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Fatty acid and stable isotope characteristics of sea ice and pelagic particulate organic matter in the Bering Sea: tools for estimating sea ice algal contribution to Arctic food web production

Abstract

We determined fatty acid (FA) profiles and carbon stable isotopic composition of individual FAs (δ13CFA values) from sea ice particulate organic matter (i-POM) and pelagic POM (p-POM) in the Bering Sea during maximum ice extent, ice melt, and ice-free conditions in 2010. Based on FA biomarkers, differences in relative composition of diatoms, dinoflagellates, and bacteria were inferred for i-POM versus p-POM and for seasonal succession stages in p-POM. Proportions of diatom markers were higher in i-POM (16:4n-1, 6.6–8.7 %; 20:5n-3, 19.6–25.9 %) than in p-POM (16:4n-1, 1.2–4.0 %; 20:5n-3, 5.5–14.0 %). The dinoflagellate marker 22:6n-3/20:5n-3 was highest in p-POM. Bacterial FA concentration was higher in the bottom 1 cm of sea ice (14–245 μg L−1) than in the water column (0.6–1.7 μg L−1). Many i-POM δ13CFA values were higher (up to ~10 ‰) than those of p-POM, and i-POM δ13CFA values increased with day length. The higher i-POM δ13CFA values are most likely related to the reduced dissolved inorganic carbon (DIC) availability within the semi-closed sea ice brine channel system. Based on a modified Rayleigh equation, the fraction of sea ice DIC fixed in i-POM ranged from 12 to 73 %, implying that carbon was not limiting for primary productivity in the sympagic habitat. These differences in FA composition and δ13CFA values between i-POM and p-POM will aid efforts to track the proportional contribution of sea ice algal carbon to higher trophic levels in the Bering Sea and likely other Arctic seas.

Introduction

The contribution of ice algae to total primary production in the seasonally ice-covered Bering Sea is estimated to be between 3 and 30 % (McRoy and Goering 1976; R. R. Gradinger, unpublished data). Thus, the quantitative importance of sea ice algae to marine food webs in Arctic seas such as the Bering Sea remains uncertain (Hobson et al. 1995; Søreide et al. 2006; Budge et al. 2008). In the Bering Sea, the timing of sea ice melt determines the timing of spring phytoplankton blooms, the sources of spring primary production (sympagic or pelagic), and the fate of carbon from this primary production to the marine food web (Walsh and McRoy 1986; Hunt et al. 2002, 2011; Hunt and Stabeno 2002). Climate-related changes in the timing of seasonal sea ice cover affect spring primary production patterns (Stabeno et al. 2001, 2010; Hunt and Stabeno 2002), and these changes will likely propagate through the food web to upper trophic levels (Grebmeier et al. 2006; Bluhm and Gradinger 2008; Leu et al. 2011; Grebmeier 2012). Furthermore, climate-induced changes in the food quality, such as fatty acid (FA) content derived from sympagic and pelagic primary production, could ultimately influence upper trophic levels (Litzow et al. 2006; Leu et al. 2011).

FA profiles and biomarkers of sea ice algae and phytoplankton have been used to understand the feeding ecology of consumers in a wide range of Arctic seas (e.g., Lewis 1969; Falk-Petersen et al. 1998; Auel et al. 2002; Peters et al. 2004; Budge et al. 2007; Mayzaud et al. 2013). Changes in phytoplankton species composition, productivity, physiological state, and amount of living versus decayed material within the marine particulate organic matter (POM) pool can be inferred from variations in FA profiles of POM (Mayzaud et al. 1989; Reuss and Poulsen 2002; Mayzaud et al. 2013). In addition to FA biomarkers, bulk carbon stable isotopic analyses of organisms have been used to examine the feeding ecology of Arctic marine organisms and food webs (e.g., Dehn et al. 2007; Iken et al. 2010; Feder et al. 2011; Weems et al. 2012). In response to increased seasonal light availability (Gradinger et al. 2009) and a carbon-limiting environment of the closed sea ice system (e.g., Fischer 1991; Schubert and Calvert 2001; Kennedy et al. 2002), POM in sea ice (i-POM) has carbon stable isotope values (expressed here as δ13C values) that are more enriched in 13C than pelagic POM (p-POM) (Hobson et al. 1995; Naidu et al. 2000; Schubert and Calvert 2001; Gradinger et al. 2009; Thomas et al. 2010). In a combination of FA and stable isotope analysis methods, carbon stable isotope analysis of specific FAs (δ13CFA values, e.g., 16:4n-1 and 20:5n-3) in sea ice algae and pelagic phytoplankton have been investigated (Budge et al. 2008). Sea ice algae δ13CFA values were higher than pelagic phytoplankton, which allowed for the quantitative estimation of the proportional contribution of sympagic and pelagic primary production to consumers in the Arctic (Budge et al. 2008).

The δ13CFA data from sea ice algae and marine phytoplankton so far have been restricted to the Chukchi Sea region off of Barrow, Alaska (Budge et al. 2008). Furthermore, the temporal and spatial variability of δ13CFA values from marine algae from high latitudes, and the processes that generate this isotopic variability, are poorly resolved. Elucidating these patterns and processes in the Bering Sea is important to help interpret δ13CFA values at higher trophic levels as a way of estimating the importance of sea ice algal carbon and pelagic carbon to food web production. Our goals were to characterize the FA profiles and the δ13CFA values of i-POM and p-POM in the Bering Sea, examine seasonal differences, and determine if there was a correlation between δ13CFA values and day length (used here as a proxy for light availability). We hypothesize that differences in FA profiles between POM types can be explained by variation in algal taxon composition. In addition, greater seasonal availability of light at the most southerly sea ice locations in the Bering Sea should stimulate the highest levels of sea ice primary production (McRoy and Goering 1974). This high primary production within the sea ice habitat would then lead to a subsequent 13C enrichment of individual FAs as a result of decreased isotopic discrimination during photosynthesis in the carbon-limited and closed sympagic habitats.

