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Morphology of visual projection neurons supplying premotor area in the brain of the silkmoth Bombyx mori

  • Shigehiro Namiki
  • Ryohei Kanzaki
Regular Article
  • 224 Downloads

Abstract

Sex pheromones orient male moths toward conspecific female moths; the presence of visual information modulates this behavior. In the current study, we explore candidate neuronal pathways for the interaction between vision and the locomotor signal for pheromone orientation. We describe the connectivity between visual neuropils and brain premotor centers, the posterior slope (PS) and the lateral accessory lobe (LAL), in the silkmoth Bombyx mori. Using a single-cell labeling technique, we analyze visual projection neurons supplying these areas. Neurons from both the medulla and lobula complex projected to the PS but only the neurons originating in the lobula complex had additional processes to the LAL. Further, we identified populations of putative feedback neurons from the premotor centers to the optic lobe. Neurons originating in the PS were likely to project to the medulla, whereas those originating in the LAL were likely to project to the lobula complex. The anatomical study contributes to further understanding of integration of visual information on the locomotor control in the insect brain.

Keywords

Intracellular recording Lateral accessory lobe Posterior slope Lobula Pheromone 

Abbreviations

CB

Central body

CX

Central complex

DNs

Descending neurons

ES

Esophagus

GNG

Gnathal ganglion

ILo

Inner lobula

LAL

Lateral accessory lobe

LALC

Lateral accessory lobe commissure

lALT

Lateral antennal lobe tract

LH

Lateral horn

LP

Lobula plate

LY

Lucifer yellow

Me

Medulla

MGC

Macroglomerular complex

OLo

Outer lobula

Pe

Peduncles of the mushroom body

PLP

Posterior lateral protocerebrum

PS

Posterior slope

SMP

Superior medial protocerebrum

VLP

Ventral lateral protocerebrum

VPC

Ventral protocerebrum

ΔILPC

Delta area of the inferior lateral protocerebrum

Notes

Acknowledgements

We thank Chika Iwatsuki, Satoshi Wada and Evan S. Hill for their technical assistance. We are grateful to the FlyCircuit database from the NCHC (National Center for High-performance Computing) and NTHU (National Tsing Hua University).

Funding information

This work was financially supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Grant numbers: 17H05011 and 16H06732 to SN, 15H04399 to R.K.) and the Narishige Zoological Science Award to S.N.

Supplementary material

441_2018_2892_MOESM1_ESM.dtd (42 kb)
ESM 1 (DTD 42 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Research Center for Advanced Science and TechnologyThe University of TokyoTokyoJapan

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