Oxidative stress modulates the expression of apoptosis-associated microRNAs in bovine granulosa cells in vitro
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Despite its essential role in ovulation, oxidative stress (OS) has been found to be cytotoxic to cells, while microRNAs (miRNAs) are known as a major regulator of genes involved in cellular defense against cytotoxicity. However, a functional link between OS and miRNA expression changes in granulosa cells (GCs) remains to be investigated. Here, we investigate the OS modulation of apoptosis-associated miRNAs and their biological relevance in bovine GCs. Following the evaluation of cell viability, accumulation of reactive oxygen species (ROS), cytotoxicity and mitochondrial activity, we used a ready-to-use miRNA PCR array to identify differentially regulated miRNAs. The results showed that exposure to 150 μM H2O2 for 4 h creates remarkable signs of OS in GCs characterized by more than 50% loss of cell viability, higher nuclear factor erythroid 2–related factor 2 (NRF2) nuclear translocation, significantly (p < 0.05) higher abundance of antioxidant genes, significantly (p < 0.001) higher accumulation of ROS, lower mitochondrial activity and a higher (p < 0.001) number of apoptotic nuclei compared to that of the control group. miRNA expression analysis revealed that a total of 69 miRNAs were differentially regulated in which 47 and 22 miRNAs were up- and downregulated, respectively, in stressed GCs. By applying the 2-fold and p < 0.05 criteria, we found 16 miRNAs were upregulated and 10 miRNAs were downregulated. Target prediction revealed that up- and downregulated miRNAs potentially targeted a total of 6210 and 3575 genes, respectively. Pathway analysis showed that upregulated miRNAs are targeting the genes involved mostly in cell survival, intracellular communication and homeostasis, cellular migration and growth control and disease pathways. Our results showed that OS modulates the expression of apoptosis-associated miRNAs that might have effects on cellular or molecular damages.
KeywordsmicroRNA Oxidative stress Granulosa cells Apoptosis Signaling pathways
The authors are indebted to Res. Asst. Mahmut Kaliber; Sebahattin Koknur, DVM; and Ali Ergin, DVM, for their assistance during sample collection.
This research was supported by the Erciyes University Scientific Research Projects Coordination Unit, Project No: FOA-2015-5655.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Statement on the welfare of animals
This article does not contain any studies with live animals performed by any of the authors.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Agarwal A, Gupta S, Sharma RK (2005) Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 3(28). https://doi.org/10.1186/1477-7827-3-28
- Du J-Y, Wang L-F, Wang Q, Yu L-D (2015) miR-26b inhibits proliferation, migration, invasion and apoptosis induction via the downregulation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 driven glycolysis in osteosarcoma cells. Oncol Rep 33:1890–1898. https://doi.org/10.3892/or.2015.3797 CrossRefPubMedGoogle Scholar
- Hung C-M, Garcia-Haro L, Sparks CA, Guertin DA (2012) mTOR-dependent cell survival mechanisms. Cold Spring Harb Perspect Biol. https://doi.org/10.1101/cshperspect.a008771
- Ngamwongsatit P, Banada PP, Panbangred W, Bhunia AK (2008) WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line. J Microbiol Methods 73:211–215. https://doi.org/10.1016/j.mimet.2008.03.002 CrossRefPubMedGoogle Scholar
- Sohel MMH, Hoelker M, Noferesti SS et al (2013) Exosomal and non-exosomal transport of extra-cellular microRNAs in follicular fluid: implications for bovine oocyte developmental competence. PLoS One. https://doi.org/10.1371/journal.pone.0078505
- Sohel MMH, Konca Y, Akyuz B et al (2017) Concentration dependent antioxidative and apoptotic effects of sulforaphane on bovine granulosa cells in vitro. Theriogenology 97:17–26. https://doi.org/10.1016/j.theriogenology.2017.04.015 CrossRefPubMedGoogle Scholar
- Sohel MMH, Amin A, Prastowo S, et al (2018) Sulforaphane protects granulosa cells against oxidative stress via activation of NRF2-ARE pathway. Cell Tissue Res 1–13. https://doi.org/10.1007/s00441-018-2877-z
- Yuan S, Ortogero N, Wu Q et al (2014) Murine follicular development requires oocyte DICER, but not DROSHA1. Biol Reprod. https://doi.org/10.1095/biolreprod.114.119370
- Zhang Y-L, Wang R-C, Cheng K et al (2017b) Roles of Rap1 signaling in tumor cell migration and invasion. Cancer Biol Med 14:90–99. https://doi.org/10.20892/j.issn.2095-3941.2016.0086 CrossRefPubMedPubMedCentralGoogle Scholar