Materials and methods

Sample collection

i-POM and p-POM samples were collected as part of the Bering Sea Ecosystem Study/Bering Sea Integrated Ecosystem Research Program during three major seasonal ice regimes in 2010 based on timing of sea ice conditions in the northern and central portions of the Bering Sea: maximum ice extent (13-28 March; i-POM n = 12, p-POM n = 14), ice melt (11 May-10 June; i-POM n = 1, p-POM n = 20), and ice-free conditions (18 June–10 July; p-POM n = 15) (Fig. 1). One p-POM sample was taken from a station in the deep basin of the Bering Sea which did not experience ice cover (Fig. 1). Maximum ice extent occurred on 31 March 2010 and all stations on the Bering Sea shelf experienced ice cover between 21 March and 10 April 2010 (Cavalieri et al. 1996; Richter-Menge and Overland 2010). For the determination of i-POM in March 2010, sea ice cores were collected with an ice corer (9 cm inner diameter) and cut into sections of 1–10 cm thickness (Cooper et al. 2013). Data in this study came from samples taken from the bottom 1-cm sections of the sea ice cores, which were completely melted in the dark and filtered on pre-combusted GF/F filters (melted water volumes ranged from 1.8 to 18.5 mL). The i-POM sample taken during ice melt consisted mainly of Melosira arctica (K. Iken, personal observation) collected from overturned ice floes. During maximum ice extent p-POM samples were collected at 15 m below the surface. During ice melt and ice-free conditions, samples were collected at the chlorophyll maximum layer between a depth of 5–29 m from the surface. Each p-POM sample was collected from a single Niskin bottle and sample volumes were between 200 and 1,500 mL. The algal composition in p-POM samples taken during ice melt was described for some stations and contained Thalassiosira sp., Nitzschia frigida, Fragillariopsis sp., Chaetoceros sp., Pseudonitzschia sp., and Phaeocystis sp. (K. Iken, personal observation). Samples were filtered using a GF/F filter (pore size 0.7 μm) and stored in a vial of chloroform at −20 °C until laboratory analysis.

Fig. 1
figure1

Sampling stations for sea ice particulate organic matter (i-POM; open symbols) and pelagic POM (p-POM; shaded symbols) collected in the Bering Sea in 2010 during maximum ice extent 13–28 March (circles; i-POM n = 12, p-POM n = 14), ice melt between 11 May–10 June (squares; i-POM n = 1, p-POM n = 20), and ice-free (triangles; p-POM n = 15) conditions between 18 June and 10 July 2010. Ice conditions were based on the timing of sea ice conditions in the northern and central portions of the overall sampling area

Details for the determination of ice bulk properties are described by Cooper et al. (2013). In short, filters were dried, acid fumed, and analyzed at the Alaska Stable Isotope Facility (ASIF) at the University of Alaska Fairbanks (UAF) for determination of bulk i-POM particulate organic carbon (POC) concentrations and δ13C values (Cooper et al. 2013). A second set of filters was analyzed for chlorophyll a concentrations using fluorometric determination after extraction in 90 % acetone.

FA analysis

Lipids were quantitatively extracted from all samples using 2:1 chloroform/methanol at 20–30 parts solvent to sample (Folch et al. 1957; Parrish 1999). FA methyl esters (FAMEs) were prepared using an acidic transesterification (Budge et al. 2006). FAMEs were quantified using temperature-programmed gas chromatography (GC) on a Perkin Elmer Autosystem II Capillary FID gas chromatograph fitted with a 30-m-long × 0.25-mm-internal diameter column coated with 50 % cyanopropyl-methylpolysiloxane (DB-23) and linked to a computerized integration system (Varian Star software). Shorthand nomenclature of A:Bn-X was used to describe each FAME, where A represents the number of carbon atoms, B the number of double bonds and X the position of the double bond closest to the terminal methyl group. Approximately 70 FAMEs were identified by comparison of retention times with known standards (Nu Check Prep, Elysian, MN), or using GC-mass spectrometry. Concentrations of each FA were determined from a standard (α-cholestane; Sigma-Aldrich) with a known mass and volume of the sample filtered.

Compound-specific stable isotope analysis of individual FAs

δ13C of samples (expressed in per mil—‰) were analyzed from FAME sub-samples by routing the effluent from a GC (Trace GC Ultra; Thermo, Bremen, Germany) through a combustion interface (Finnigan GC combustion III; Thermo) to an isotope ratio mass spectrometer (IRMS) (Thermo Finnigan Delta V; Thermo) at ASIF, UAF. The same GC column and method described above for FID analyses of FAMEs were used to separate the FAMEs for analysis using IRMS. To correct for any influence of carbon from the methyl group added during transesterification and determine any kinetic isotope effects, free FA (FFA) standards 16:0 and 18:0 were transesterified with the same reagents described above. The purity of the FFA was confirmed with thin layer chromatography prior to transesterification and δ13C values for FFA were determined using an elemental analyzer (Costech ECS4010) routed to an IRMS. The δ13C values of the respective FAMEs of these FFA that had been transesterified into their respective FAMEs were then also measured using the GC-IRMS system described above. We could not perform this analysis on all the FA examined because most are not commercially available. There was no evidence of a kinetic isotope effect associated with transesterification, which is expected for a reaction that goes to completion (Rieley 1994). The difference between the δ13C values for FFA and FAME with the same chain length was entirely due to the added methyl group. The proportional contribution of this methyl group to a given FA depends on the chain length. Therefore, an average δ13C value for this added methyl group was calculated using the following equation:

