Morphology of visual projection neurons supplying premotor area in the brain of the silkmoth Bombyx mori
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Sex pheromones orient male moths toward conspecific female moths; the presence of visual information modulates this behavior. In the current study, we explore candidate neuronal pathways for the interaction between vision and the locomotor signal for pheromone orientation. We describe the connectivity between visual neuropils and brain premotor centers, the posterior slope (PS) and the lateral accessory lobe (LAL), in the silkmoth Bombyx mori. Using a single-cell labeling technique, we analyze visual projection neurons supplying these areas. Neurons from both the medulla and lobula complex projected to the PS but only the neurons originating in the lobula complex had additional processes to the LAL. Further, we identified populations of putative feedback neurons from the premotor centers to the optic lobe. Neurons originating in the PS were likely to project to the medulla, whereas those originating in the LAL were likely to project to the lobula complex. The anatomical study contributes to further understanding of integration of visual information on the locomotor control in the insect brain.
KeywordsIntracellular recording Lateral accessory lobe Posterior slope Lobula Pheromone
Lateral accessory lobe
Lateral accessory lobe commissure
Lateral antennal lobe tract
Peduncles of the mushroom body
Posterior lateral protocerebrum
Superior medial protocerebrum
Ventral lateral protocerebrum
Delta area of the inferior lateral protocerebrum
We thank Chika Iwatsuki, Satoshi Wada and Evan S. Hill for their technical assistance. We are grateful to the FlyCircuit database from the NCHC (National Center for High-performance Computing) and NTHU (National Tsing Hua University).
This work was financially supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Grant numbers: 17H05011 and 16H06732 to SN, 15H04399 to R.K.) and the Narishige Zoological Science Award to S.N.
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