn liquor or yeast extract) and minerals. Phosphate is currently used in order to buffer the culture medium. Cell propagation can be done through a repeated batch culture, where dilution in a fresh medium is made with relatively short periods. The standard medium for dextransucrase production is prepared using 0.1 M of K2HPO4. In this work the level of phosphate was increased to 0.3 M, and an increase on biomass and on the enzyme activity was found when phosphate enriched medium was used. Higher phosphate buffer concentration was also able to keep the pH values above 5.0 during the entire process, avoiding enzyme denaturation.

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Acknowledgements

To the Brazilian Funding Angencies: CNPQ, CAPES and FAEP. This paper was accepted and presented at ESBES-4.

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Correspondence to S. Rodrigues.

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Rodrigues, S., Lona, L.M.F. & Franco, T.T. Effect of phosphate concentration on the production of dextransucrase by Leuconostoc mesenteroides NRRL B512F. Bioprocess Biosyst Eng 26, 57–62 (2003). https://doi.org/10.1007/s00449-003-0330-4

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Keywords

  • Dextransucrase
  • Shake-flask fermentation
  • Leuconostoc mesenteroides NRRL B512F