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Characterization of the olfactory system of the giant honey bee, Apis dorsata

  • Sandhya Mogily
  • Meenakshi VijayKumar
  • Sunil Kumar Sethy
  • Joby JosephEmail author
Regular Article
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Abstract

Apis dorsata is an open-nesting, undomesticated, giant honey bee found in southern Asia. We characterized a number of aspects of olfactory system of Apis dorsata and compared it with the well-characterized, western honeybee, Apis mellifera, a domesticated, cavity-nesting species. A. dorsata differs from A. mellifera in nesting behavior, foraging activity, and defense mechanisms. Hence, there can be different demands on its olfactory system. We elucidated the glomerular organization of A. dorsata by creating a digital atlas for the antennal lobe and visualized the antennal lobe tracts and localized their innervations. We showed that the neurites of Kenyon cells with cell bodies located in a neighborhood in calyx retain their relative neighborhoods in the pedunculus and the vertical lobe forming a columnar organization in the mushroom body. The vertical lobe and the calyx of the mushroom body were found to be innervated by extrinsic neurons with cell bodies in the lateral protocerebrum. We found that the species was amenable to olfactory conditioning and showed good learning and memory retention at 24 h after training. It was also amenable to massed and spaced conditioning and could distinguish trained odor from an untrained novel odor. We found that all the above mentioned features in A. dorsata are very similar to those in A. mellifera. We thereby establish A. dorsata as a good model system, strikingly similar to A. mellifera despite the differences in their nesting and foraging behavior.

Keywords

Apis dorsata Olfactory system Digital atlas Mushroom body Olfactory conditioning 

Notes

Acknowledgements

We would like to thank Uttam Krishna Sharma for his support in procuring Apis dorsata and Shilpi Singh for her support in carrying out electrophysiology and in editing the manuscript. We thank Ravindra Kumar Pydi (National Institute of Rural Development, Hyderabad) for providing Apis mellifera bees. We also thank Prasad Miriyala (Central Instruments Laboratory, University of Hyderabad) and Nalini Manthapuram (Centre for Nanotechnology, University of Hyderabad) for their support in confocal imaging. We are grateful to the UPE scheme of University Grants Commission, India, and DST Purse for providing funding to the University of Hyderabad.

Funding

The study was funded by UPE-UGC, CSIR, and DST Purse.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Centre for Neural and Cognitive SciencesUniversity of HyderabadHyderabadIndia
  2. 2.Bhabha Atomic Research CentreMumbaiIndia

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