$$\delta^{13} {\text{C}} = (n + 1)[\delta^{13} {\text{C}}_{\text{FAME}} ] - n[\delta^{13} {\text{C}}_{\text{FFA}} ],$$

where n is the number of carbon atoms in the FFA (Abrajano et al. 1994). We then calculated an average δ13C value (−48.8 ± 1.3 ‰, mean ± 1 SD) for the methyl-derived carbon based on the difference between the δ13C values of the corresponding FFA and their respective FAME. This value was based on three runs each of the C16 and C18 standards, and used to correct our FAME data by applying the above equation. The δ13C values from the FAMEs were also calibrated using a standard mixture consisting of ethyl and methyl esters of 14:0, 16:0, 18:0, and 20:0 (supplied by Indiana University Stable Isotope Reference Materials) where the coefficient of determination (r 2) of the measured versus expected relationship was >0.99. A C16 laboratory standard was analyzed after every ten samples to track analytical error of the GC-IRMS system, which was ≤0.8 ‰ (representing the 1 SD of 14 analyses of the C16 standard interspersed during the samples runs). All δ13C values are reported relative to Vienna Pee Dee Belemnite using standard notation, where δ13C (‰) = [(R sample/R standard) − 1] × 1,000, and R is the corresponding ratio of 13C/12C.

Data analysis

Bray–Curtis similarity matrices and permutational multivariate ANOVA (PERMANOVA; Anderson 2001; McArdle and Anderson 2001) were used to investigate the variation in FA composition, based on 59 FAs present in proportions >0.1 %, between i-POM and p-POM, and seasonal variation within p-POM using PRIMER version 6 (Primer-E). Non-metric multidimensional scaling plots were used to visualize differences among FA profiles of samples. A stress value of <0.1 indicates good fit of the data with little prospect of incorrect interpretation (Clarke 1993). Similarity percentages routines (SIMPER) were performed to determine FAs contributing most to the observed differences. Data were standardized to 100 % and log(1 + x) transformed prior to analysis.

We examined the proportion of 16:4n-1 and the ratios of 16:1/16:0 and 22:6n-3/20:5n-3 to assess the presence and dominance of diatoms and dinoflagellates in the POM samples (Claustre et al. 1988, 1989; Budge and Parrish 1998). Additionally, the sum of 15:0, 17:0, and the iso- and anteiso-FAs (i-14:0, i-15:0, ai-15:0, i-16:0, i-17:0, and ai-17:0) were used to determine the contribution of bacterial FAs in the POM samples (Budge and Parrish 1998).

δ13CFA values were determined for 39 FAs. Not all FAs were present in sufficient quantities to be analyzed by GC-IRMS. Out of these 39 FAs, 13 (14:0, 16:0, 16:1n-11/9, 16:1n-7, 16:2n-4, 16:3n-4, 16:4n-1, 18:0, 18:1n-9, 18:2n-6, 18:4n-3, 20:5n-3, and 22:6n-3) were selected for further data analysis because they were identified in most of the samples (63–100 %). FAs 16:1n-11 and 16:1n-9 co-eluted on the GC-IRMS and are referred to here as 16:1n-11/9. Data were not normally distributed, therefore a non-parametric Mann–Whitney U-test was used to assess differences in δ13CFA values of the selected 13 FAs between i-POM and p-POM during maximum ice extent. A Kruskal–Wallis ANOVA was used followed by Bonferroni adjustment for multiple comparisons to test for differences in δ13CFA values of each FA in p-POM during maximum ice extent, ice melt, and ice-free conditions. Spearman’s rank order correlations were performed to examine any correlations between δ13CFA values of the 13 selected FAs in both i-POM and p-POM samples and day length. A site-specific estimate of light exposure for each station was determined using the U.S. Naval Observatory website http://aa.usno.navy.mil/data/docs/RS_OneDay.php. Significance level α was controlled for multiple comparisons with Holm’s sequential Bonferroni adjustments. Spearman’s rank order correlations were also performed between day length and POM FA concentrations to determine if FA production increased with light exposure (day length). In addition, correlations were examined between day length, POC concentrations, chlorophyll a concentrations, and bulk δ13C values measured in i-POM to determine if productivity increased with day length within the closed sea ice system.

We estimated the fraction of source ocean water dissolved inorganic carbon (DIC) used for FA synthesis in sea ice during maximum ice extent using the following Rayleigh equation modified for isotope reaction progress in a closed system (Fry 2006):

$$\delta_{\text{AP}} = \delta_{\text{Source}} + \varDelta *((1 - f)/f)*\ln (1 - f),$$

where, for each FA, the δ13C value of the accumulated product (δAP) is the δ13CFA value measured from i-POM, δSource is the δ13C value of ocean water DIC, Δ is the difference between the δ13C value of the δSource and instantaneous product (the δ13C value of product at any instant in time), and f is the fraction or percentage of source (i.e., DIC) reacted. We used two δ13C values for DIC from previous studies: the mean for surface water of the Pacific Ocean (1.55 ‰) determined by Quay et al. (2003), and the value at the most northerly station in the Pacific Ocean (1.85 ‰) reported by Gruber et al. (1999). p-POM was measured in an open system during the same time and did not vary with day length (see “Results”). Thus, we assumed that the average δ13CFA value from p-POM during maximum ice extent for each 13 FA could be used to approximate the i-POM δ13CFA value when the fraction of the source reacted was zero (i.e., p-POM represents i-POM before isotopic discrimination results from the closed sea ice conditions). These values were used to calculate Δ for each FA (Δ = average δ13CFA of p-POM − δSource). The fraction reacted for each FA at a given day length was determined using the Solver Function in Microsoft Excel (version 14.3.2 for Mac 2011). Linear regression was performed between day length and the fraction reacted for the 13 selected FAs to determine if day length could be used to predict the fraction of source reacted. The Mann–Whitney U-test, Kruskal–Wallis ANOVA, Spearman’s rank order correlations and linear regression were performed using Statistica version 12 (StatSoft).

