Linking nutrient stoichiometry to Zika virus transmission in a mosquito
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Food quality and quantity serve as the basis for cycling of key chemical elements in trophic interactions; yet the role of nutrient stoichiometry in shaping host–pathogen interactions is under appreciated. Most of the emergent mosquito-borne viruses affecting human health are transmitted by mosquitoes that inhabit container systems during their immature stages, where allochthonous input of detritus serves as the basal nutrients. Quantity and type of detritus (animal and plant) were manipulated in microcosms containing newly hatched Aedes aegypti mosquito larvae. Adult mosquitoes derived from these microcosms were allowed to ingest Zika virus-infected blood and then tested for disseminated infection, transmission, and total nutrients (percent carbon, percent nitrogen, ratio of carbon to nitrogen). Treatments lacking high-quality animal (insect) detritus significantly delayed development. Survivorship to adulthood was closely associated with the amount of insect detritus present. Insect detritus was positively correlated with percent nitrogen, which affected Zika virus infection. Disseminated infection and transmission decreased with increasing insect detritus and percent nitrogen. We provide the first definitive evidence linking nutrient stoichiometry to arbovirus infection and transmission in a mosquito using a model system of invasive Ae. aegypti and emergent Zika virus.
KeywordsNutrition Ontogeny Infection Zika virus
An isolate of Zika virus was kindly provided by the Centers for Disease Control and Prevention. We thank A. Carels, B. Eastmond, S. Ortiz, K. Wiggins, and R. Zimler for technical support with experiments. K. Kuehn provided technical support for nutrient analysis of mosquitoes.
Author contribution statement
DAY and BWA conceived of and designed the project. ASP and SKB carried out mosquito husbandry, viral propagation, molecular work and collected project data. CLD performed the nutrient analyses and mosquito mass measurements. DAY analyzed and interpreted the data with BWA. ASP, BWA, DAY and CLD wrote and edited the manuscript. All authors gave final approval for manuscript publication.
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