Cell and Tissue Research

, Volume 376, Issue 1, pp 137–141 | Cite as

Morphometric analysis of thoracic aorta in Slc39a13/Zip13-KO mice

  • Takuya Hirose
  • Takamasa Shimazaki
  • Naoki Takahashi
  • Toshiyuki Fukada
  • Takafumi Watanabe
  • Prasarn TangkawattanaEmail author
  • Kazushige Takehana
Short Communication


Ehlers–Danlos syndrome (EDS) is a collection of inheritable diseases involving the musculoskeletal, integumentary and visual systems. Spondylodysplastic EDS-ZIP13 (spEDS-ZIP13: OMIM 612350) was recently defined as a new form of EDS. Although vasculitis has been found in many spEDS-ZIP13 patients, vascular pathology has not been included as a pathognomonic lesion of this type of EDS. We investigate the morphometry of the thoracic aorta in wild-type and Zip13-knockout (Zip13-KO) mice. Our assessment found abnormalities in the number and morphology of elastic and cellular components in the aortic wall, especially the tunica media, of Zip13-KO mice, indicating aortic fragility. Accordingly, our major findings (vascular smooth muscle cells with small nuclei, small percentage of elastic membrane area per tunica media, many large elastic flaps) should be considered vulnerable characteristics indicating fragility of the aorta in patients with spEDS-ZIP13.


Aorta Elasticity Elastic membrane Morphometry Slc39a13/Zip13-KO mouse 



The authors express sincere gratitude to Drs. Hiromi Ueda and Jun Minaguchi of the Laboratory of Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan, for their technical advice.

Funding information

This work was partially supported by The Maple Foundation.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Takuya Hirose
    • 1
  • Takamasa Shimazaki
    • 1
  • Naoki Takahashi
    • 1
  • Toshiyuki Fukada
    • 2
  • Takafumi Watanabe
    • 1
    • 3
  • Prasarn Tangkawattana
    • 1
    • 4
    Email author
  • Kazushige Takehana
    • 1
  1. 1.Laboratory of Anatomy, School of Veterinary MedicineRakuno Gakuen UniversityEbetsuJapan
  2. 2.Molecular and Cellular Physiology, Faculty of Pharmaceutical SciencesTokushima Bunri UniversityTokushimaJapan
  3. 3.Laboratory of Animal Functional Anatomy, Faculty of AgricultureShinshu UniversityNaganoJapan
  4. 4.Faculty of Veterinary MedicineKhon Kaen UniversityKhon KaenThailand

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