The present study demonstrates the immunocytochemical localisation of the biogenic amine, histamine (HA), in interneurons within the ventral nerve cord of the cricket, Gryllus bimaculatus. Analysis of whole-mount preparations combined with histology of serial sections reveals a constant number of HA-immunoreactive (HA-ir) neurons in the suboesophageal (n=8), thoracic (n=4) and abdominal ganglia (females/males n=24/20). Except for the suboesophageal and prothoracic ganglion, each thoracic and abdominal neuromere contains one pair of bilateral-symmetric HA-ir somata in a medio-ventral position. Axons from HA-ir cells in the thorax extend anteriorly and share common projection areas in thoracic associative neuropils; they terminate in the brain. HA-ir cells also display efferent descending axons. Extending posteriorly, these axons give rise to varicose HA-ir fibre plexuses on the surface of nerve 1 of the abdominal ganglia. In the suboesophageal ganglion, processes from a bilateral symmetric group of clustered HA-ir cells ascend into the tritocerebrum of the brain and further project into the frontal ganglion and the recurrent nerve. Ultrastructural analysis reveals dense-core vesicles, indicative of non-synaptic secretion, in HA-ir elements within the stomatogastric nervous system. Arborisations of HA-ir neurons are present in all major neuropil regions of the ventral nerve cord and display characteristic varicose structures also detected in other types of amine-containing cells. Central HA-ir varicose projections in dorsal and ventral neuropils are located in close apposition to the ganglionic surface. The wide-spread innervation of all neuromeres by HA-ir interneurons and the identification of possible neurohemal release sites suggest a general role of HA as a neuroactive substance, including neuromodulatory and neurohormonal functions.
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Received: 16 February 1996 / Accepted: 27 May 1996
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Hörner, M., Helle, J. & Schürmann, F. The distribution of histamine-immunoreactive neurons in the ventral nerve cord of the cricket, Gryllus bimaculatus . Cell Tissue Res 286, 393–405 (1996). https://doi.org/10.1007/s004410050709
- Key words: Histamine
- Nervous systems
- Gryllus bimaculatus (Insecta)