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Cell and Tissue Research

, Volume 376, Issue 2, pp 179–187 | Cite as

Dental pulp stem cell transplantation ameliorates motor function and prevents cerebellar atrophy in rat model of cerebellar ataxia

  • Abbas Aliaghaei
  • Mahdi Eskandarian Boroujeni
  • Houssein Ahmadi
  • Amir-Hossein Bayat
  • Mostafa Rezaei Tavirani
  • Mohammad Amin Abdollahifar
  • Mohammad H. Pooyafar
  • Vahid MansouriEmail author
Regular Article

Abstract

Cerebellar ataxias (CA) include a range of neurodegenerative disorders hallmarked by deterioration of the cerebellum. Cell replacement therapy (CRT) offers a potential remedy for the diseases associated with the central nervous system (CNS). This study was designed to assess the neurorestorative/protective effects of dental pulp stem cell (DPSC) implantation on a rat model of CA induced by 3-acetylpyridine (3-AP) as a neurotoxin. To begin, human DPSCs were extracted, cultured and phenotypically characterized. Then, experimental ataxia was induced in 20 male adult rats by a single injection of 3-AP and bilateral DPSC transplantation was performed 3 days after 3-AP administration, followed by stereological analysis of cerebellar layers along with assessment of motor skills and inflammatory response. The findings showed that transplantation of DPSCs in a 3-AP model of ataxia ameliorated motor coordination and muscle activity, increased cerebellar volumes of molecular and granular layers plus white matter, reduced the levels of inflammatory cytokines and thwarted the degeneration of Purkinje cells against 3-AP toxicity. Taken together, human DPSCs could be considered as a suitable candidate for CRT-based therapies with a specific focus on CA.

Keywords

Cerebral ataxia Dental pulp stem cells Motor activity Cerebellar volume 3-Acetylpyridine 

Notes

Acknowledgments

This research project was performed at the Cell Biology & Anatomical Sciences Department, Shahid Beheshti University of Medical Sciences.

Funding information

This research project was supported by the Vice Chancellor of Research of the Shahid Beheshti University of Medical Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Ethical approval

All procedures performed in the present study involving humans and animals were in accordance with the ethical standards of the Ethical Committee of Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Supplementary material

441_2018_2980_MOESM1_ESM.docx (101 kb)
ESM 1 (DOCX 100 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Abbas Aliaghaei
    • 1
  • Mahdi Eskandarian Boroujeni
    • 2
  • Houssein Ahmadi
    • 1
  • Amir-Hossein Bayat
    • 3
  • Mostafa Rezaei Tavirani
    • 4
  • Mohammad Amin Abdollahifar
    • 1
  • Mohammad H. Pooyafar
    • 5
  • Vahid Mansouri
    • 6
    Email author
  1. 1.Cell Biology & Anatomical Sciences Department, School of MedicineShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Department of Stem Cells & Regenerative Medicine, Faculty of Medical BiotechnologyNational Institute of Genetic Engineering & BiotechnologyTehranIran
  3. 3.Department of Neurobiology and NeuropsychologySaveh University of Medical SciencesSavehIran
  4. 4.Proteomics Research CenterShahid Beheshti University of Medical SciencesTehranIran
  5. 5.School of MedicineShahid Beheshti University of Medical SciencesTehranIran
  6. 6.Faculty of Paramedical Science, Proteomics Research CenterShahid Beheshti University of Medical SciencesTehranIran

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