Results

FA content

The FA profile from the sample from the deep basin was different than that of other samples collected over the Bering Sea shelf during ice melt (Fig. 2), and because the station from the deep basin did not experience ice cover it was not included in further data analyses. FA profiles were significantly different between i-POM and p-POM, and also varied seasonally within p-POM (Fig. 2; PERMANOVA, P = 0.001). Average dissimilarity between i-POM and p-POM FA profiles during maximum ice extent was 31.8 % (SIMPER) with the diatom FAs 16:4n-1 and 20:5n-3 contributing most to the dissimilarity (7.1 and 5.9 %, respectively). These two FAs were present in greater proportions in i-POM (16:4n-1, 6.6–8.7 %; 20:5n-3, 19.6–25.9 %) than in p-POM (16:4n-1, 1.2–4.0 %; 20:5n-3, 5.5–14.0 %). i-POM samples collected during maximum ice extent had lower proportions of 14:0, 16:0, 16:1n-7 and 20:5n-3, and higher proportions of 18:0, 16:1n-11, 18:1n-9, 18:1n-7, 20:1n-7, 16:4n-1, 18:4n-3 and 22:6n-3 compared to the M. arctica sample collected during ice melt (Fig. 3). Within p-POM, FA profiles were most dissimilar between maximum ice and ice melt conditions (33.7 %, SIMPER). Dissimilarity during maximum ice and ice-free conditions, and ice melt and ice-free conditions was 27.1 and 23.0 %, respectively. FA profiles of p-POM also showed some seasonal patterns with proportions of 16:1n-9, 18:0, 18:1n-9, 20:1n-7 and 22:1n-9 being relatively higher during maximum ice extent, lower during ice melt, and higher again during ice-free conditions. Proportions of 22:6n-3 in p-POM increased seasonally (Fig. 3). Total amounts of saturated FA (SAT), monounsaturated FA (MUFA), and polyunsaturated FA (PUFA) varied by POM type and seasonally within p-POM (Fig. 3). The amount of SAT from i-POM (22.0–26.3 %) was lower than that from p-POM (26.8–34.9 %). During maximum ice extent and ice melt conditions, levels of MUFA from i-POM were also lower (31.3–32.0 %) than from p-POM (40.6–42.3 %). In contrast, the amount of PUFA from i-POM (41.9–43.8 %) was higher than that from p-POM (18.6–34.8 %).

Fig. 2
figure2

Non-metric multidimensional scaling (MDS) plot of i-POM (open symbols) and p-POM (shaded symbols) using 59 fatty acids (FAs) present in proportions >0.1 % in samples collected in 2010 during maximum ice extent (circles; i-POM n = 12, p-POM n = 14), ice melt (squares; i-POM n = 1, p-POM n = 20), and ice-free (triangles; p-POM n = 15) conditions from all stations; data from the deep basin station are circled (two-dimensional stress = 0.09). For other abbreviations, see Fig. 1

Fig. 3
figure3

Proportions of some FAs (mean ± SE) in i-POM (open symbols) and p-POM (shaded symbols) collected in 2010 during maximum ice extent (circles; i-POM n = 12, p-POM n = 14), ice melt (squares; i-POM n = 1, p-POM n = 20), and ice-free (triangles; p-POM n = 15) conditions. For abbreviations, see Figs. 1 and 2

Diatom biomarkers (16:1/16:0, 16:4n-1) were higher than the dinoflagellate biomarker (22:6n-3/20:5n-3) in i-POM (Fig. 4a). This was also observed in p-POM samples during ice melt. However, in p-POM the levels of 16:4n-1 were lower than in i-POM and the ratios of 16:1/16:0 and 22:6n-3/20:5n-3 were similar in p-POM samples during maximum ice extent and ice-free conditions (Fig. 4a). The bacterial indicator (i.e., the sum of 15:0, 17:0, and the iso- and anteiso FA) was <4 % of the total FAs in all POM samples (Fig. 4b). However, the concentration of the bacterial indicator in the bottom 1 cm of sea ice ranged from 14 to 245 μg L−1, which was on average 115 times greater than the bacterial indicator in the water column during maximum ice extent. The concentrations of the bacterial indicator in the water column during maximum ice extent, ice melt, and ice-free conditions were 0.6 ± 0.3, 1.7 ± 0.8, 0.6 ± 0.2 μg L−1 (mean ± SD), respectively.

