Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Corpuscles of Stannius and stanniocalcin-like immunoreactivity in the white sucker (Catostomus commersoni): evidence for a new cell-type

  • 38 Accesses

  • 6 Citations

Abstract 

The distribution of stanniocalcin immunoreactivity was examined in the corpuscles of Stannius of the white sucker (Catostomus commersoni) by using a chum salmon stanniocalcin antiserum, Western blotting, and light and electron microscopy. The white sucker possesses at least two stanniocalcin-immunoreactive corpuscles in the most posterior portions of the kidneys. Immunocytochemistry and ultrastructure revealed two cell-types in the corpuscle parenchyma, only one of which was immunoreactive. The nonimmunoreactive cells contained dense-cored vesicles and long processes that extended between the immunoreactive cells and terminated at perivascular spaces. When corpuscle extracts were subjected to electrophoresis and Western blotting, three nonreduced stanniocalcin-like immunoreactive bands (approximately 56, 61, and 64 kDa) were observed. However, in the presence of a reductant, a diffuse band migrating in the range of 28 to 32 kDa was noted. The results of this study on the white sucker demonstrate the presence of a dimeric stanniocalcin-like molecule and present evidence of a previously uncharacterized cell-type in the corpuscles of Stannius.

This is a preview of subscription content, log in to check access.

Author information

Additional information

Received: 31 March 1997 / Accepted: 2 February 1998

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Marra, L., Butler, D., Zhang, D. et al. Corpuscles of Stannius and stanniocalcin-like immunoreactivity in the white sucker (Catostomus commersoni): evidence for a new cell-type. Cell Tissue Res 293, 155–164 (1998). https://doi.org/10.1007/s004410051107

Download citation

  • Key words Corpuscles of Stannius
  • Stanniocalcin
  • Immunohistochemistry
  • Neuroendocrine cell
  • Western blotting
  • Catostomus commersoni (Teleostei)