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Evaluating local adaptation of a complex phenotype: reciprocal tests of pigmy rattlesnake venoms on treefrog prey

Abstract

Theory predicts that predator–prey interactions can generate reciprocal selection pressures on species pairs, which can result in local adaptation, yet the presence and pattern of local adaptation is poorly studied in vertebrate predator–prey systems. Here, we used a reciprocal common garden (laboratory) experimental design involving comparisons between local and foreign populations to determine if local adaptation was present between a generalist predator—the pigmy rattlesnake (Sistrurus miliarius)—and a co-occurring prey—the squirrel treefrog (Hyla squirella). We conducted toxicity trials using snake venom from two populations separated by 340 km tested on prey from sympatric and allopatric populations, resulting in data from four venom origin–frog origin combinations. We assessed venom effectiveness using two measures (frog mortality at 24 h and time to frog death) and then used regression analyses to look for a signal of local adaptation with either measure. We found evidence for local adaptation for one measure (time to death), but not the other (frog mortality). We argue that in this system, the time to death of a prey item is a more ecologically relevant measure of venom effectiveness than is frog mortality at 24 h. Our results document an example of local adaptation between two interacting vertebrates using a whole-organism assay and a local versus foreign criteria and provide evidence that population-level variation in snake venom is adaptive.

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Acknowledgements

We thank Hannah VanSumeren for her assistance with lab work. S. Mackessy provided helpful advice for the development of LD50 assay protocols. We also thank numerous students, visiting researchers, and collaborators for help locating snakes in the field including C. Lind, J. Serrao, M. Pilgrim, E. Royal, D. Rokyta, M. Margres, K. Wray, M. Holding, R. Denton, and D. Salazar-Valenzuela. Our manuscript was improved, thanks to comments from G. Zancolli and one anonymous reviewer. Venoms from pigmy rattlesnakes were collected under a License to Possess or Exhibit Venomous Reptiles and/or Reptiles of Concern from the Florida Fish and Wildlife Conservation Commission with the following license renewal identifications (year): 411-75193 (2011–2012), 411-95180 (2012–2013), 411-104419 (2013–2014), and 411-115575 (2014–2015). Squirrel treefrogs were obtained under a Scientific Collecting Permit from the Florida Fish and Wildlife Conservation Commission (permit number LSSC-11-00067A).

Author information

SASW and HLG conceived the study. SASW and TMF collected organisms in the field and performed statistical analyses. SASW conducted laboratory experiments and wrote the manuscript. All authors were involved in manuscript revisions.

Correspondence to Sarah A. Smiley-Walters.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable institutional and/or national guidelines for the care and use of animals were followed. Research methods were approved by the Stetson University IACUC (protocol #2011TF101).

Funding

This research was made possible by financial support from The Brown Visiting Teacher-Scholar Fellows Program at Stetson University.

Additional information

We document local adaptation of a complex trait in a predator–prey interaction between two vertebrates. Our study demonstrates that population-level variation in snake venom has adaptive significance.

Communicated by Lin Schwarzkopf.

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Smiley-Walters, S.A., Farrell, T.M. & Gibbs, H.L. Evaluating local adaptation of a complex phenotype: reciprocal tests of pigmy rattlesnake venoms on treefrog prey. Oecologia 184, 739–748 (2017). https://doi.org/10.1007/s00442-017-3882-8

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Keywords

  • Predator–prey interactions
  • Fitness trade-off
  • Common garden
  • Snake venom
  • Sistrurus
  • Toxicity