Fig. 4
figure4

a Diatom FA indicators 16:1/16:0, 16:4n-1, and dinoflagellate indicator 22:6n-3/20:5n-3 and b bacterial FA indicator (sum of 15:0, 17:0 and all iso- and anteiso-branched chain FAs) in i-POM and p-POM collected during maximum ice extent (Max; i-POM n = 12, p-POM n = 14), ice melt (Melt; i-POM n = 1, p-POM n = 20), and ice-free (Free; p-POM n = 15) conditions (mean ± SE). For other abbreviations, see Figs. 1 and 2

In March 2010, total concentrations of FAs in the bottom 1 cm of sea ice ranged from 721 to 28,000 μg L−1, which was 60 to almost 1,000 times greater than concentrations measured in the water column (8–542 μg L−1). FA concentrations in the water column showed seasonal change with the highest concentrations measured during ice melt in all FA except for 18:1n-9 (Fig. 5). Concentrations of n-3 and n-6 PUFA in the bottom 1 cm of sea ice ranged from 123 to 9,500 and 42–732 μg L−1, respectively. These values were also higher than those found in the water column (n-3 PUFA, 2.9–29.6 μg L−1; n-6 PUFA, 0.8–2.3 μg L−1). FA concentrations in the bottom 1 cm of sea ice increased with day length for all FAs (R 2 > 0.48), except 18:1n-9 (R 2 = 0.16, Spearman’s P = 0.60). FA concentrations also increased with increasing POC and chlorophyll a concentrations measured in the bottom 1 cm of sea ice (R 2 > 0.48).

Fig. 5
figure5

Boxplot of concentrations of 13 FAs in the water column. For each FA, the first boxplot represents maximum ice extent (n = 14), the second ice melt (n = 20), and the third ice-free conditions (n = 15). Ends of the boxes define the 25th and 75th percentiles, the line is at the median, the whiskers define the 10th and 90th percentiles, and the circles represent outliers. Note log scale used on y-axis

δ13CFA values

The δ13CFA values of 13 selected FA varied between POM types and among seasonal p-POM samples (Fig. 6). During maximum ice extent, δ13CFA values from i-POM were higher compared with those from p-POM (Mann–Whitney U-test, P < 0.05; difference between means ranging from 1.2 to 7.9 ‰), with the exception of 16:2n-4, 16:3n-4, 16:4n-1, and 18:1n-9. Within p-POM samples, δ13CFA values were significantly different among ice regimes for 18:0 and the unsaturated FAs 16:3n-4, 18:1n-9, 18:2n-6, and 18:4n-3 (Kruskal–Wallis ANOVA, P < 0.04; Fig. 6). The δ13CFA values from i-POM increased with increasing day length for all FAs measured (Fig. 7a). Correlations between δ13CFA values of i-POM and day length were significant (R 2 > 0.73, Spearman’s P < 0.01) for all FAs except for 18:1n-9 (R 2 = 0.50, Spearman’s P = 0.60). We found no correlation between δ13CFA values of the 13 selected FAs in p-POM and day length during maximum ice extent and ice melt conditions (Spearman’s P > 0.05). Only the δ13CFA value of 22:6n-3 (R 2 = −0.73, Spearman’s P = 0.003) in p-POM decreased significantly with day length during ice-free conditions.

Fig. 6
figure6

δ13CFA values for 13 select FAs (mean ± SE) in i-POM (open symbols) and p-POM (shaded symbols) samples during maximum ice extent (circles; i-POM n = 10–12, p-POM n = 3–12), ice melt (squares; i-POM n = 1, p-POM n = 14–20), and ice-free (triangles; p-POM n = 1–14) conditions in 2010. Sample sizes are given as ranges because not all FAs were present in sufficient quantities to be analyzed by gas chromatography–isotope ratio mass spectrometry in each sample. These 13 FAs were identified in the majority of the samples (63–100 %). Different letters indicate differences between values within p-POM (Kruskal–Wallis ANOVA, P < 0.04). VPDB Vienna Pee Dee Belemnite; for other abbreviations, see Figs. 1 and 2

Fig. 7
figure7

a Day length and δ13CFA values for FA 16:1n-7 in i-POM (open symbols, n = 12) and p-POM (shaded symbols, n = 12) during maximum ice extent conditions in 2010. Spearman’s rank correlations for all other i-POM FAs and day length were significant except for 18:1n-9; b isotopic fractionation (Δ) in a closed system. Δ is the difference between the residual substrate (RS) and instantaneous product (IP). In a closed system such as sea ice habitat, the source is ocean dissolved inorganic carbon [shaded horizontal line at 1.85 ‰ (Gruber et al. 1999)] and the accumulated product (AP) is measured i-POM. For other abbreviations, see Figs. 1, 2 and 6

POC concentration and bulk δ13C values measured in i-POM during maximum ice extent significantly increased with day length (Spearman’s R 2 > 0.66, P < 0.03). In p-POM, only the concentration of 22:6n-3 significantly increased with day length (Spearman’s R 2 = 0.45, P = 0.002). The average fractionation (Δ) between ocean water DIC and i-POM during maximum ice extent for the 13 selected FAs ranged from 26.6 to 33.9 ‰ and the percentage of DIC assimilated into FA synthesis at the longest day ranged from 12 to 73 % (Fig. 8). Day length and fraction reacted were significantly positively correlated for all FAs (P < 0.02), except for 18:1n-9 (Spearman’s R 2 = 0.41, Fig. 8).

