Healing potential of injectable Aloe vera hydrogel loaded by adipose-derived stem cell in skin tissue-engineering in a rat burn wound model
- 143 Downloads
Adipose stem cells (ASCs) are a great promise in wound healing due to their potential in differentiating into various cell lineages and secreting growth factors. The purpose of this study is to evaluate the in vivo effects of Aloe vera hydrogel loaded by allogeneic ASCs on a rat burn wound model. The ASCs were isolated, cultured and mixed with 50% Aloe vera hydrogel and injected intradermally around the wound. Demineralized bone matrix (DBM) was used as dressing in the experiment. The burn wound-healing properties of different experimental groups were investigated by histopathological, molecular, scanning electron microscopic and biochemical analysis at the 7th, 14th and 28th days post-wounding. The Aloe vera and DBM-Aloe vera groups showed almost similar healing properties, while treatment by DBM-Aloe vera/ASCs significantly enhanced wound healing. The levels of transforming growth factor-β1 (TGF-β1) and interleukin-1β markedly decreased at the 7th day post-injury, in the DBM-Aloe vera/ASC-treated group, suggesting that this treatment regime subsided the inflammatory responses. Angiogenesis, re-epithelialization and the level of TGF-β1 in the wounds treated with DBM-Aloe vera/ASCs were also remarkably higher than those of other groups, at the 14th day post-injury. Besides, scar formation significantly decreased in the DBM-Aloe vera/ASC-treated wounds when compared with other groups. Our biochemical results were in agreement with the molecular and histopathological findings and strongly demonstrated that a DBM-Aloe vera/ASC composite can stimulate burn wound healing. These results suggest that the DBM-Aloe vera/ASC composite can be considered as a promising therapeutic strategy in the treatment of burn wounds.
KeywordsAdipose stem cell Aloe vera Demineralized bone matrix Burn wound model Histopathology Real-time PCR
The authors would like to thank the authorities of the Veterinary School, Shiraz University, for their kind cooperation.
INSF provided financial support (grant number 96006039).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Castiglione F, Hedlund P, Van der Aa F, Bivalacqua TJ, Rigatti P, Van Poppel H, Montorsi F, De Ridder D, Albersen M (2013) Intratunical injection of human adipose tissue-derived stem cells prevents fibrosis and is associated with improved erectile function in a rat model of Peyronie’s disease. Eur Urol 63:551–560CrossRefGoogle Scholar
- Ebrahimian TG, Pouzoulet F, Squiban C, Buard V, André M, Cousin B, Gourmelon P, Benderitter M, Casteilla L, Tamarat R (2009) Cell therapy based on adipose tissue-derived stromal cells promotes physiological and pathological wound healing. Arterioscler Thromb Vasc Biol 29:503–510CrossRefGoogle Scholar
- Gomez JMQ, Mora RMS, Diaz AC, De Castro MDL (2012) Use of olive leaf extracts in a pharmaceutical composition for inducing angiogenesis and vasculogenesis: Google Patents US 20120141435 A1. In: Patents. Available from: https://www.google.com/patents/US20120141435. Accessed 18 Nov 2015
- Joseph B, Raj SJ (2010) Pharmacognostic and phytochemical properties of Aloe vera Linn: an overview. Int J Pharm Sci Rev Res 4:106–110Google Scholar
- Kato Y, Iwata T, Washio K, Yoshida T, Kuroda H, Morikawa S, Hamada M, Ikura K, Kaibuchi N, Yamato M (2017) Creation and transplantation of an adipose-derived stem cell (ASC) sheet in a diabetic wound-healing model. J Vis Exp. https://doi.org/10.3791/54539
- Oryan A, Naeini AT, Nikahval B, Gorjian E (2010) Effect of aqueous extract of Aloe vera on experimental cutaneous wound healing in rat. Veterinarski Arhiv 80:509–522Google Scholar
- Oryan A, Moshiri A, Meimandiparizi A-H (2011) Effects of sodium-hyaluronate and glucosamine-chondroitin sulfate on remodeling stage of tenotomized superficial digital flexor tendon in rabbits: a clinical, histopathological, ultrastructural, and biomechanical study. Connect Tissue Res 52:329–339CrossRefGoogle Scholar
- Sun G, Zhang X, Shen Y-I, Sebastian R, Dickinson LE, Fox-Talbot K, Reinblatt M, Steenbergen C, Harmon JW, Gerecht S (2011) Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing. Proc Natl Acad Sci U S A 108:20976–20981CrossRefPubMedCentralGoogle Scholar
- Wang J, Hao H, Huang H, Chen D, Han Y, Han W (2016) The effect of adipose-derived stem cells on full-thickness skin grafts. Biomed Res Int 2016:146472Google Scholar