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Cell and Tissue Research

, Volume 375, Issue 2, pp 531–541 | Cite as

Expression of PTGS2, PGFS and PTGFR during downregulation and restart of spermatogenesis following GnRH agonist treatment in the dog

  • H. Körber
  • S. Goericke-PeschEmail author
Regular Article
  • 82 Downloads

Abstract

Prostaglandins (PGs) and prostaglandin endoperoxide synthase (PTGS) are considered to be relevant for spermatogenesis and steroidogenesis. PTGS2, prostaglandin F synthase (PGFS) and PGF receptor (PTGFR) are investigated in the adult male dog using the model of the GnRH-agonist implant downregulated canine testis and its subsequent restart of spermatogenesis following abolition of treatment (3, 6, 9, 12 weeks after implant removal). On the mRNA level (ratio), PTGS2, PGFS and PTGFR expression did not differ between downregulation, different stages of recovery of spermatogenesis and untreated adult controls (CG). On the protein level, Sertoli and Leydig cells in all samples and some peritubular cells stained immunopositive for PTGS2. In the tubular compartment, the percentage of the PTGS2-immunopositive area (PIA) and the mean PTGS2-staining intensity (gray scale, GS) did not differ between groups but in the interstitial compartment, the PIA (p = 0.0494) and the GS (p < 0.0001) were significantly upregulated during early recrudescence (week 3/6). Comparing downregulation by two GnRH-agonist implants with juvenile controls (JG) and CG, the mRNA expression (ratio) did not differ. In the tubular compartment, the GS (p = 0.0321) was significantly higher at downregulation compared to CG and in the interstitial compartment, the PIA (p = 0.0073) and the GS (p = 0.0097) were significantly higher in JG compared to downregulation/CG. PTGS2, PGFS and PTGFR mRNA and PTGS2 protein are regularly expressed in the adult, juvenile and downregulated canine testis and downregulation and subsequent recrudescence affect PTGS2 protein expression mainly in Leydig cells. PTGS2 expression in the downregulated testis resembles the one in seasonal Syrian hamster but not juvenile canine testis.

Keywords

PTGS2 PGFS PTGFR Prostaglandin Slow release GnRH agonist implant 

Notes

Acknowledgements

The financial support of the project by the Dr. Jutta & Dr. Georg Bruns Stiftung is gratefully acknowledged. The authors also would like to thank Dr. Gabi Itter (Sanofi Aventis, Frankfurt) for providing the beagle dogs and Intervet Pharma R&D, Angers Technopole, France, for provision of the Gonazon® implant. Finally, we are thankful to those people who took the dogs in their custody for their great collaboration.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Statement of the welfare of animals

All applicable national and institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted.

Animal experimentation had been approved by the respective authority (permit no. AZ V54-19c20/15c GI18/14, Regierungspräsidium Gießen).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Veterinary Clinical Sciences, Section for Veterinary Reproduction and Obstetrics, Faculty of Health and Medical SciencesUniversity of CopenhagenTåstrupDenmark
  2. 2.Reproductive Unit of the Clinics—Clinic for Small AnimalUniversity of Veterinary Medicine HannoverHannoverGermany

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