Fig. 8
figure8

Day length and fraction of source dissolved inorganic carbon (DIC) reacted for 12 FAs of i-POM during maximum ice extent conditions in 2010. For all FA n = 12, except for 16:1n-11/9 (n = 11), 16:3n-4 (n = 10), and 18:0 (n = 11). Results shown for a source DIC value of 1.85 ‰ (Gruber et al. 1999). Results from using a value of 1.55 ‰ from Quay et al. (2003) gave similar results that could not be distinguished in the figure. Linear regressions were significant for all FA shown (P < 0.02). Data for 18:1n-9 not shown because they were not significant. For other abbreviations, see Figs. 1 and 2

Discussion

FA content

The FA biomarkers indicated that the relative amounts of diatoms, dinoflagellates, and bacteria in i-POM differed from those in p-POM and changed seasonally within p-POM. Although algal species were not identified in these samples, the algal class composition of POM can be partially inferred using FA biomarkers (reviewed in Dalsgaard et al. 2003). Using 16:4n-1 and the ratio of 16:1/16:0 as diatom biomarkers and the ratio of 22:6n-3/20:5n-3 as a dinoflagellate biomarker, i-POM during maximum ice extent appears to be dominated by diatoms, as is typical for Arctic sympagic communities (Horner 1985; Gradinger 2002; Arrigo et al. 2010). The ratios of the diatom marker 16:1/16:0 in the i-POM sampled during maximum ice extent, the M. arctica sampled during ice melt, and also the p-POM sampled during ice melt had similar values to those found in ice algae (2.7) and phytoplankton (2.3) from the Chukchi region (Budge et al. 2008). Additionally, the FA composition of the M. arctica sampled during ice melt confirmed that these FA ratios are a good diatom indicator in Arctic sea ice. In comparison, the ice algae sample from Budge et al. (2008), which was dominated by the pennate diatom Navicula sp., contained almost twice as much of the diatom marker 16:1n-7 (50.0 %) and approximately three times less of diatom markers 16:4n-1 (2.1 %) and 20:5n-3 (9.6 %) than our centric diatom M. arctica sample. Proportions of other FAs were similar between our M. arctica sample and the Navicula sp. samples from Budge et al. (2008). Different diatom species can produce different proportions of FA (i.e., Viso and Marty 1993, Dunstan et al. 1994). Thus, species differences may explain the striking differences in the three diatom markers (16:1n-7, 16:4n-1, and 20:5n-3) in the proportions of some FAs between our M. arctica sample and the Navicula sp. samples from Budge et al. (2008). Differences in environmental conditions between the Bering and Chukchi Seas at the time of sampling may have also contributed to the differences in FA profiles between these two diatom species as temperature, nutrient, and light availability are known to influence phytoplankton FA composition (Harrison et al. 1990; Thompson et al. 1992; Leu et al. 2010).

The relative abundance of diatom marker 16:4n-1 from p-POM taken during maximum ice extent was almost an order of a magnitude lower than that of i-POM, which could indicate lower diatom abundance in p-POM under heavy ice conditions or that concentrations of diatom FAs in p-POM were lower than in i-POM. The relative amounts of diatom markers 16:1/16:0 and 16:4n-1 for p-POM doubled in amount during ice melt compared with those measured during maximum ice extent. The presence of diatoms in p-POM during ice melt was confirmed by the observation of diatoms such as Thalassiosira sp., N. frigida, Fragilariopsis sp., Chaetoceros sp., and Pseudo-nitzschia sp., in these p-POM samples, with only three of 21 stations having notable amounts of the prymnesiophyte Phaeocystis (K. Iken, personal observation). Furthermore, the relative proportions of 16:0, 18:0, 16:1n-7, 18:1n-9, 18:1n-7, 20:1n-11, 20:1n-9, 20:1n-7, 16:4n-1, 20:5n-3, and 22:6n-3 in p-POM during ice melt are very similar to those found in the pelagic phytoplankton samples from Budge et al. (2008), which comprised mainly of the large centric diatom Coscinodiscus sp. This similarity also suggests that the p-POM samples during ice melt contained diatoms. The diatom species present during maximum ice extent are unknown but the diatom marker FAs during that time was much lower than during ice melt. The relative amounts of diatom markers 16:1/16:0 and 16:4n-1 for p-POM were lowest during maximum ice extent and ice-free conditions, and highest during ice melt while the dinoflagellate indicator 22:6n-3/20:5n-3 showed the opposite pattern. This seasonal change in dominance of diatoms is consistent with a succession of taxa from low levels of primary production during maximum ice extent limited by light and stratification, transitioning to a water column seeded by diatoms released from sea ice as it melted during a spring bloom (McRoy and Goering 1974; Alexander and Niebauer 1981; Jin et al. 2007). When the ice retreats in the Bering Sea, the diatom bloom community can be succeeded by non-diatom phytoplankton such as dinoflagellates, haptophytes such as Phaeocystis sp., and Synechococcus sp., and cryptophytes (Moran et al. 2012). Although 22:6n-3/20:5n-3 has been historically used as a dinoflagellate indicator because 22:6n-3 can be produced in large amounts by dinoflagellates, 22:6n-3 can also be biosynthesized by heterotrophic flagellates (e.g., Desvilettes and Bec 2009, Bec et al. 2010). Therefore, it is possible that an increase in 22:6n-3/20:5n-3 of p-POM from ice melt to ice-free conditions may be due to a shift toward a stronger microbial food web with heterotrophic protists. The increased contribution of bacteria to p-POM is further supported by the increase in the bacterial biomarkers in the water column during ice melt to ice-free conditions (see below). Most likely the increase in 22:6n-3/20:5n-3 reflects a combination of increased dinoflagellate production with concurrent decreases in diatom production, as well as contributions from heterotrophic protists that can synthesize 22:6n-3 from other FA precursors such as 20:5n-3.

