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Testosterone improves the osteogenic potential of a composite in vitro and in vivo

  • Kelen J. R. da Costa
  • Alfonso Gala-García
  • Joel J. Passos
  • Vagner R. Santos
  • Ruben D. Sinisterra
  • Célia R. M. Lanza
  • Maria E. Cortés
Regular Article
  • 16 Downloads

Abstract

Testosterone (T) has been suggested as a promising agent in the bone osteointegration when incorporated in a bioceramic/polymer combination for the local application. The objective of this study was to evaluate the activity of a testosterone composite of poly (lactic-co-glycolic acid) (PLGA), polycaprolactone (PCL), and biphasic calcium phosphate (BCP) as a strategy for enhancing its osteogenic effect and to evaluate tissue response to the composite implantation. PLGA/PCL/BCP/T and PLGA/PCL/BCP composites were prepared and characterized using thermal analysis. Composite morphology and surface characteristics were assessed by SEM and EDS. The evaluations of in vitro effects of testosterone composite on osteoblasts viability, alkaline phosphatase activity, collagen production, osteocalcin concentration, quantification of mineralization, and nitric oxide concentration, after 7, 14, and 21 days. Testosterone was successfully incorporated and composites showed a homogeneously distributed porous structure. The PLGA/PCL/BCP/T composite had a stimulatory effect on osteoblastic activity on the parameters evaluated, except to nitric oxide production. After 60 days, the PLGA/PCL/BCP/T composite showed no chronic inflammatory infiltrate, whereas the PLGA/PCL/BCP composite showed mild chronic inflammatory infiltrate. Angiogenesis, cellular adsorption, and fibrous deposit were observed on the surfaces of implanted composites. The composites in combination with testosterone can be exploited to investigate the use of this scaffold for bone integration.

Keywords

Testosterone Bone formation Calcium phosphate Ceramic composite Mineralization 

Notes

Acknowledgements

The authors are grateful to the Chemistry Department of UFMG for the use of their Thermal Spectroscopy and Center of Microscopy (UFMG) for providing the equipment.

Funding

This study had financial support by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Instituto Nacional de Ciência e Tecnologia Nanobiofar (INCT-Nanobiofar) CNPq Brazilian agencies.

Compliance with ethical standards

Conflict of interest

The authors declare that they do not have any conflict of interests.

Supplementary material

441_2018_2970_MOESM1_ESM.jpg (49 kb)
Supplemental Figure EDS analysis of the surfaces of a) PLGA/PCL/BCP/T and b) PLGA/PCL/BCP/T after soaking in SBF for 21 days, and c) PLGA/PCL/BCP after soaking in SBF for 21 days. (JPG 48 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Kelen J. R. da Costa
    • 1
  • Alfonso Gala-García
    • 1
  • Joel J. Passos
    • 2
  • Vagner R. Santos
    • 3
  • Ruben D. Sinisterra
    • 2
  • Célia R. M. Lanza
    • 3
  • Maria E. Cortés
    • 1
  1. 1.Restorative Dentistry Department, Dentistry FacultyUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  2. 2.Department of ChemistryUniversidade Federal de Minas GeraisBelo HorizonteBrazil
  3. 3.Departmentof Dental Clinic, Pathology and Surgery, Dentistry FacultyUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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