The proportion of bacterial FA markers indicates the presence of bacteria in POM, and the concentration of this marker suggests a greater total abundance of bacteria in the bottom 1 cm of sea ice than in the water column. In fact, bacteria can contribute up to 50 % of the total biomass within sea ice (Gradinger and Zhang 1997) and can be up to 100 times greater in abundance in Arctic sea ice relative to the water column (Maranger et al. 1994). In comparison, the bacterial FA marker in ice algae from the Chukchi region (1.4 %) was lower than that found in i-POM during maximum ice extent and higher than the bacterial marker in M. arctica. The bacterial FA marker in pelagic phytoplankton from the Chukchi region (0.9 %) was lower than those in p-POM (1.8–3.6 %), indicating a higher relative contribution of bacteria in p-POM. In addition to algae and bacteria, the POM samples most likely contained small amounts of detritus and microzooplankton that may have influenced FA profiles of these samples. Within sea ice, meiofauna typically contribute a minor fraction to total biomass and heterotrophic flagellates can be an important source of carbon (Gradinger et al. 1999). However, the FA profiles of i-POM and p-POM (during ice melt) were similar to those found in pure ice algae and pelagic phytoplankton from the Chukchi Sea (Budge et al. 2008); therefore, the presence of material other than algae is likely negligible.

In addition to the differences and seasonal changes in community composition of POM in the Bering Sea, the nutritional quality (i.e., levels of PUFA) of POM within sea ice and the water column also varied, as well as seasonally within the water column. Seasonal increase of FA concentrations within the water column during ice melt could be explained by an increase of phytoplankton cells during the spring bloom or from ice algae being released into the water column. The diatom FA biomarkers 16:1/16:0 and 16:4n-1 suggest that the increases in relative proportions of FAs in p-POM during ice melt are largely from the release of i-POM. However, if p-POM during ice melt was largely influenced by sinking i-POM we might expect the δ13CFA of p-POM to be similar to that of i-POM, but this is not the case. It is likely that the p-POM during ice melt is influenced by both the sinking of i-POM and active p-POM production in the water column. The concentrations of n-3 and n-6 PUFA in the bottom 1 cm of sea ice ranged from 40 to almost 300 times greater than those measured in the water column. The higher PUFA levels in sea ice compared to the water column indicated that the material in the bottom 1 cm of sea ice has a higher, concentrated, nutritional value than that of material in the water column, likely making the sea ice habitat an important, concentrated source of PUFA for grazers (e.g., Falk-Petersen et al. 1998) and presumably for higher trophic levels (e.g., Bluhm et al. 2010). For example, the Arctic bivalve Macoma balthica preferentially consumed sea ice algae over phytoplankton, and both M. balthica and the amphipod Monoporeia affinis had higher levels of PUFA and total FAs when fed on sea ice algae compared to phytoplankton (Sun et al. 2009). Thus, these two Arctic benthic species may provide an important source of PUFA for animals that consume them. A similar observation was noted in the copepod Calanus glacialis in Rijpfjorden, Svalbard, Norway where its FA composition indicated that sea ice algae were an important food source during the spring bloom, when ice algae, not pelagic algae, triggered the reproduction of zooplankton (Leu et al. 2011). It may be that sea ice algae are preferentially eaten by some consumer taxa simply because they are present in larger concentrations than phytoplankton when released from the sea ice (e.g., Carroll and Carroll 2003). However, higher ice algal concentrations would also provide greater concentrations of PUFA to consumers.

δ13CFA values

In addition to differences in FA profiles between i-POM and p-POM, and variances in the nutritional content between sea ice and the water column, our data suggest that there are fundamental differences in the processes influencing FA production in the sympagic versus pelagic habitat. Overall, most of the 13 FAs from i-POM analyzed for their δ13C were more enriched in 13C compared to p-POM, including the diatom biomarkers 16:4n-1 and 20:5n-3. These results agree with some previous bulk and compound-specific carbon stable isotope analyses of sea ice algae and i-POM that have shown them to be more enriched in 13C than pelagic phytoplankton and p-POM (Hobson et al. 1995; Gibson et al. 1999; Søreide et al. 2006, 2013; Budge et al. 2008; Tamelander et al. 2008). This implies that isotopic reaction processes during FA production differ markedly between the sympagic and pelagic environments in which the FAs were synthesized. The i-POM δ13CFA values increased with increasing day length, while the δ13CFA values from p-POM generally showed relatively little variation with increasing light availability during the same sampling period. We suggest that these patterns are consistent with a substantial influence of the photosynthetic isotope effect on FA production of i-POM from a limited DIC substrate within the semi-closed/closed sympagic system (Kennedy et al. 2002; Papadimitriou et al. 2007). These relatively higher photosynthetic demands within sea ice force decreased 13C discrimination during photosynthesis and the subsequent increase in δ13CFA values in sympagic FAs, while pelagic production can draw from a constantly renewed DIC pool.

Patterns observed in δ13CFA values with increasing day length in the two POM types during maximum ice extent allow reaction progress associated with FA synthesis in sea ice to be inferred. Firstly, the δ13CFA values for many of the FAs of i-POM and p-POM were similar at the lowest day length, which is an indication of the baseline fractionation associated with FA synthesis relative to source DIC. Secondly, the δ13CFA values of p-POM FAs were relatively invariable over the light availability gradient, which is consistent with FA synthesis by primary production in an open system where source DIC is constantly being replenished. Thirdly, bulk δ13C values measured in i-POM increased, as did the δ13CFA values for many of the sympagic FAs, with increased light availability, which is consistent with a reaction progress in a closed system (e.g., Fry 2006). Finally, the δ13CFA values of many of the sympagic FAs differed under the highest light conditions relative to typical marine DIC values, which implies that the fraction of the source reacted increased with increasing day length, but reaction progress did not go to completion. In other words, when the reaction progress is complete, the δ13CFA value of i-POM should equal that of the initial δ13C value of source DIC (Fry 2006). The estimated 12–73 % of source DIC reacted indicated that in the semi-closed/closed sea ice system, DIC was not completely limiting and was still available for FA synthesis.

There are many factors that, in combination, contribute to the variability in our estimates of reaction progress among sympagic FAs. One source of variability could be that synthesis pathways may be different for the various FAs analyzed. On average, SAT and PUFA gave similar estimates of reaction progress (51 and 50 %, respectively) while, overall, MUFA gave a higher estimate of reaction progress (64 %). This may reflect the activities of different desaturase enzymes used to insert bonds versus elongation enzymes used to add carbon to FAs. Another source of variability in reaction progress estimates could stem from the δ13C values for surface water DIC. The δ13C values for surface water DIC in the Bering Sea are not available, therefore, we used values estimated for the entire Pacific Ocean and also from the Gulf of Alaska (Gruber et al. 1999; Quay et al. 2003), which may not reflect the true value for our study area. However, the range of source DIC δ13C values we used provided very similar estimates of fraction of source DIC reacted. We suggest that additional factors besides day length may also influence reaction progress estimates for sympagic FA synthesis (i.e., nutrient availability, brine convection, internal carbon mineralization). An incomplete reaction progress could also be due to sampling time, i.e., day length at the time of sampling may not have been great enough. Replenishment of the DIC pool could also lead to an incomplete reaction progress. This could also occur from the recycling of carbon from the breakdown of organic matter within sea ice and if the sympagic system was a semi- rather than a fully closed system. The most parsimonious explanation is that ice breakup occurs before the reaction of DIC can go to completion, and there is insufficient time to exhaust the DIC pool within sea ice. Further research is needed to determine if δ13CFA values of POM vary regionally and annually, and what factors may influence reaction progress estimates for sympagic FA synthesis.

Conclusion

In summary, we found differences in FA profiles and δ13CFA values between i-POM and p-POM and seasonally within p-POM. We demonstrated that these results can be explained as differences and changes in the composition of diatoms, flagellates, and bacteria using FA biomarkers. Concentrations of FA within the bottom 1 cm of sea ice were much greater than those found in the water column, making the sea ice a concentrated source of PUFA for marine grazers. In i-POM, the FAs became more enriched in 13C with increasing day length, and we suggest that the decreased isotopic discrimination during photosynthesis in the carbon-limited and semi-closed sympagic habitat influences this pattern. Finally, we estimated that between 12 and 73 % of sea water DIC was used for FA synthesis in i-POM.

The marked differences in diatom versus flagellate composition in sympagic and pelagic community POM in 2010 and the higher nutritional content within the bottom 1 cm of sea ice compared to within the water column may have implications for marine grazers and possibly higher trophic levels. Of the regions in the Arctic and sub-Arctic, the Bering Sea exhibits the largest seasonal sea ice advance and retreat (Walsh and Johnson 1979; Niebauer 1983; Minobe 2002; Bluhm and Gradinger 2008). By 2050, the spring sea ice extent in the eastern Bering Sea is predicted to be 58 % of the 1980–1999 mean (Wang et al. 2012). As a result of this overall loss of sea ice and early ice retreat due to climate change, a reduction of sea ice primary productivity is predicted to occur (e.g., Bluhm and Gradinger 2008). Additionally, climate-related changes in the timing of seasonal sea ice cover affect spring primary production patterns (Stabeno et al. 2001, 2010; Hunt and Stabeno 2002). These changes in timing of sea ice retreat, predicted loss of sea ice and sea primary productivity, along with increased light intensities may lead to a reduction in high-quality PUFA from i-POM available for sympagic marine organisms and could have profound effects on ice-associated fauna, and possibly on upper trophic level consumers that rely on them as a food resource. The characteristics of FA in i-POM and differences from p-POM elucidated in our study will aid efforts to track the quantitative importance of sea ice algae FA to higher trophic levels in the Bering Sea.

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Acknowledgments

This project was funded by the National Science Foundation (ARC-0902177 and 0732767). Financial support for S. Wang was also provided by the North Pacific Research Board Graduate Research Award, the University of Alaska Center (UAF) for Global Change Student Research Grant with funds from the Cooperative Institute for Alaska Research, Robert Byrd Award, Dieter Family Marine Science Research Scholarship, and the Ken Turner Memorial Fellowship. We thank J. Weems and S. Brennan (UAF) for assisting with sample collections and A. Timmins (Dalhousie) for laboratory assistance. We also thank T. Howe, N. Haubenstock, C. Graham from the ASIF-UAF for laboratory assistance with stable isotope analyses. We are grateful for the excellent assistance of the crew and captains of the USCGC Polarsea and the UNOLS vessel Thomas G. Thompson, and chief scientists L. Cooper (University of Maryland Center for Environmental Science), C. Ashjian (Woods Hole Oceanographic Institution), and D. Shull (Western Washington University). We also thank E. Lessard (University of Washington) for assistance with phytoplankton identifications. Sea ice concentration data were sourced from the model explorer provided by the Alaska Ocean Observing System. Finally, we thank D. O’Brien, L. Horstmann-Dehn, A. Springer, and L. Oxtoby for helpful discussions and comments, and three anonymous reviewers for their detailed and constructive comments which improved our manuscript.

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Correspondence to Shiway W. Wang.

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Communicated by Ulrich Sommer.

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Wang, S.W., Budge, S.M., Gradinger, R.R. et al. Fatty acid and stable isotope characteristics of sea ice and pelagic particulate organic matter in the Bering Sea: tools for estimating sea ice algal contribution to Arctic food web production. Oecologia 174, 699–712 (2014). https://doi.org/10.1007/s00442-013-2832-3

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Keywords

  • Compound-specific carbon stable isotope
  • Biomarkers
  • Trophic studies
  • Marine phytoplankton
  • Arctic